Glycoconjugates
 
 
We have developed unique techniques for coupling oligosaccharides to lipids and proteins with the aim of creating powerful tools for use in a range of applications. These include:

Production of Monoclonal Antibodies

Using as little as 0.1 mg of neoglycoprotein per animal, is sufficient for the generation of antibodies to the required antigen. For this application, the short spacer arm version has been recommended by some of our customers.

While we offer both Bovine Serum Albumin and Human Serum Albumin conjugates as standard catalogue items, a relatively inexpensive custom service is available for conjugate preparation to others such as Tetanus Toxoid or Keyhole Limpet Haemocyanin (KLH).

Special prices are available for bulk amounts or pyrogen-free material, and you are invited to contact us to discuss your requirements.

Enzyme Acceptors

Experience has shown that the use of our unique 14 atom spacer arm when using neoglycoproteins as glycosyl transferase acceptors has proved to be advantageous. As shown below, the ability of 14 atom spaced 3'SLN-BSA to act as an acceptor for a fucosyl transferase was comparable to the Bovine Fetuin control. Using the shorter spaced version, was essentially ineffective.

We would therefore suggest that customers consider the steric influence of the protein prior to product selection.

ELISA

Many of our customers report that neoglycoproteins are ideal for ELISA. Please contact us for advice, or copies of technical bulIetins when ordering.

Quality Assurance

All of our neoglycoproteins are analysed by Matrix Assisted Laser Desorption Time Of Flight mass spectrometry (MALDITOF). This permits the determination of the number of covalently added molecules of antigen per molecule of carrier protein, together with the molecular weight distribution (maximum and minimum numbers of residues per molecule).

The traces obtained for three conjugates (NGPs 0334, 0501, and 0502) are illustrated below. The molecular weight for BSA is 66330 daltons. Comparing the degree of peak broadening of the neoglycoprotein relative to the BSA, gives an indication of the heterodispersity of the conjugate. Generally, neoglycoproteins have an average of 10 to 12 carbohydrates per molecule of protein, with a range of between 7 or 8 to 18 or 20.

Blood Group & Lewis Antigen Neoglycoproteins


 
 NGP0302
 
      Lewisx-BSA
 
0.25mg 0.5mg 1mg 5mg
 
 NGP0307
 
      2'Fucosyllactose (2'FL-BSA)
 
0.25mg 0.5mg 1mg 5mg
 
 NGP0501
 
      Lacto-N-fucopentaose II-BSA
 
Lewisa trisaccharide, but with an additional 'lactose' spacer
0.25mg 0.5mg 1mg 5mg
 
 NGP0502
 
      Lacto-N-fucopentaose III-BSA
 
Lewisx trisaccharide, but with an additional 'lactose' spacer
0.25mg 0.5mg 1mg 5mg
 
 NGP0503
 
      Lacto-N-fucopentaose I-BSA
 
Blood Group H (type I) linked b1-3 on to a lactose spacer
0.25mg 0.5mg 1mg 5mg
 
 NGP0601
 
      Lacto-N-difucopentaose I-BSA
 
The Lewisb antigen linked to a lactose spacer. Structure recognised by Helicobacter pylori in binding to human gastric epithelium (Borén et al (1993) Science 262 1892-4).
0.25mg 0.5mg 1mg 5mg
 
 NGP0305
 
      Blood Group A-BSA
 
Blood group A trisaccharide-BSA (3-atom spacer)
0.25mg 0.5mg 1mg 5mg
 
 NGP0323
 
      Blood Group B-BSA
 
Blood group B trisaccharide-BSA (3-atom spacer)
0.25mg 0.5mg 1mg 5mg
 
 NGP2340
 
      Globotriose-HSA (3-atom spacer)
 
Gala1-4Galb1-4Glc-HSA
0.25mg 0.5mg 1mg 5mg
 
Core Structure Neoglycoproteins
 

 
 NGP0201
 
      N-Acetyllactosamine-BSA
 
3-Atom Spacer
0.25mg 0.5mg 1mg 5mg
 
 NGP1201
 
      N-Acetyllactosamine-BSA
 
14 Atom Spacer
0.25mg 0.5mg 1mg 5mg
 
 NGP1336
 
      a1-3, a1-6 Mannotriose-BSA
 
14 Atom Spacer
0.25mg 0.5mg 1mg 5mg
 
Monosaccharide Neoglycoproteins
 
Only available with a 14-atom spacer
 
 NGP1101
 
      N-Acetylglucosamine-BSA
 
0.25mg 0.5mg 1mg 5mg
 
 NGP1104
 
      N-Acetylgalactosamine-BSA
 
0.25mg 0.5mg 1mg 5mg
 
Tumour Antigen Neoglycoproteins
 
For recent reviews: Hakamori, S. In: Serological Cancer Markers, Chapter 10, Sell, S., Ed; The Human Press: New Jersey 1992; Hakamori, S. (1991) Curr. Opin. Immunol. 3: 646
 
 NGP2208
 
      T-Antigen-HSA
 
Galb1-3GalNAc-HSA, 3-Atom Spacer
Carbohydrate disaccharide component of T-antigen or Thomsen Friedenreich antigen associated with tumor cells. Springer G.F (1984) Science 224: 1198
0.25mg 0.5mg 1mg 5mg
 
 NGP1103
 
      Tn-Antigen-HSA
 
GalNAca1-0-(Ser-N-Ac-CO)-Spacer-NH-HSA
The Tn antigen has been shown to be expressed in over 70% of lung, colon and stomach carcinomas. Itzkowitz et al, (1989) Cancer Res. 49: 197
0.25mg 0.5mg 1mg 5mg
 
Sialylated Neoglycoproteins
 
For the study of inflammation and cell adhesion processes involving Sialyl Lewisx and analogues
 
 NGP0301
 
      3'-Sialyl-N-Acetyllactosamine-BSA
 
3 Atom Spacer
0.25mg 0.5mg 1mg 5mg
 
 NGP1301
 
      3'-Sialyl-N-Acetyllactosamine-BSA
 
14 Atom Spacer
Will act as an acceptor for L-fucose with many fucosyl transferases to give a SLex neoglycoprotein
0.25mg 0.5mg 1mg 5mg
 
 NGP0403
 
      3'-Sialyl Lewisx-BSA
 
3-Atom Spacer
Caused inhibition of binding of carcinoembryonic antigen to E-Selectin (ELISA assay).
0.25mg 0.5mg 1mg 5mg
 
 NGP2403
 
      3'-Sialyl Lewisx-HSA
 
3-Atom Spacer
0.25mg 0.5mg 1mg 5mg
 
 NGP0405
 
      3'-Sialyl-3-Fucosyllactose-BSA
 
3-Atom Spacer
0.25mg 0.5mg 1mg
 
 NGP1403
 
      3'-Sialyl Lewisx -BSA
 
14-Atom Spacer
0.25mg 0.5mg 1mg
 
Gala1-3Gal Series Neoglycoproteins
 
For studying transplant rejection phenomena

We offer a series of neoglycoproteins with increasing chain lengths for the study of carbohydrate ligands containing alpha galactose. This range allows among other applications, the generation of monoclonal antibodies to these antigens, and the study of natural antibody populations with ELISA.


 
 NGP0203
 
      Gala1-3Gal-BSA
 
3-Atom Spacer
0.25mg 0.5mg 1mg 5mg
 
 NGP1203
 
      Gala1-3Gal-BSA
 
14-Atom Spacer
0.25mg 0.5mg 1mg 5mg
 
 NGP2203
 
      Gala1-3Gal-HSA
 
3-Atom Spacer
0.25mg 0.5mg 1mg 5mg
 
 NGP3203
 
      Gala1-3Gal-HSA
 
14-Atom Spacer
0.25mg 0.5mg 1mg 5mg
 
 NGP0334
 
      Gala1-3Galb1-4GlcNAc-BSA
 
3-Atom Spacer
0.25mg 0.5mg 1mg 5mg
 
 NGP1334
 
      Gala1-3Galb1-4GlcNAc-BSA
 
14-Atom Spacer
0.25mg 0.5mg 1mg 5mg
 
 NGP2334
 
      Gala1-3Galb1-4GlcNAc-HSA
 
3 Atom Spacer
0.25mg 0.5mg 1mg 5mg
 
 NGP3334
 
      Galá1-3Galâ1-4GlcNAc-HSA
 
14 atom spacer
0.25mg 0.5mg 1mg 5mg
 
 NGP0537
 
      Galili Pentasaccharide-BSA
 
3-Atom Spacer
0.25mg 1mg 5mg
 
Gala1-3Gal Analogue Neoglycoproteins
 
Versatile Tools (McKane et al (1998) Transplantation 66(5): 626-633)
 
 NGP0330
 
      Gala1-3Galb1-4Glc-BSA
 
3-Atom Spacer
Effective Ligand for IgG Anti-Gal Antibodies
0.25mg 0.5mg 1mg 5mg
 
 NGP2330
 
      Gala1-3Galb1-4Glc-HSA
 
3-Atom Spacer
Effective Ligand for IgG Anti-Gal Antibodies
0.25mg 0.5mg 1mg 5mg
 
 NGP0333
 
      Gala1-3Galb1-3GlcNAc-BSA
 
3-Atom Spacer
0.25mg 0.5mg 1mg 5mg
 
 NGP2333
 
      Gala1-3Galb1-3GlcNAc-HSA
 
3-Atom Spacer
0.25mg 0.5mg 1mg 5mg
 
 NGP2335
 
      Gala1-3Galb1-4(3-deoxyGlcNAc)-HSA
 
3-Atom Spacer
Specifically binds to IgM Anti-Gal Antibodies
0.25mg 0.5mg 1mg 5mg
 
 NGP2336
 
      Gala1-3Galb1-4(6-deoxyGlcNAc)-HSA
 
3-Atom Spacer
Specifically binds to IgM Anti-Gal Antibodies
0.25mg 0.5mg 1mg 5mg
 
Neoglycolipids
 
Glycolipids permit the adsorption of carbohydrate ligands onto plates, micro wells and a variety of other surfaces, so that the ligand may be used as a probe, in diagnostic tests and in ELISA.

We offer neoglycolipids which we have synthesised by conjugating oligosaccharides via an amino group and a 3-atom linker arm to 1,2-di-O-hexadecyl-sn-glycero-3-phosphoethanolamine (HDPE).

These products are sold as amorphous white solids. They can be reconstituted by dissolving with sonication in chloroform:methanol: water = 350:480:175.
 
 NGL0201
 
      Galb1-4GlcNAcb1-HDPE
 
C54H106N3PO17-FW 1099.73 0.25mg 0.5mg 1mg 5mg
 
 NGL0203
 
      Gala1-3Galb1-HDPE
 
C54H103N2PO17-FW 1058.70 0.25mg 0.5mg 1mg 5mg
 
 NGL0334
 
      Gala1-3Galb1-4GlcNAcb1-HDPE
 
C60H116N3PO22-FW 1262.73 0.25mg 0.5mg 1mg 5mg