Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

Products

MobiSpin Column "F"

The Universal Spin Column for Centrifugation

MobiSpin Column F

MobiSpin column "F" can be closed with the tightly closing screw cap. Snap off the outlet plug and use its reverse side for closing.

Features

  • Comes empty - pack with your own material (matrix, gel slice etc.)
  • Easy handling: load sample, spin and collect the purified product
  • Fill in the matrix of your choice
  • Can be centrifuged in 1.5 ml or 2 ml tubes and fits all common centrifuges
  • Autoclavable at 110 °C for 10 min
  • No sample dilution
  • Reproducible results with fast protocols
  • Numerous samples can be processed simultaneously
  • Materials: polypropylene (column corpus), polyethylene (filters)
  • Free samples available

Description

  • The MobiSpin column "F" is designed for a wide variety of applications for nucleic acid and protein extraction and purification.
  • When choosing the MobiSpin column "F" for a particular application, a suitable resin must be selected and filled into the minicolumn. For an appropriate resin selection, impurity versus sample size and the anticipated yield must be considered.
  • The MobiSpin column "F" comes with inserted small or large filter of 10 µm pore size, screw cap and snapoff plug. It can be used without matrix (e.g. to extract trypsinated proteins out of a gel slice for Mass Spec analysis) or filled with a matrix of your choice.

Applications

For example you can use MobiSpin Columns "F" with gel filtration matrix for:

  • Removal of unicorporated dyes or dye terminators for purification of DNA after labeling reactions
  • DNA purification prior to sequencing
  • Removal of free nucleotides
  • Oligonucleotide purification after synthesis
  • Purification of RNA (Schneider et al., 2004)
  • Preparation of virus particles (Sachsenröder et al., 2012; personal correspondence with Nils Hartmann, Umweltbundesamt 2013)
MobiSpin F can be centrifuged

MobiSpin Columns "F" can be centrifuged in 1.5 ml or 2 ml tubes

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
M105010S MobiSpinColumn ""F"" with fixed outlet plug, inserted small 10 um filter and screw cap 50 100,00 PDF
M105210S MobiSpinColumn ""F"" with fixed outlet plug, inserted large 10 um filter and screw cap 50 100,00 PDF
  • All prices are in EURO excl. VAT and shipping. For further pricing and order information please ask your local distributor.

Related Products

The MobiSpin Columns "F" are also available pre-packed with:

  • Sephadex® G-50
  • Sephacryl® S-200, S-300 and S-400
  • or a compound of your choice on request.
Order# Description Amount Price Data Sheet
SCO200 MobiSpin S-200 20 columns 59,00 PDF
SCO210 MobiSpin S-200 100 columns 226,00 PDF
SCO234 MobiSpin S-200, S-300, S-400 3 X 10 columns 89,00 PDF
SCO300 MobiSpin S-300 20 columns 59,00 PDF
SCO310 MobiSpin S-300 100 columns 226,00 PDF
SCO400 MobiSpin S-400 20 columns 59,00 PDF
SCO410 MobiSpin S-400 100 columns 226,00 PDF
SCO500 MobiSpin G-50 20 columns 59,00 PDF
SCO510 MobiSpin G-50 100 columns 226,00 PDF
  • All prices are in EURO excl. VAT and shipping. For further pricing and order information please ask your local distributor.

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Literature

  • Sachsenröder J, Twardziok S, Hammerl JA, Janczyk P, Wrede P, Hertwig S, Johne R.: Simultaneous identification of DNA and RNA viruses present in pig faeces using process-controlled deep sequencing. PLoS One. 2012;7(4):e34631. doi: 10.1371/journal.pone.0034631. Pubmed
  • Schneider J, Buness A, Huber W, Volz J, Kioschis P, Hafner M, Poustka A, Sültmann H.: Systematic analysis of T7 RNA polymerase based in vitro linear RNA amplification for use in microarray experiments. BMC Genomics. 2004 Apr 30;5(1):29. Pubmed