Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

Products

Lipid Strips and Arrays from Echelon

TMB Precipitating

Description

  • TMB Precipitating (K-TMBP) is a chromogen substrate that reacts with horseradish peroxide (HRP) producing a permanent purple colored spot or band. This detection reagent is best used with an HRP conjugated secondary or tertiary antibody on a dot blot or western blot. We recommend K-TMBP for use with strips, arrays, and mass strip kits.

PIP Strips

PIP Strips

Description

  • PIP Strips are 2 x 6 cm hydrophobic membranes that have been spotted with 100 pmol of all eight phosphoinositides and seven other biological important lipids. They are used to determine how your protein of interest interacts with one or more of these lipids in a simple protein-lipid overlay assay. Bound protein is then detected by an antibody against the protein or affinity tag.

PIP Strips contain the following lipids at 100 pmol per spot:

  • Lysophosphatidic acid (LPA)
  • Lysophosphocholine (LPC)
  • Phosphatidylinositol (PtdIns)
  • Phosphatidylinositol (3) phosphate (PtdIns(3)P)
  • Phosphatidylinositol (4) phosphate (PtdIns(4)P)
  • Phosphatidylinositol (5) phosphate (PtdIns(5)P)
  • Phosphatidylethanolamine (PE)
  • Phosphatidylcholine (PC)
  • Sphingosine 1-Phosphate (S1P)
  • Phosphatidylinositol (3,4) bisphosphate (PtdIns(3,4)P2 )
  • Phosphatidylinositol (3,5) bisphosphate (PtdIns(3,5)P2)
  • Phosphatidylinositol (4,5) bisphosphate (PtdIns(4,5)P2)
  • Phosphatidylinositol (3,4,5) trisphosphate (PtdIns(3,4,5)P3)
  • Phosphatidic acid (PA)
  • Phosphatidylserine (PS)
  • Each 10-pack of PIP Strips includes a free vial of the PI(4,5)P2 specific binding protein for use as a positivie control.

Membrane Lipid Strips

Membrane Lipid Strips

Description

  • Membrane Lipid Strips are 2 x 6 cm hydrophobic membranes that have been spotted with 100 pmol of fifteen different biologically important lipids found in cell membranes. They are used to determine how your protein of interest interacts with one or more of these lipids in a simple protein-lipid overlay assay. Bound protein is then detected by an antibody against the protein or affinity tag.

Membrane Lipid Strips contain the following lipids at 100 pmol per spot:

  • Phosphatidylinositol (PtdIns)
  • Phosphatidylinositol (4)-phosphate (PtdIns(4)P)
  • Phosphatidylinositol (4,5)-bisphosphate, (PtdIns(4,5)P2)
  • Phosphatidylinositol (3,4,5)-trisphosphate (PtdIns(3,4,5)P3)
  • Phosphatidylserine (PS)
  • Phosphatidylethanolamine (PE)
  • Phosphatidic acid (PA)
  • Diacylglycerol (DAG)
  • Cholesterol
  • Phosphatidylcholine (PC)
  • Sphingomyelin
  • Phosphatidylglycerol (PG)
  • 3-sulfogalactosylceramide (Sulfatide)
  • Cardiolipin
  • Each 10-pack of Membrane Lipid Strips includes a free vial of the PI(4,5)P2 specific binding protein for use as a positivie control.

Membrane Lipid Arrays

Membrane Lipid Arrays

Membrane Lipid Arrays (order# P-6003)

Description

  • Membrane Lipid Arrays are 4 x 5 cm hydrophobic membranes that have been spotted with a concentration gradient of eight different biologically important lipids found in cell membranes. They are used to determine the relative degree of binding of your protein of interest towards one or more of these lipids in a simple protein lipid overlay assay. Bound protein is then detected by an antibody against the protein or affinity tag.

Each Membrane Lipid Array has 100, 50, 25, 12.5, 6.25, 3.13, and 1.56 pmol of the following lipids:

  • Diacylglycerol (DAG)
  • Phosphatidic acid (PA)
  • Phosphatidylserine (PS)
  • Phosphatidylinositol (PtdIns)
  • Phosphatidylethanolamine (PE)
  • Phosphatidylcholine (PC)
  • Phosphatidylglycerol (PG)
  • Sphingomyelin

PIP Array

PIP Array

PIP Array (order# P-6100)

Description

  • PIP Arrays are 4 x 5 cm hydrophobic membranes that have been spotted with a concentration gradient of all eight phosphoinositides. They are used to determine the relative degree of binding of your protein of interest towards one or more of these lipids in a simple protein lipid overlay assay. Bound protein is then detected by an antibody against the protein or affinity tag.

Each PIP Array has 100, 50, 25, 12.5, 6.25, 3.13, and 1.56 pmol of the following phosphoinositides:

  • Phosphatidylinositol (PtdIns)
  • Phosphatidylinositol (3) phosphate (PtdIns(3)P)
  • Phosphatidylinositol (4) phosphate (PtdIns(4)P)
  • Phosphatidylinositol (5) phosphate (PtdIns(5)P)
  • Phosphatidylinositol (3,4) bisphosphate (PtdIns(3,4)P2 )
  • Phosphatidylinositol (3,5) bisphosphate (PtdIns(3,5)P2)
  • Phosphatidylinositol (4,5) bisphosphate (PtdIns(4,5)P2)
  • Phosphatidylinositol (3,4,5) trisphosphate (PtdIns(3,4,5)P3)
  • Each 5-pack of PIP Arrays includes a free vial of the PI(4,5)P2 specific binding protein for use as a positivie control.

PIP MicrostripsTM

PIP Microstrips

Description

  • Identical to PIP Strips in form and function, PIP Microstrips were designed to provide the same convenient testing format at a smaller scale. PIP Microstrips are 1x2 cm (PIP Strips are 2 x 6 cm) and can be probed with less than 0.5 ug of protein. Each spot contains 20 pmol of immobilized lipid.

PIP Strips contain the following lipids at 100 pmol per spot:

  • Lysophosphatidic acid (LPA)
  • Lysophosphocholine (LPC)
  • Phosphatidylinositol (PtdIns)
  • Phosphatidylinositol (3) phosphate (PtdIns(3)P)
  • Phosphatidylinositol (4) phosphate (PtdIns(4)P)
  • Phosphatidylinositol (5) phosphate (PtdIns(5)P)
  • Phosphatidylethanolamine (PE)
  • Phosphatidylcholine (PC)
  • Sphingosine 1-Phosphate (S1P)
  • Phosphatidylinositol (3,4) bisphosphate (PtdIns(3,4)P2 )
  • Phosphatidylinositol (3,5) bisphosphate (PtdIns(3,5)P2)
  • Phosphatidylinositol (4,5) bisphosphate (PtdIns(4,5)P2)
  • Phosphatidylinositol (3,4,5) trisphosphate (PtdIns(3,4,5)P3)
  • Phosphatidic acid (PA)
  • Phosphatidylserine (PS)
  • Each 10-pack of PIP Microstrips includes a free vial of the PI(4,5)P2 specific binding protein for use as a positivie control.

PIP Pack

PIP Pack

Description

  • The PIP PACK is a bundle of lipid-protein interaction tests, controls, and a wash case. The pack contains 5 PIP Strips and 2 Membrane Lipid Strips to identify binding, 2 PIP Arrays to quantify binding affinity, 2 vials of PI(4,5)P2 specific binding protein (G-4501) for use as positive controls, and a wash case.

Sphingo Strips

Sphingo Strips

Sphingo Strips (order# S-6000)

Description

  • Sphingo Strips are 2 x 6 cm hydrophobic membranes that have been spotted with 100 pmol of fifteen different sphingolipids. They are used to determine how your protein of interest interacts with one or more of these lipids in a simple protein lipid overlay assay. Bound protein is then detected by an antibody against the protein or affinity tag.

SphingoStrips contain the following lipids at 100 pmol per spot:

  • Sphingosine
  • Sphingosine 1-phosphate (S1P)
  • Phytosphingosine
  • Ceramide
  • Sphingomyelin
  • Sphingosylphosphorylcholine (SPC)
  • Lysophosphatidic Acid (LPA)
  • Myriosine
  • Monosialoganglioside-GM1
  • Disialoganglioside-GD3
  • Sulfatide
  • Psychosine
  • Cholesterol
  • Lysophosphatidylcholine (LPC)
  • Phosphatidylcholine

Sphingo Arrays

Sphingo Arrays

Sphingosine Arrays (order# S-6001)

Description

  • Sphingo Arrays are 4 x 5 cm hydrophobic membranes that have been spotted with a concentration gradient of eight different sphingolipids. They are used to determine the relative degree of binding of your protein of interest towards one or more of these lipids in a simple protein lipid overlay assay. Bound protein is then detected by an antibody against the protein or affinity tag.

Each Sphingo Array has 100, 50, 25, 12.5, 6.25, 3.13, and 1.56 pmol of the following sphingolipids:

  • Sphingosine
  • Sphingosine 1-phosphate (S1P)
  • Sphingosylphosphorylcholine (SPC)
  • Phytosphingosine
  • Ceramide
  • Sphingomyelin
  • Sulfatide
  • Monosialoganglioside-GM1

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
K-TMBP-EC TMB Precipitating 22 ml 52,00 PDF
P-6001-2-EC PIP Strips 2 pk 153,00 PDF
P-6001-10-EC PIP Strips 10 pk 486,00 PDF
P-6002-2-EC Membrane Lipid Strips 2 pk 153,00 PDF
P-6002-10-EC Membrane Lipid Strips 10 pk 486,00 PDF
P-6003-2-EC Membrane Lipid Arrays 2 pk 308,00 PDF
P-6003-5-EC Membrane Lipid Arrays 5 pk 533,00 PDF
P-6100-2-EC PIP Array 2 pk 284,00 PDF
P-6100-5-EC PIP Array 5 pk 498,00 PDF
P-P100-EC PIP PACK 1 ea 500,00 PDF
S-6000-2-EC SphingoStrips 2 strips 153,00 PDF
S-6000-10-EC SphingoStrips 10 strips 415,00 PDF
S-6001-2-EC Sphingo Arrays 2 pk 296,00 PDF
S-6001-5-EC Sphingo Arrays 5 pk 522,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in Europe except for France and Italy.

Download

  • Strips and Arrays Protocol PDF

Literature

  • Huang, S., L. Gao, et al. (2006). "Heterodimeric Capping Protein from Arabidopsis Is Regulated by Phosphatidic Acid." Molecular Biology of the Cell 17(4): 1946-1958.
  • Jin, W., W.-P. Ge, et al. (2006). "Lipid Binding Regulates Synaptic Targeting of PICK1, AMPA Receptor Trafficking, and Synaptic Plasticity." The Journal of Neuroscience 26(9): 2380-2390.
  • Xi, Q., G. J. T. Pauer, et al. (2005). "Tubby-like Protein 1 (TULP1) Interacts with F-actin in Photoreceptor Cells." Investigative Ophthalmology & Visual Science 46(12): 4754-4761.
  • Sharma, V. P., V. Desmarais, et al. (2008). "Immunostaining evidence for PI(4,5)P2 localization at the leading edge of chemoattractant-stimulated HL-60 cells." J Leukoc Biol 84(2): 440-7.
  • Sankarshanan, M., Z. Ma, et al. (2007). "Identification of a novel lipid raft-targeting motif in SRC homology 2-containing phosphatase 1." J Immunol 179(1): 483-90.
  • Lu, B. and C. Benning (2009). "A 25-amino acid sequence of the Arabidopsis TGD2 protein is sufficient for specific binding of phosphatidic acid." J Biol Chem 284(26): 17420-7.
  • Landeta, O., A. Landajuela, et al. (2011). "Reconstitution of proapoptotic BAK function in liposomes reveals a dual role for mitochondrial lipids in the BAK-driven membrane permeabilization process." J Biol Chem 286(10): 8213-30.
  • Dibble, C. F., J. A. Horst, et al. (2010). "Defining the functional domain of programmed cell death 10 through its interactions with phosphatidylinositol-3,4,5-trisphosphate." PLoS One 5(7): e11740.
  • Gozani, O., P. Karuman, et al. (2003). "The PHD finger of the chromatin-associated protein ING2 functions as a nuclear phosphoinositide receptor." Cell 114(1): 99-111.
  • Lindsay, A. J. and M. W. McCaffrey (2004). "The C2 domains of the class I Rab11 family of interacting proteins target recycling vesicles to the plasma membrane." J Cell Sci 117(19): 4365-4375.
  • Andersson, E., F. Schain, et al. (2006). "Interaction of human 15-lipoxygenase-1 with phosphatidylinositol bisphosphates results in increased enzyme activity." Biochim Biophys Acta 1761(12): 1498-505.
  • Guittard, G., A. Gerard, et al. (2009). "Cutting Edge: Dok-1 and Dok-2 Adaptor Molecules Are Regulated by Phosphatidylinositol 5-Phosphate Production in T Cells." J Immunol 182(7): 3974-3978.
  • Andersson, E., F. Schain, et al. (2006). "Interaction of human 15-lipoxygenase-1 with phosphatidylinositol bisphosphates results in increased enzyme activity." Biochim Biophys Acta 1761(12): 1498-505.
  • Krishnamurthy, K., S. Dasgupta, et al. (2007). "Development and characterization of a novel anti-ceramide antibody." J Lipid Res 48(4): 968-75.
  • Poon, I. K., M. D. Hulett, et al. (2010). "Histidine-rich glycoprotein is a novel plasma pattern recognition molecule that recruits IgG to facilitate necrotic cell clearance via FcgammaRI on phagocytes." Blood 115(12): 2473-82.