- Measurement of β-galactosidase activity of yeast clones in yeast two-hybrid screens
- Quantification of the strength of interaction of defined protein pairs in yeast two-hybrid assays
- Measurement of β-galactosidase reporter gene activity in yeast , mammalian or bacterial cells
β-galactosidase assays are crucial to quantitate interactions in yeast two-hybrid screening. In a typical yeast two-hybrid or Y2H Membrane Protein screen, dozens or even hundreds of putative interactors are often identified.
Commonly used assays to detect β-galactosidase activity, such as the filter lift assay or the ONPG assay, are either not quantitative or very cumbersome to use.
Our Y2H kit allows a quick, quantitative measurement of β-galactosidase levels from hundreds of putative prey clones at the same time. The minimal number of handling steps involved ensures that even very large numbers of clones can be processed very fast and easily. The quantitative readout allows you to estimate the interaction strength of every interactor.
The kit combines one-step cell lysis and color development in a format that allows you to perform up to 96 assays in a single experiment, with minimal hands-on time.
The Y2H β-galactosidase kit is also suitable for other applications in yeast, mammalian cells and bacteria that use β-galactosidase as a reporter.