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Mirus Corp. -
Gene Transfer Specialists

For more information please visit the Mirus webpage or download the Mirus catalog (1.8 MB pdf).
 

Below you will find some selected Mirus products.

 Mirus

button Label IT® Nucleic Acid Labeling Reagents
button Label IT® siRNA Tracker™ Intracellular Localization Kit
button Label IT® µArray™ Biotin Labeling Kit
button Label IT® µArray™ Dual Labeling Kit


Label IT® Nucleic Acid Labeling Reagents

Features and Advantages
  • one-step, non-radioactive labeling of nucleic acids using a single reagents
  • rapid and easy to perform
  • densities of 1 label per 10 bp are possible
  • covalently label the guanine residues

Suggested Applications

  • Southern, Northern, and dot (slot) blots, colony and plaque lifts, and in situ hybridizations
  • DNA and RNA tracking studies
  • DNase and RNase detection
  • DNA condensation assays

Product Description

The Label IT® Nucleic Acid Labeling Reagents offer efficient, one-step, nonradioactive labeling of DNA or RNA using a single reagent.The nonenzymatic Label IT® Reagents covalently label the guanine residues of the nucleic acid in a non-destructive manner.The labeling reactions are easy to perform, rapid, and can be scaled up without decreasing the labeling efficiency. Adjusting the incubation time and/or the amount of reagent easily controls the labeling density. The Label IT® Reagents efficiently label any nucleic acid - DNA (doublestranded, single-stranded, super coiled and linear) and RNA. Simply adjusting the ratio of reagent to nucleic acid or the reaction time can easily control the extent of modification; densities of one label per 10 bp are possible.
Fluorescence In Situ Hybridization
Fluorescence In Situ Hybridization : Human lymphocyte metaphase spreads hybridized with a Label IT® Cy™3 labeled chromosome 20 PAINT probe.

Labeling of a Synthetic Oligonucleotide.
Labeling of a Synthetic Oligonucleotide. A 18-mer oligonucleotide was labeled in a standard reaction using the Label IT® CX-Rhodamine Reagent.

ORDER INFORMATION:
order# description amount
MIR 3125 Label IT® CX-Rhodamine Labeling Kit labels 25 µg nucleic acid
MIR 3100 Label IT® CX-Rhodamine Labeling Kit labels 100 µg nucleic acid
MIR 3225 Label IT® Fluorescein Labeling Kit labels 25 µg nucleic acid
MIR 3200 Label IT® Fluorescein Labeling Kit labels 100 µg nucleic acid
MIR 3325 Label IT® Digoxin Labeling Kit labels 25 µg nucleic acid
MIR 3300 Label IT® Digoxin Labeling Kit labels 100 µg nucleic acid
MIR 3425 Label IT® Biotin Labeling Kit labels 25 µg nucleic acid
MIR 3400 Label IT® Biotin Labeling Kit labels 100 µg nucleic acid
MIR 3625 Label IT® Cy™3 Labeling Kit labels 25 µg nucleic acid
MIR 3600 Label IT® Cy™3 Labeling Kit labels 100 µg nucleic acid
MIR 3725 Label IT® Cy™5 Labeling Kit labels 25 µg nucleic acid
MIR 3700 Label IT® Cy™5 Labeling Kit labels 100 µg nucleic acid
MIR 3825 Label IT® DNP Labeling Kit labels 25 µg nucleic acid
MIR 3800 Label IT® DNP Labeling Kit labels 100 µg nucleic acid
MIR 4125 Label IT® TM-Rhodamine Labeling Kit labels 25 µg nucleic acid
MIR 4100 Label IT® TM-Rhodamine Labeling Kit labels 100 µg nucleic acid
store all components at -20 °C  

Download: Label IT ®product information

See also Chapter 7, pages 128 of our online catalog.


Label IT® siRNA Tracker™ Intracellular Localization Kit
Features and Advantages
  • label, transfect and track the siRNA of your choice
  • easy one-step covalent attachment with a variety of labels enabling multi-color tracking: Cy™3, Cy™5, CX-Rhodamine, TM-Rhodamine, Fluorescein and Biotin
  • high efficiency siRNA delivery using Trans IT®-TKO™ Transfection Reagent
  • real time tracking of siRNA delivery


Product Description

The Label IT® siRNA Tracker™ Intracellular Localization Kit provides a straight forward approach to directly label and deliver siRNA, of your design, in an efficient yet non-destructive manner, for in vitro tracking experiments. Subcellular localization and functional inhibition of target gene expression can be monitored following introduction of the labeled siRNA into mammalian cells. This kit provides sufficient reagents to label 50 µg of siRNA and perform at least 500 transfections in 24-well plates. Trans IT®-TKO™ Transfection Reagent, which is included in the kit, is designed specifically for high efficiency, low toxicity siRNA transfections in a variety of cell lines.
C5 Cells transfected with Label IT® siRNA Tracke

C5 Cells transfected with Label IT® siRNA Tracker™ Cy™3 labeled siRNA and Trans IT®-TKO™ Transfection Reagent in complete media for 24 hours.

ORDER INFORMATION:
order# description amount
MIR 7200 Label IT® siRNA Tracker™ Cy™3 labels 50 µg siRNA
MIR 7201 Label IT® siRNA Tracker™ Cy™5 labels 50 µg siRNA
MIR 7205 Label IT® siRNA Tracker™ Fluorescein labels 50 µg siRNA
MIR 7202 Label IT® siRNA Tracker™ CX-Rhodamine labels 50 µg siRNA
MIR 7203 Label IT® siRNA Tracker™ TM-Rhodamine labels 50 µg siRNA
MIR 7204 Label IT® siRNA Tracker™ Biotin labels 50 µg siRNA
store components at 4°C

Download: Label IT® siRNA Tracker product information

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Label IT® µArray™ Biotin Labeling Kit

The Label IT® µArray™ Biotin Labeling Kit is recommended for single-channel microarray applications.

Features and Advantages
  • Directly label and hybridize mRNA samples to microarrays.
  • Simple, one-step labeling reaction for any nucleic acid of any length.
  • Covalent labeling chemistry eliminates modified nucleotides and enzymatic incorporation biases.
  • Optimized labeling for robust performance on microarrays.
  • Complete protocol for labeling mRNA, cDNA, and cRNA samples is included.


The Label IT® µArray™ Biotin Labeling Kit is recommended for single-channel microarray applications. The use of biotin as a marker molecule for hybridization requires a post-hybridization detection procedure with a fluorophore. As such, a biotin-labeled sample provides the flexibility to use any of a variety of established single color detection strategies. In single-color microarray experiments, one sample is hybridized and detected per microarray slide and relative gene expression can be determined from comparison of independently normalized data sets for each sample.

The Label IT® µArrayx Biotin reagent labels any nucleic acid sample, e.g. cRNA on duplicate oligo arrays
The Label IT® µArray™ Biotin reagent labels any nucleic acid sample, e.g. cRNA on duplicate oligo arrays

ORDER INFORMATION:
order# description amount
MIR 8010 Label IT® µArray™ Biotin Labeling Kit 10 reactions
MIR 8050 Label IT® µArray™ Biotin Labeling Kit 50 reactions
store label reagent at -20°C, the other components at 4°C

Download: Label IT® µArray™ Biotin Labeling Kit product information

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Label IT® µArray™ Dual Labeling Kit

The Label IT® µArray™ Dual Labeling Kit is recommended for dual-channel microarray applications.

  • Directly label and hybridize mRNA samples to microarrays.
  • Simple, one-step labeling reaction for any nucleic acid of any length.
  • Covalent labeling chemistry eliminates modified nucleotides and enzymatic incorporation biases.
  • Optimized labeling for robust performance on microarrays.
  • Complete protocol for labeling mRNA, cDNA, and cRNA samples is included.

The Label IT® µArray™ Dual Labeling Kit is recommended for dual-channel microarray applications and includes Label IT® µArray™ Reagents for labeling samples with biotin and fluorescein. The use of biotin and fluorescein as marker molecules for hybridization requires a post-hybridization detection procedure with different fluorophore-labeled conjugates. As such, biotin- and fluorescein-labeled samples provide flexibility and compatibility with a variety of established dual color detection strategies. A simple detection method using Cy™3-conjugated streptavidin and a Cy™5-conjugated anti-fluorescein antibody that is compatible with standard microarray scanners is provided in the kit protocol.

The Label IT® µArrayx Dual Kit labels any nucleic acid sample, e.g. mRNA on duplicate cDNA arrays
The Label IT® µArray™ Dual Kit labels any nucleic acid sample, e.g. mRNA on duplicate cDNA arrays


ORDER INFORMATION:
order# description amount
MIR 8105 Label IT® µArray™ Dual Labeling Kit (Biotin and Fluorescein reactions, 5 labelings each) 10 reactions
MIR 8125 Label IT® µArray™ Dual Labeling Kit (Biotin and Fluorescein reactions, 25 labelings each) 50 reactions
store label reagent at -20°C, the other components at 4°C

Download: Label IT® µArray™ Dual Labeling Kit product information

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