products | AMRESCO, Inc.

AMRESCO Inc.

AMRESCO is a manufacturer & supplier of high-quality biochemicals and reagents for Molecular Biology, Life Sciences, Clinical, and Histology areas of research. Founded in 1976, AMRESCO supplies standard and custom products for customers in the following industries: biotechnology, biopharmaceutical, diagnostics, research specialty chemicals, and related areas.

For more information please visit the webpage.

Following you will find some selected AMRESCO-products:

Investigator and Catalog:

Next Gel

Imagine an electrophoresis system that enables you to get publication quality gels the first time…. every time

• Resolve 14.2 kDa band from a 14.4 kDa band on a mini-gel
• Separate 3.5 kDa and 212 kDa proteins on the same gel
• No stacking gel required
• Pour an acrylamide gel in 1 minute
• Room temperature stability for up to 1 year

NEXT GEL™* is a revolutionary ready-to-pour premixed solution of acrylamide, bisacrylamide, gel buffer and SDS that enables ultra-fine resolution of protein bands superior to other commercially available products.
The unique acrylamide matrix of NEXT GEL™ slows the progression of proteins, eliminating the need for a stacking gel. This not only saves time, but also permits the proteins to run across a longer gel surface resulting in increased resolution. The proprietary chemistry of NEXT GEL™ has gradient-like properties, which enables the separation of small peptides and high molecular weight proteins in the same gel. NEXT GEL™ has been formulated to obtain batch-to-batch consistency, reliable and improved protein separation for critical analysis. This system is fully compatible with standard SDS-PAGE applications such as 1D & 2D gels, Western blot transfer, protein sequencing, MALDI analysis and common stain methods.

*NEXT GEL™ products are patent pending

Also available:

NEXT GEL ^TM for Non-Denaturing Protein Work (#M271-KIT),
LP-NEXT GEL^TM Kit ideally suited for large proteins (#M272-KIT),
HTS NEXT GEL^TM Kit for processing up to 200 samples in 2 hours (Code: M271-KIT)

Destaining Bags

AMRESCO destaining bags efficiently remove Ethidium Bromide, Coomassie Blue, and other biological dyes from solution. The special adsorbent mixture retains dye molecules in a convenient bag for safe removal and incineration. Each bag will extract up to 5 mg of Ethidium Bromide from solution during an overnight treatment.

How to use AMRESCO’s Destaining Bags

The Destaining Bags from AMRESCO are an acid/base mixed bed of resin, which are expected to absorb 1-5 mg of ethidium bromide from gel solutions and running buffers after gentle overnight agitation. The intent of these bags is to remove hazardous dyes like ethidium bromide from gel solutions for easy disposal. For example, if you have a 200 ml solution with 2 g/L of ethidium bromide, the solution contains 200 mg of dye. To remove all the dye from the solution would require (200/5 =) 40 Destaining Bags. If the intent is to remove Coomassie from a solution, there are several issues that need to be understood first. The Coomassie molecule is over 2 times as large as the ethidium bromide molecule, which means dye removal will not be as effective as with ethidium bromide removal. Also, Coomassie R-250 is dissolved in an acetic acid solvent. When the Destaining Bags are placed into this solution the resin becomes saturated with the acetic acid, resulting in slower and not as effective removal of the dye. Concentration removal is claimed only for ethidium bromide removal from solution. Use for removal of other dyes may require less or more bags and time for complete treatment.

SYBR^® Green I

• Superior Sensitivity (20 pg) for Detecting Nucleic Acids
• No Background Fluorescence for Fast and Efficient Staining
• Compatible with In-Gel Digestion and Ligation Techniques

SYBR^® Green I Nucleic Acid Stain is one of the most sensitive stains available for detecting DNA in agarose and polyacrylamide gels. Less than 20 pg of double-stranded DNA can be detected in a single band using 254 nm epi-illumination, black and white Polaroid® 667 print film, and SYBR^® Green gel stain photographic filter. This sensitivity is 25 times greater than staining achieved using ethidium bromide under standard 312 nm transillumination. SYBR^® Green I stain does not inhibit the activity of many common restriction endonucleases1,2 making it compatible with in-gel digestions and ligation techniques. Thus, the removal of SYBR^® Green I stain is not necessary when sub-cloning stained DNA fragments isolated from gels.

SYBR^® Green II

• Excellent Sensitivity (100 pg) for Detecting RNA and ssDNA
  
• No Background Fluorescence for Fast and Efficient Staining
  
• Compatible with Hybridization and Cycle-Sequencing Reactions
  

SYBR^® Green II RNA gel stain is an exceptionally sensitive dye for detecting RNA in electrophoretic gels. As little as 100 pg RNA or single-stranded DNA per band can be detected in a SYBR^® Green II dye-stained agarose or polyacrylamide gel using 254 nm epiillumination, black and white Polaroid 667 print film and a SYBR^® Green gel stain photographic filter.3,4 Even with 300 nm transillumination, as little as 500 pg RNA per band can be detected. In agarose/formaldehyde gels with SYBR^® Green ll RNA gel stain does not interfere with transfer of RNA to filters or subsequent hybridization in Northern blot analysis as long as 0.1% - 0.3% SDS is included in prehybridization and hybridization buffers. SYBRC Green II stain is also compatible with amplification by PCR after SSCP analysis, SYBR^® Green II stained bands can be excised out of the gel and used in cycle-sequencing.

For a detailed data sheet as a pdf-file, please click on the product No.

ORDER INFORMATION:
order#	description	amount
J976-500ULAM	SYBR® Green I Nucleic Acid Stain	500 µl
K143-500ULAM	SYBR® Green II RNA Stain Gel	500 µl
J975-1FILTERAM	SYBR® Green Gel Stain Photographic Filter	1

SYBR is registered trademark of Molecular Probes

PAGE-PLUS^TM

Superior Acrylamide for DNA Sequencing & Nucleic Acid Separations

Features and Advantages

• Superior Gel Matrix for DNA Sequencing and Nucleic Acid Separations
  
• Provides Longer Reads and Greater Accuracy
  
• Safe and Convenient Blend for Consistent Results
  

AMRESCO's commitment to excellence has led to the development of PAGE-PLUS^TM , a superior polyacrylamide matrix that outperforms standard acrylamide/bis-acrylamide blends.

PAGE-PLUS^TM is a high resolution matrix specifically designed for superb resolution of DNA sequencing products and low molecular weight DNA. This blend of specially modified acrylamides and proprietary cross-linkers enables researchers to gather up to 40% more readable sequence information per sequencing gel. The unique matrix of PAGE-PLUS^TM gels leads to improved resolution, consistent band spacing, and faster running time over standard bis-acrylamide cross-linked gels. This novel matrix also exhibits increased mechanical strength to resist tearing during post-electrophoresis manipulations. PAGE-PLUS^TM is available in a convenient 40% concentrated solution (PAGE-PLUS^TM Concentrate) and ready-to-use concentrations specifically designed for DNA sequencing and other denaturing electrophoresis applications (Gene-PAGE PLUS^TM ). In addition to the exceptional resolving power of the PAGE-PLUS^TM matrix, all solutions save valuable time while minimizing exposure to hazardous powdered acrylamide. PAGE-PLUS^TM Concentrate is a 40% solution of modified acrylamides ideal for preparing custom gels of nearly any concentration. This concentrate is stable at room temperature for up to 1 year. PAGE-PLUS^TM has been proven effective for use on a number of different gel sequencing platforms. PAGE-PLUS^TM can be optimized for specific run conditions and platforms by adjusting acrylamide as well as urea and TBE concentrations. See AMRESCO's online catalog on page 113 for additional recommendations for sequencing with PAGE-PLUS^TM products.

For a detailed data sheet as a pdf-file, please click on the product No.

For more information please visit AMRESCO's web-page
or view the online catalog!

Agarose I^TM Sample Kit

AMRESCO's Agarose I^TM Sample Kit is a perfect and convenient way for molecular biology labs to try several of our outstanding electrophoresis products at a very low price!

This specially packaged kit contains sample sizes of AMRESCO's:

• Agarose I^TM
• TBE Buffer
• 6X Agarose Gel Loading Dye
• Destaining Bags

Includes necessary components to run between 3 and 6 mini-gels.

Our high-quality Agarose I^TM is ideal for general applications. AMRESCO's Destaining Bags provide a simple way to remove ethidium bromide from solution. The 6 X Agarose Gel Dye completes the kit as a unique 3-in-1 loading dye that is extremely accurate in migration estimations.

For more information please visit AMRESCO's web-page or view the online catalog!

Glycoprotein Staining Kit

Features and Advantages

• Sensitive glycoprotein detection
  
• Quick protocol
• Convenient
• Easy-to-use

AMRESCO's Glycoprotein Staining Kit has recently been developed for the Pro-Pure^TM proteomics line for the detection and staining of glycoproteins on SDS-PAGE gels. This 3-component kit is sufficient to stain 8 gels, providing a low background for each and every one. Perfect for researchers, the Glycoprotein Staining Kit has a quick protocol, is convenient and easy to use, and - most importantly - provides sensitive glycoprotein detection.

For more information please visit AMRESCO's web-page or view the online catalog!

The LiGator^TM Kit

• Increased productivity and efficiency
  
• Complete ligation reactions in minutes
  
• Efficient cloning at low concentrations of DNA
  

The LiGator^TM Kit allows ligation reactions to be completed in just 15 minutes - much faster than the usual overnight protocol. This advanced method allows the user to purify, ligate, and transform all in one day. Positive colonies containing the construct of interest are now achieved overnight rather than in several days. The LiGator^TM Kit significantly reduces reaction time because it incorporates the theory of molecular crowding which not only speeds up the reaction, but also allows for more efficient cloning when using low concentrations of DNA. Additionally, by altering the distribution of ligation products, intermolecular ligation is minimized.

The LiGator^TM Kit is made up of a T4 DNA Ligase (4U/µl), 10X Reaction Buffer, Nuclease-Free Water, & Agarose 3:1 HRB^TM, and contains enough reagents to perform 30 ligation reactions.

For more information please visit AMRESCO's web-page or view the online catalog!

HistoChoice^® MB

Non-Formalin based Tissue Fixative

Features and Advantages

• Contains no formaldehyde, mercury, or glutaraldehyde
• Odorless and non-toxic
• Can be safely washed down the drain upon neutralization (in most districts)
• Superior sensitivity without predigestion
• Vibrant staining with sharp visual intracellular detail
• Full nuclear and cellular detail preserved
• No crosslinking problem of tissues as with Formalin-based fixatives

AMRESCO's HistoChoice^® Molecular Biology (MB) Fixative is a safe, convenient solution for tissue fixation developed specifically for the molecular biologist. HistoChoice^® MB Fixative improves healthcare delivery because it is safe to use and is gentle on tissues and the environment. HistoChoice^® MB directly replaces common fixatives including: formaldehyde- and alcohol-based fixatives, Zenker' s, B5 & B3, Bouin' s and other fixatives with superior results.

For more information please visit AMRESCO's web-page
or view the online catalog!

Tracking Dyes

AMRESCO offers a variety of general purpose tracking dyes for DNA, RNA, and protein work. Bromocresol Green is generally used for RNA gels. Bromophenol Blue is suitable for nucleic acid & protein gels. Both the Bromocresol Green and the Bromphenol Blue dyes are also available in sodium salt forms. Xylene Cyanol is used with DNA gels. AMRESCO's 2D Tracking Dye is premixed, ready-to-use for protein gels. This tracking dye accurately monitors the progress of the 2nd dimension gel during 2D electrophoresis.

Standard Detergents

Please find more standard detergents and high-purity detergents for proteomics use in AMRESCO's catalog or on AMRESCO's homepage!

Loading Dyes

Loading dyes are designed to optimize loading DNA, RNA, and proteins in acrylamide and agarose gels. Loading dyes serve three functions in electrophoresis. First, dyes impart color to the sample to facilitate the loading process. Second, loading dyes increase the density of the sample to ensure efficient sample distribution into each well. Third, the dyes themselves migrate independently from the samples, allowing the user to estimate the migration of nucleic acids or proteins.

All AMRESCO loading dyes feature our exclusive blend of three tracking dyes that make estimating sample migration simple and reliable. Dye #1 - A light blue dye that migrates at 4,000 base pairs in 1% agarose. Dye #2 - An indigo dye that migrates at about 600 base pairs in 1% agarose. Dye #3 - A magenta dye that migrates at 150 base pairs in 1% agarose.

Ethidium Bromide

A widely used fluorescent stain for visualizing nucleic acids in solution or electrophoresis gels. At a concentration of 0.5 µg/ml, it is useful in agarose or acrylamide gels for band detection of as little as 10 ng of nucleic acid at 312 nm. At higher concentrations (0.5 - 1.0 µg/ml), Ethidium Bromide may be used to facilitate purification of DNA in a cesium chloride gradient. For maximum convenience to each end user, AMRESCO offers Ethidium Bromide in various forms. Each is packaged in light-proof, shatter-resistant packaging for added safety.

For more information on AMRESCO products please visit AMRESCO's web-page or view the online catalog!