MoBiTec - Product Highlights - NEWS

New Endoproteases Brochure available - Including MobiTEV protease

The new brochure includes our expanding collection of Endoproteases:

• MobiTEV Protease - Improved TEV Protease at an Affordable Price
  
• HRV3C Protease - Highly Active Protease for the 'PreScission' Site
  
• IgA Protease - Especially Usefull for Cleavage of Fusion Protein in Inclusion Bodies
  
• Factor Xa Protease - The Endoprotease that Creates Authentic Recombinant Proteins
  
• Kex2 Protease - Protease for Alternative Cleavage Sites
  
• Pro39 Protease - Very Specific and Tuneable Protease

Download the new Endoprotease Brochure as PDF (460 kb) or order a free paper Copy!

EosFP - Green to Red Photoconvertible Fluorescent Protein for Local Marking

New brochure on Molecular Screening Technology available from MoBiTec

This 16-page brochure focuses on several yeast one- and two-hybrid systems and a phagemid system, that are useful for high-throughput molecular screening of protein-protein and DNA-protein interactions. Further tools, such as biochemical assays, immunological and fluorescence-based methods, are available from MoBiTec as well. The yeast two-hybrid system has rapidly become one of the most widely used techniques in molecular biology. It still is the method of choice to identify protein-protein interactions from either cDNA libraries or known gene sequences in vivo. The method relies on the transactivation of reporter genes in Saccharomyces cerevisiae identifying positive interactions.

Download the new Molecular Screening Brochure (1.2 MB) or order a free paper Copy!

Content:

Grow’n’Glow GFP Reporter Systems

MoBiTec’s Grow’n’Glow one- and two- hybrid systems are powerful tools for gene discovery and functional analysis. A GFP reporter allows one-step selection of positive clones under UV light.

• Grow’n’Glow GFP Two-Hybrid System
  Time-saving identification of positive clones with the well-established GFP reporter system, suitable for high-throughput screening assays. See page 2-3.
  
• Grow’n’Glow GFP One-Hybrid System
  Ideal to isolate genes of novel DNA-binding proteins or to map residues/motifs responsible for DNA-binding.
• Grow’n’Glow ACE1 Two-Hybrid System
  Stringent selection and more efficient detection of protein interactions using the copper-inducible CUP1/ACE1 system.

Y2H Membrane Systems

MoBiTec’s split-ubiquitin based systems circumvent the restrictions that are usually associated with traditional Y2H systems, now allowing to detect interactors with integral membrane proteins, self-activating and transcriptionally active proteins.

• Y2H Membrane System
  Versatile, reliable detection and analysis of specific binding partners of full-length integral membrane and membrane-associated proteins.
  
• Y2H Transactivating Protein System
  Screens transcriptionally active and self-activating proteins that cannot be screened using classical Y2H systems.

Tools and Media

MoBiTec offers many tools optimized for working with Y2H and yeast: Kits to transform yeast cells, kits to purify plasmids from yeast, media, supplements and fermentation technology.

• Yeast Transformation & Yeast Plasmid Purification Kits
• Yeast Media

Phagemid System

• Phagemid Display System
  Easy-to-handle, fast and low-cost approach for highthroughput and high-content screening of protein-protein interactions.

Download the new Molecular Screening Brochure (1.2 MB) or order a free paper Copy!

BioProbes 56 available

Get the latest information on cell biology products from Molecular Probes and their applications. Download the entire BioProbes 56 newsletter (PDF, 6.82 MB) or view individual articles below.

• COVER STORY:
• say goodbye to fluorescein
  
  
• NEW TECHNOLOGIES:
• Tools for studying dynamic cellular processes
• The next generation in protein profiling
• Measuring mitochondrial protein levels and enzyme activity
  
  
• PRACTICAL APPLICATIONS
• Seeing clearly: Accurate cell identification
• Evaluating cellular viability on human primary cells
• Putting regulatory T cells to work
• IgG antibody labeling from A to Z
• A cell-based approach for interrogating the PI3K/AKT/mTOR pathway
• Analyzing signaling events with phosphorylation site–specific antibodies
• What your colleagues are doing with the Qubit® quantitation platform
  
  
• JOURNAL HIGHLIGHT
• Recent news in neurodegeneration
  
  
• JUST RELEASED
• new thiol-reactive probe, assays for the JAK/STAT pathway, and more
  
  
• ENDNOTE
• applications in signal transduction, high-throughput screening, virology, and more

New Partner Company - LifeSpan

MoBiTec is proud to announce the appointment of a leading antibody manufacturer in molecular pathology - LifeSpan Biosciences. LifeSpan offers one the industry's largest inventory of rabbit polyclonal anti-human peptide antibodies to gene families including the G Protein-Coupled Receptors (GPCRs), Nuclear Receptors (NRs), and Kinases. LifeSpan's antibodies have been especially optimized for immunohistochemistry applications in human tissues.

Please use our product search for the product range of more than 30'000 antibodies from LifeSpan.

About LifeSpan BioSciences, Inc.

LifeSpan BioSciences, headquartered in Seattle, Washington, is a privately held company founded in 1995 that employs human tissues, specific antibodies and immunohistochemistry to identify and validate targets for its biotechnology and pharmaceutical company customers in Europe, Asia and North America. Products and services include more than 1,300 antibodies, custom IHC-validated antibody synthesis, IHC contract research services, custom tissue microarray (TMA) construction for use in contract research studies, pathology and imaging services, monoclonal antibody cross-reactivity screening services, subscription access to comprehensive human tissue IHC localization profiles for 400+ Drug Targets, an expression focused informatics resource for 3,200 genes.

FRET brochure from AnaSpec

The new FRET brochure from AnaSpec contains:

• Biological Application of FRET - Protease Detection
• FRET Quenchers and Donors
• ACE2 (Angiotensin Converting Enzyme) Assay Kits
• HCV (Hepatitis C Virus) Assay Kits
• HIV (Human Immunodeficiency Virus) Assay Kits
• MMP (Matrix Metalloproteinasen) Assay Kits
• Renin Assay Kits
• TACE (TNF-α Converting Enzyme) or α-Secretase Assay Kit
• β-Secretase Assay Kit
• WNV (West Nile Virus) Assay Kit
• Other FRET Substrates
• FRET Building Blocks
• Guidelines in Designing FRET Peptide Substrates

New NEXT GEL brochure from Amresco

MBL Life Science Catalog 2008 available!

The new MBL catalog 2008 with more than 5500 products and antibodies is now available. It includes the following chapters:

• Apoptosis
• Cell Proliferation
• Cytokines & Growth Factors
• GPCR
• Cell Surface Antigens
• Signal Transduction
• Cellular Stress
• Neuroscience
• Epitope Tags
• Allergy & Immunology
• Fluorescent Proteins
• Molecular Biology
• Genetics
• Autophagy & Proteolysis
• Virology

Order your paper copy today! Please contact us.

New Mirus RNA interference Brochure available

The new Mirus RNAi Tools Broschure is now available. Content:

• siRNA and miRNA Transfection
• shRNA/siRNA Expression Vector Transfection
• In Vivo Delivery of siRNA and/or shRNA/siRNA Expression Vector
• Delivery, Localization and Functual Knockdown Assessment
• Delivery and Localization Assessment
• miRNA Expression Profiling

Download the new RNAi Brochure as PDF (835 kb) or order a free paper Copy!

New Member of the CoralHue® Family: Monomeric Kusabira-Orange (KO)

The fluorescent protein CoralHue® Kusabira-Orange (KO) has been cloned from the stony coral Fungia concinna, whose Japanese name is “Kusabira-ishi”. The new mKO2 has been carefully engineered to form a monomer and thus has the feature of rapid maturation while retaining the features of the wild-type KO of brilliance and pH stability. CoralHue® mKO2 can be used to label proteins or subcellular structures, or for reporter assays.
Application: Monomeric Kusabira-Orange 2 was involved in constructing a cell cycle indicator coined „Fucci“ which allows to label G1 phase red and S/G2/M phase green. See a video showing the spectacular cell cycle visualization.
(Reference: Sakaue-Sawano et al. (2008) Cell 132, 487-498 Pubmed ).

Features

• Brilliant fluorescence & superior quantum yields
• Reduced pH sensivity
• Rapid maturation
• Monomeric protein eliminates aggregation problems

Several versions of KO2 plasmids are available from MoBiTec, e.g. CoralHue^® phmKO2-MCLinker that is codon-optimized for higher expression in mammalian cells and has a flexible linker between the fluorescence protein and the multicloning site.

Ref.
Sakaue-Sawano et al.: Visualizing spatiotemporal dynamics of multicellular cell-cycle progression.
Cell. 2008 Feb 8;132(3):487-98. Pubmed

New Acella™ Competent E. coli Cells

The newest innovation from Edge BioSystems, Acella™ chemically competent and electrocompetent cells enable cloning and expression in the same cell. These cells combine the characteristics of a superior cloning strain with the elements of a regulated protein expression system, eliminating time-consuming multiple transformations - so you can go from ligation to expression faster than ever.

Reade more
Download the Acella™ Brochure (PDF, 1.4 MB)

New Amresco Investigator 01-2008

The new Amresco Investigator including

• Cell Culture - animal free reagents
• Reagents for Cell Culture - Bacterial Freezing Media
• Stripping Buffers for Western Blots
• Yeast Media
• Nucleic Acid Analysis - PCR Kits & Ready-to-use DNA Ladders

Download the new Investigator as PDF or ask for a free paper Copy!

Channel Blocker Peptides

Peptide toxins are potent and selective compounds that can act on ion channels in plasma membrane of excitable cells.1 These high specificity and affinity compounds, often with diverse and selective pharmacologies, are used as pharmacological probes to study and characterize various receptor proteins involved in ion-channel function.1 These toxin peptides generally consist of a relatively small number of structural frameworks that are particularly suited to addressing the crucial issues of potency and stability.1 Multiple disulfide bonds constitute essential structural elements in many of these bioactive peptides, which are generated by proteolytic processing of prefolded protein precursors and then released from the cells in the bioactive form into the extracellular medium to exert their physiological function.

Request AnaSpec’s Peptides catalog today. Please find follwing a list of channel blocking peptids:

Reference: 1. Lewis, RJ. and ML. Garcia, Nat. Rev. Drug Discov. 2, 790 (2003).

BioProbes 54 available

How much time and effort would you save if one label worked for multiple detection methods? With TC-FlAsH™ TC-ReAsH™ tetracysteine (TC) reagents, you don't have to wonder anymore.

Articles in NEW TECHNOLOGIES describe our Click-iT™ EdU cell proliferation assays for multiple platforms, new PLA1- and PLA2-specific substrates, and more.

Articles in PRACTICAL APPLICATIONS give you in-depth product information, including new data describing the use of quantum dots as replacements for tandem dyes in flow cytometry; quantitating site-specific protein phosphorylation using phosphoELISA™ assays; an overview of fluorescent calcium indicators for in vivo imaging of neuronal networks; and others.

Download your digital BioProbes 54 (PDF, 2.8 MB)

World's First Fluorescent Protein-Protein Interaction Detection Kit!

CoralHue® Fluo-chase Kit is the world's first fluorescent protein kit, which enables the visualization of protein-protein interactions as fluorescent signals in mammal cells within 24 hours. The CoralHue® Fluo-chase Kit allows for a high sensitivity analysis, especially in weak protein-protein interactions. Unlike any other bimolecular fluorescent You will be able to see the protein interaction in vivo state.

CoralHue® Fluo-chase Kit Flyer (PDF, 660 kb)

CoralHue® Fluo-chase Kit Handbook (26 Pages, PDF, 1.2 MB)

MoBiTec wishes all customers Merry Christmas
and a happy & prosperous new year!

Fluorophore Selection Guide

Get a Free Copy of our Popular Fluorescence Poster (size Din A1) with any Order in the Category 'Fluorescence Technology'

Of course you can order an example individually for 15 Euro or download the Fluorescence Poster as pdf (size Din A4, 186 kb)

New 'CoralHue® Fluorescent Proteins' Brochure

New brochure from MBL International with fluorescent proteins for live-cell imaging available.

• CoralHue® Keima-Red Large Stokes Shift Fluorescent Proteins
  
  
• CoralHue® Kaede and Kikume Green-Red Photoconverting Fluorescent Proteins
  
  
• CoralHue® Dronpa Photochromic Fluorescent Protein
  
  
• CoralHue® Kusabira-Orange, Azami Green, Midoriishi Cyan Fluorescent Proteins

Download the new MBL CoralHue® Fluorescent Proteins Brochure today (PDF, 2 MB)!
Please contact us for a free paper copy.

Updated 'Kinase Assay Kits' Brochure

Download the new MBL Kinase Assay Kits Brochure today (PDF, 830 kb)!
Please contact us for a free paper copy.

EZ-VISION™ - Non-Toxic DNA Staining

EZ-VISION™ is a fluorescent DNA stain that enables instant band visualization during or immediately after running your agarose gel.

EZ-VISION™ forms a tight complex with the sample DNA and co-migrates with it during electrophoresis. DNA bands can be visualized immediately after the run by placing the gel on a standard UV transilluminator. Post-run staining and destaining is completely eliminated. Sensitivity is comparable to Ethidium Bromide.
EZ-VISION™ is non-hazardous and eliminates special shipping, handling and disposal considerations. It is ideal for applications needing rapid DNA band visualization and for environments requiring a safe, non-hazardous alternative to Ethidium Bromide.

Features:

• Fast - Visualize DNA bands instantly after electrophoresis. No staining or destaining needed
• Safe - Reduce the use of Ethidium Bromide
• Environmentally Friendly - No hazardous chemicals that require special handling or disposal
• Cost Effective - No hazardous shipping charges

Read more about EZ-VISION™ DNA stain

News Archiv

for older news please look at our news archiv:

• 2007
• 2006
• 2005