Yeast strains

MoBiTec offers the newest generation of triple yeast reporter stains for highly reliable yeast two-hybrid results (Usable for Y2H Membrane Protein System & Y2H Transactivating Protein System technology)

• Reduce the rate of false positives by enhancing the stringency of your screen
• Our yeast expression strains are especially designed for the expression of high levels of recombinant proteins
• Additional deletion of major proteases in our expression strains guarantees prevention of non-specific degradation

NMY51

Optimized screening strain for conventional yeast two-hybrid and Y2H Membrane screens. Two growth markers and a lacZ reporter ensure the highest possible stringency in a library screen, resulting in fewer false positives. This strain is part of the Y2H Transactivating Protein System and Y2H Membrane Protein System starter kits.
MATa his3delta200 trp1-901 leu2-3,112 ade2 LYS2::(lexAop)4-HIS3
ura3::(lexAop)8-lacZ (lexAop)8-ADE2 GAL4)

NMY61

Mating partner for reporter strain NMY51.
MATalpha his3delta200 trp1-901 leu2-3,112 ade2 LYS2::(lexAop)4-HIS3 ura3::(lexAop)8-lacZ (lexAop)8-ADE2 GAL4)

DSY-5

General purpose strain for expression of recombinant proteins in yeast. Deletion of the two major vacuolar proteases Pep4 and Prb1 ensures good yield and little non-specific degradation when purifying proteins from yeast. Can be used in combination with our general purpose yeast expression vectors.
MATalpha leu2 trp1 ura3-52 his3::GAL1-GAL4 pep4 prb1-1122

DSY-6

DSY-6 has been designed for expression of recombinant proteins in yeast. Good growth characteristics ensure high cell densities both in synthetic defined (SD) and rich (YPD) medium. Deletion of prb1 encoding the vacuolar proteinase B (yscB), pep4 encoding the vacuolar aspartyl protease (proteinase A) and prc1 encoding the vacuolar carboxypeptidase Y (proteinase C) ensures minimal protein degradation during cell lysis and protein extraction.
MATa leu2 trp1delta63 ura3-52 prb1-22 pep4-3 prc1-407