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Special Offer | MMPs |
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Matrix metalloproteinases (MMPs) -
Special Offer 20% off*
*Valid through Mar 31, 2007. Please refer to order code MTJFM for getting the discount. |
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Introduction
Matrix metalloproteinases (MMPs) are a family of highly homologous protein-degrading zinc dependent endopeptidases.This family currently includes more than 25 members that can be divided into collagenases (MMP-1, -8, and -13), gelatinases (MMP-2 and -9), stromelysins (MMP-3 and -10), matrilysins (MMP-7 and -26), and the membrane-type MMPs (MMP-14 to -17 and -24). MMPs are important in many normal biological processes including embryonic development, angiogenesis, and wound healing, as well as in pathological processes such as inflammation, cancer, and tissue destruction. MMPs collectively cleave most, if not all, of the constituents of the extracellular matrix (ECM) and are involved in the breakdown and remodeling of many tissues and organs.
MoBiTec introduces a range of products manufactured by AnaSpec for the next level in MMP detection. EnzoLyte™ fluorimetric MMP assay kits use proprietary FRET (Fluorescence Resonance Energy Transfer) substrates to accurately detect activity in a wide range of MMPs.
Properties
- Robust (mix-and-read format)
- highly sensitive
- Ideal for a variety of biological samples
- Fast - less than 10 min hands-on time
- Providing results in less than 30 min
EnzoLyte™ 520 assay kits use the FRET substrates that incorporate QXL™ 520 non-fluorescent dye, the best quencher available for 5-FAM. These novel MMP assay kits are widely used for screening MMP activities or MMP inhibitors.
The EnzoLyte Plus™ 520 MMP assay kits are designed for the specific detection of the activity of a particular MMP in a biological mixed sample, which may contain multiple MMPs. A monoclonal anti-human-MMP antibody is used in the kit to pull down both the pro and active forms of an MMP from the mixture, and its proteolytic activity is quantitated using a 5-FAM/QXL™ 520 FRET peptide.
References:
1. Bickett, D. M. et al. Anal. Biochem. 212, 58 (1993). |
2. Knight, C. G. et al. FEBS Lett. 297, 263 (1992). |
3. Netzel-Arnett, S. et al. Anal. Biochem. 195, 86 (1991). |
4. Berman, J. et al. J. Biol. Chem. 267, 1434 (1992). |
5. McGeehan, G. M. et al. J. Biol. Chem. 269, 32814 (1994). |
6. Murphy, G. et al. J. Biol. Chem. 269, 6632 (1994). |
7. Weingarten, H. et al. Biochem. 24, 6730 (1985); |
8. Weingarten, H. et al. Anal. Biochem. 147, 437 (1985). |
9. Bickett, D. M. et al. Ann. N.Y. Acad. Sci. 732, 351 (1994). |
10.Welch, A. R. et al. Arch. Biochem. Biophys. 324, 59 (1995). |
11. Knauper, V. et al. J. Biol. Chem. 271, 1544 (1996). |
12. Nagase, H. et al. J. Biol. Chem. 269, 20952 (1994). |
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Click on the respective order # to download a detailed data sheed. All prices are euro prices excluding shipment and VAT.
Please refer to order code MTJFM for getting the discount. |
| Order # |
Product |
Unit Size |
List Price |
Offer Price |
| MMP Assay Kits |
| 71128AS |
EnzoLyte 490 MMP-1 Assay Kit *Fluorimetric* |
1 kit |
333,00 |
266,40 |
| 71137AS |
EnzoLyte 490 MMP-12 Assay Kit *Fluorimetric* |
1 kit |
333,00 |
266,40 |
| 71135AS |
EnzoLyte 490 MMP-13 Assay Kit *Fluorimetric* |
1 kit |
333,00 |
266,40 |
| 71129AS |
EnzoLyte 490 MMP-2 Assay Kit *Fluorimetric* |
1 kit |
333,00 |
266,40 |
| 71130AS |
EnzoLyte 490 MMP-3 Assay Kit *Fluorimetric* |
1 kit |
333,00 |
266,40 |
| 71132AS |
EnzoLyte 490 MMP-7 Assay Kit *Fluorimetric* |
1 kit |
333,00 |
266,40 |
| 71133AS |
EnzoLyte 490 MMP-8 Assay Kit *Fluorimetric* |
1 kit |
333,00 |
266,40 |
| 71134AS |
EnzoLyte 490 MMP-9 Assay Kit *Fluorimetric* |
1 kit |
333,00 |
266,40 |
| 71158AS |
EnzoLyte 520 Generic MMP Assay Kit *Fluorimetric* |
1 kit |
333,00 |
266,40 |
| 71136AS |
EnzoLyte 520 MMP Profiling Kit *Fluorimetric* |
1 kit |
559,00 |
447,20 |
| 71170AS |
EnzoLyte 520 MMP Substrate Sampler Kit *Fluorimetric* |
1 kit |
559,00 |
447,20 |
| 71150AS |
EnzoLyte 520 MMP-1 Assay Kit *Fluorimetric* |
1 kit |
333,00 |
266,40 |
| 71157AS |
EnzoLyte 520 MMP-12 Assay Kit *Fluorimetric* |
1 kit |
333,00 |
266,40 |
| 71156AS |
EnzoLyte 520 MMP-13 Assay Kit *Fluorimetric* |
1 kit |
333,00 |
266,40 |
| 71151AS |
EnzoLyte 520 MMP-2 Assay Kit *Fluorimetric* |
1 kit |
333,00 |
266,40 |
| 71152AS |
EnzoLyte 520 MMP-3 Assay Kit *Fluorimetric* |
1 kit |
333,00 |
266,40 |
| 71153AS |
EnzoLyte 520 MMP-7 Assay Kit *Fluorimetric* |
1 kit |
333,00 |
266,40 |
| 71154AS |
EnzoLyte 520 MMP-8 Assay Kit *Fluorimetric* |
1 kit |
333,00 |
266,40 |
| 71155AS |
EnzoLyte 520 MMP-9 Assay Kit *Fluorimetric* |
1 kit |
333,00 |
266,40 |
| 72012AS |
EnzoLyte Plus 520 MMP-1 Assay Kit *Fluorimetric and Enhanced Selectivity* |
1 kit |
299,00 |
239,20 |
| 72019AS |
EnzoLyte Plus 520 MMP-13 Assay Kit *Fluorimetric and Enhanced Selectivity* |
1 kit |
299,00 |
239,20 |
| 72017AS |
EnzoLyte Plus 520 MMP-9 Assay Kit *Fluorimetric and Enhanced Selectivity* |
1 kit |
299,00 |
239,20 |
| 72004AS |
Human MMP-1 |
100 uL |
57,00 |
45,60 |
| 72010AS |
Human MMP-12 (100 uL; 10 ug/mL) |
100 uL |
57,00 |
45,60 |
| 72011AS |
Human MMP-13 |
100 uL |
57,00 |
45,60 |
| 72005AS |
Human MMP-2 |
100 uL |
57,00 |
45,60 |
| 72006AS |
Human MMP-3 (100 uL; 10 ug/mL) |
100 uL |
57,00 |
45,60 |
| 72007AS |
Human MMP-7 (100 uL; 10 ug/mL) |
100 uL |
57,00 |
45,60 |
| 72008AS |
Human MMP-8 (100 uL; 10 ug/mL) |
100 uL |
57,00 |
45,60 |
| 72009AS |
Human MMP-9 |
100 uL |
57,00 |
45,60 |
| Order # |
Product |
Unit Size |
List Price |
Offer Price |
| Matrix Metalloproteinases peptides |
| 27074AS |
360 MMP FRET Substrate I |
1 mg |
79,00 |
63,20 |
| 27075AS |
360 MMP FRET Substrate I |
5 mg |
316,00 |
252,80 |
| 27082AS |
360 MMP FRET Substrate II |
1 mg |
68,00 |
54,40 |
| 27083AS |
360 MMP FRET Substrate II |
5 mg |
271,00 |
216,80 |
| 27094AS |
360 MMP FRET Substrate III |
1 mg |
68,00 |
54,40 |
| 27095AS |
360 MMP FRET Substrate III |
5 mg |
271,00 |
216,80 |
| 27098AS |
360 MMP FRET Substrate IV |
1 mg |
68,00 |
54,40 |
| 27099AS |
360 MMP FRET Substrate IV |
5 mg |
271,00 |
216,80 |
| 27102AS |
360 MMP FRET Substrate V |
1 mg |
68,00 |
54,40 |
| 27103AS |
360 MMP FRET Substrate V |
5 mg |
271,00 |
216,80 |
| 27106AS |
360 MMP FRET Substrate VI |
1 mg |
68,00 |
54,40 |
| 27107AS |
360 MMP FRET Substrate VI |
5 mg |
271,00 |
216,80 |
| 27076AS |
390 MMP FRET Substrate I |
1 mg |
79,00 |
63,20 |
| 27077AS |
390 MMP FRET Substrate I |
5 mg |
316,00 |
252,80 |
| 27078AS |
390 MMP FRET Substrate II |
1 mg |
79,00 |
63,20 |
| 27079AS |
390 MMP FRET Substrate II |
5 mg |
316,00 |
252,80 |
| 27090AS |
390 MMP FRET Substrate III |
1 mg |
79,00 |
63,20 |
| 27091AS |
390 MMP FRET Substrate III |
5 mg |
316,00 |
252,80 |
| 27108AS |
390 MMP FRET Substrate IV |
1 mg |
79,00 |
63,20 |
| 27109AS |
390 MMP FRET Substrate IV |
5 mg |
316,00 |
252,80 |
| 27104AS |
490 MMP FRET Substrate III |
1 mg |
203,00 |
162,40 |
| 27105AS |
490 MMP FRET Substrate III |
5 mg |
814,00 |
651,20 |
| 60637AS |
490 MMP FRET Substrate IV |
0.1 mg |
57,00 |
45,60 |
| 60563-1AS |
490 MMP FRET Substrate V |
1 mg |
339,00 |
271,20 |
| 60552-1AS |
490 MMP FRET Substrate VI |
1 mg |
169,00 |
135,20 |
| 60553-1AS |
490 MMP FRET Substrate VII |
1 mg |
169,00 |
135,20 |
| 60568-01AS |
520 MMP FRET Substrate I |
0.1 mg |
107,00 |
85,60 |
| 60569-01AS |
520 MMP FRET Substrate II |
0.1 mg |
107,00 |
85,60 |
| 60570-01AS |
520 MMP FRET Substrate III |
0.1 mg |
107,00 |
85,60 |
| 60571-01AS |
520 MMP FRET Substrate IV |
0.1 mg |
107,00 |
85,60 |
| 60576-01AS |
520 MMP FRET Substrate IX |
0.1 mg |
107,00 |
85,60 |
| 60572-01AS |
520 MMP FRET Substrate V |
0.1 mg |
107,00 |
85,60 |
| 60573-01AS |
520 MMP FRET Substrate VI |
0.1 mg |
107,00 |
85,60 |
| 60574-01AS |
520 MMP FRET Substrate VII |
0.1 mg |
107,00 |
85,60 |
| 60575-01AS |
520 MMP FRET Substrate VIII |
0.1 mg |
107,00 |
85,60 |
| 60577-01AS |
520 MMP FRET Substrate X |
0.1 mg |
107,00 |
85,60 |
| 60578-01AS |
520 MMP FRET Substrate XI |
0.1 mg |
107,00 |
85,60 |
| 60579-01AS |
520 MMP FRET Substrate XII |
0.1 mg |
107,00 |
85,60 |
| 60580-01AS |
520 MMP FRET Substrate XIII |
0.1 mg |
107,00 |
85,60 |
| 60581-01AS |
520 MMP FRET Substrate XIV |
0.1 mg |
107,00 |
85,60 |
| 60582-01AS |
520 MMP FRET Substrate XV |
0.1 mg |
107,00 |
85,60 |
| 60583-01AS |
520 MMP FRET Substrate XVI |
0.1 mg |
107,00 |
85,60 |
| 60554-1AS |
520 MMP FRET Substrate XVII |
1 mg |
249,00 |
199,20 |
| 60585-01AS |
580 MMP FRET Substrate I |
0.1 mg |
107,00 |
85,60 |
| 60555-1AS |
580 MMP FRET Substrate I |
1 mg |
316,00 |
252,80 |
| 60584-01AS |
5-FAM MMP FRET Peptide Fluorescence Standard I |
0.1 mg |
107,00 |
85,60 |
| 27159AS |
Mca MMP FRET Peptide Fluorescence Standard I |
1 mg |
34,00 |
27,20 |
| 27160AS |
Mca MMP FRET Peptide Fluorescence Standard I |
5 mg |
136,00 |
108,80 |
| 27112AS |
Mca MMP FRET Peptide Fluorescence Standard II |
1 mg |
56,00 |
44,80 |
| 27113AS |
Mca MMP FRET Peptide Fluorescence Standard II |
5 mg |
226,00 |
180,80 |
| 27080AS |
MMP Biotinylated Substrate I |
1 mg |
56,00 |
44,80 |
| 27081AS |
MMP Biotinylated Substrate I |
5 mg |
226,00 |
180,80 |
| 27096AS |
MMP Colorimetric Substrate I |
1 mg |
90,00 |
72,00 |
| 27097AS |
MMP Colorimetric Substrate I |
5 mg |
362,00 |
289,60 |
| 27084AS |
MMP HPLC Substrate I |
1 mg |
79,00 |
63,20 |
| 27085AS |
MMP HPLC Substrate I |
5 mg |
316,00 |
252,80 |
| 27086AS |
MMP HPLC Substrate II |
1 mg |
192,00 |
153,60 |
| 27087AS |
MMP HPLC Substrate II |
5 mg |
768,00 |
614,40 |
| 27088AS |
MMP HPLC Substrate III |
1 mg |
192,00 |
153,60 |
| 27089AS |
MMP HPLC Substrate III |
5 mg |
768,00 |
614,40 |
| 27157AS |
MMP Peptide Inhibitor I |
1 mg |
45,00 |
36,00 |
| 27158AS |
MMP Peptide Inhibitor I |
5 mg |
181,00 |
144,80 |
| 27110AS |
NFF-2 |
1 mg |
79,00 |
63,20 |
| 27111AS |
NFF-2 |
5 mg |
316,00 |
252,80 |
| 27114AS |
NFF-3 |
1 mg |
79,00 |
63,20 |
| 27115AS |
NFF-3 |
5 mg |
316,00 |
252,80 |
| 27061AS |
TNO113 |
1 mg |
271,00 |
216,80 |
| 27062AS |
TNO113 |
5 mg |
1085,00 |
868,00 |
| 25350AS |
TNO211 |
1 mg |
203,00 |
162,40 |
| 25350-5AS |
TNO211 |
5 mg |
814,00 |
651,20 |
| 60431-1AS |
WAAG-3R |
1 mg |
186,00 |
148,80 |
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 Download MMP Flyer (70 kb)
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