products | DNA vector systems | ssDNA - Production and Expression in pMEX

DNA Vector Systems

ssDNA - Production and Expression in pMEX

Features:

• cloning
• strong gene expression
• sequencing
• unidirectional deletion
  

Product Description:

The pMEX DNA cloning vectors combine the cloning and expression advantages of DNA plasmid (orcosmid) systems with the sequencing and mutagenesis advantages of single-strand phage systems.
The pMEX vectors contain the ColE1 origin of replication and the phage f1 intergenic region. Thus, genes and gene banks can be cloned, sequenced, mutagenized, and expressed in the same vector system.

To optimize gene expression, the distance between the promoter and the ribosomal binding site (Shine-Dalgarno sequence) can be easily shortened to the optimal length of 5 to 20 base pairs using the easy access to site directed mutagenesis. With the strong promoter, this results in strong expression of the cloned gene product.
The MCS is constructed such that the cloned gene can be sequenced easily by nested deletions from both sides (5´ and 3´) whilst the framing genetic elements (operator, promoter, start and stop codons, terminators) and both sequencing primer sequences remain unchanged. This also enables protein engineering by truncation from both sides with stable gene expression.

The pMEX DNA vector system is easy to handle. E.coli lacI^q strains can be used, which have advantages for the expression of toxic proteins. The strong promoter does not require heat shock activation; thus, no SOS response will interfere with the protein expression.

Multiple Cloning Sites:

pMEX5 and pMEX6:

pMEX7 and pMEX8:

pMEX 3´ Primer: 3´-CCGCCTACTCTCTTCT-5´ (for pMEX6 and pMEX8)

See also chapter 4 of our online catalog.

Downloads: Handbook
pMEX-sequences