Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

Products

AnaTag™ Protein Labeling Kits

Europium Labeling Kits for TR-FRET

Europium
AnaTag Kit
Stokes-Shift-Eu

Stokes Shift Absorption and Emission Peaks for Europium
Stokes shift is the difference between the absorption peak and the emission peak for fluorophores. A larger Stokes shift is always preferred which allows the use of broad excitation and emission filters that do not overlap, which in turn increases brightness and sensitivity. Fluorescent probes of smaller Stokes shift require filters that are very close together and do not include the entire area of the curves, thus reducing efficiency and brightness.

Europium Labeling

Schematic representation of TR-FRET energy transfer from Eu-labeled donor to HiLyte™ Fluor 647-labeled acceptor.

AnaTag-Labeling

AnaTag™ Labeling Kits for TR-FRET
Conveniently conjugate your own TR-FRET Protein reagents with the amine reactive Europium chelate and HiLyte™ Fluor 647 labeling kits.

Europium-Chelate-HiLyte-Fluor647

Features

  • Fast labeling
  • Stable dye-protein conjugates
  • All essential components for conjugation and purification included
  • Ideal for HTS

Description

  • Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) involves an energy transfer between two fluorophores in close proximity. It is a combination of Time Resolved Fluorescence (TRF) and Forster‘s Resonance Energy Transfer (FRET). This method shows reduced background noise and higher sensitivity and reliability than FRET. In TR-FRET the donor molecule is a Lanthanide metal instead of a fluorescent dye. Lanthanide metals, such as Terbium (Tb) and Europium (Eu), serve as energy donors. Their fluorescence is long-lived and characterized by a large Stokes shift providing a high signal-to-noise ratio.

Application

  • FRET/TR-FRET substrates for protease activity detection
  • Protease-based drug target screening and discovery
  • Protein-peptide interaction
  • Protein-protein interaction

Kit Overview

Label Kit Description Ex/Em Order#
Europium Chelate AnaTag™ Europium Protein Labeling Kit 325/620 nm 72246AS
HiLyte™ Fluor 647 AnaTag™ HiLyte™ Fluor 647 Protein Labeling Kit 649/674 nm 72049AS
HiLyte™ Fluor 647 AnaTag™ HiLyte™ Fluor 647 Microscale Protein Labeling Kit 649/674 nm 72050AS
Europium Chelate & HiLyte™ Fluor 647 AnaTag™ Protein Labeling Kit for TR-FRET Eu: 325/620 nm
HL 647: 649/674 nm
72247AS

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
72246AS AnaTag™ Europium Protein Labeling Kit *Ultra Convenient* 1 kit 909,00 PDF
72247AS AnaTag™ Protein Labeling Kit for TR-FRET *Ultra Convenient* 1 kit 1023,00 PDF
72049AS AnaTag™ HiLyte™ Fluor 647 Protein Labeling Kit *Ultra Convenient* [3 labeling reactions (3 x 5 mg protein)] 1 kit 387,00 PDF
72050AS AnaTag™ HiLyte™ Fluor 647 Microscale Protein Labeling Kit *Ultra Convenient* 1 kit 243,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in Germany and selected European countries.

Download

  • AnaSpec: TR-FRET Flyer (PDF)