Products | Proteomics

 

Bacterial Protein Extraction

 
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BactoLyse Bacterial Protein Extraction Reagent

     
   
Features
  • Highly efficient yet gentle method for extracting proteins from bacteria cells
  • Proprietary nonionic detergent in 50 mM NaHPO4 (pH 7.4)
  • No need for time-consuming and cumbersome mechanical disruption methods
  • Several fold increase in the yield of soluble proteins when compared with other commercial lysis reagents
  • Proteins are suitable for affinity purification and analysis
  • Reagent may also be used for preparation of high purity inclusion bodies from total cell lysates

For some proteins, better extraction and purification results are achieved with BactoLyse bacterial protein extraction reagent in 20 mM Tris-HCl (pH 7.5).


     
     

Order Information

Please click on the respective order # for data sheets.
BactoLyse Bacterial Protein Extraction Reagent
order# description amount
PR-PEB10100-01 BactoLyse bacterial protein extraction reagent 125 ml
PR-PEB10100-02 BactoLyse bacterial protein extraction reagent 500 ml
shipped at RT; store at RT


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BactoLyse in Tris Buffer

     
     
Features
  • Highly efficient yet gentle method for extracting proteins from bacteria cells
  • Proprietary nonionic detergent in in 20 mM Tris-HCl (pH 7.5)
  • No need for time-consuming and cumbersome mechanical disruption methods
  • Several fold increase in the yield of soluble proteins when compared with other commercial lysis reagents
  • Proteins are suitable for affinity purification and analysis
  • Reagent may also be used for preparation of high purity inclusion bodies from total cell lysates

For some proteins, better extraction and purification results are achieved with BactoLyse reagent in Phosphate Buffer.


     
     

Order Information

Please click on the respective order # for data sheets.
BactoLyse in Tris Buffer
order# description amount
PR-PEB10110-01 BactoLyse in Tris buffer 125 ml
PR-PEB10110-02 BactoLyse in Tris buffer 500 ml
shipped at RT; store at RT


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QuickCells
order# description amount
CB-CEC50200-01 QuickCells BL21(DE3) Chemically Competent
E. coli
20 x 50 μl
CB-CEC50250-01 QuickCells BL21(DE3)pLysS Chemically Competent E. coli 20 x 50 μl


Staining and Detection
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DB001 Deep Blue 0.5 l
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ENL01 Enlight 0.5 l
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