Frequently used standard vectors at high quality

products | DNA vector systems | Standard Cloning Vectors

DNA Vector Systems

Standard Cloning Vectors

Features:

highest quality DNA prepared by
- ion-exchange chromatography,
- cesium chloride density centrifugation and
- gel filtration
preparations with over 80% supercoiling
of special interest: pUC118 and pUC119
ready-to-use for:
- transformations
- enzymatic reactions
competitive price!

pBR322:

pBR322 is an ampicillin and tetracycline resistant, general purpose cloning vector. Several inactivating cloning sites are present in the antibiotics resistance genes, which, if a reading frame shift occurs, will give rise to Amp^R /Tc^S or, alternatively, Amp^S /Tc^R transformants. Cloning sites are indicated on the data sheet.

pBR325:

pBR325 is an ampicillin, tetracycline and chloramphenicol resistant, general purpose cloning vector. It is derived from pBR322 by insertion (into its EcoR I site) of the chloramphenicol acetyltransferase gene.

pBR328:

pBR328 is an ampicillin, tetracycline and chloramphenicol resistant, general purpose cloning vector. This vector, derived from pBR325, has the bom site (basis of mobility) deleted and therefore is non-mobilizable; this makes pBR328 suitable where more stringent biological containment is required. This deletion also creates extra unique cloning sites in the chloramphenicol acetyltransferase gene: Pvu II, BspM II and Bal I.

Vector	Resistance	Origin	Speciality
pBR322	Tc, Amp	ColE1	reading frame shift gives Amp^R , Tc^S transformants
pBR325	Tc, Amp, Cm	ColE1	Cm resistant pBR322
pBR328	Tc, Amp, Cm	ColE1	non-mobilizable pBR325
pACYC184	Tc,Cm	p15A1	double-transformants with ColE1 vectors
pAT153	Tc, Amp	ColE1	non-mobilizable; higher copy than pBR322
pUC18	Amp	ColE1	MCS within lacZ: blue/white selection
pUC118	Amp	ColE1	M13 origin for ssDNA production
pUC19	Amp	ColE1	MCS within lacZ:blue/white selection
pUC119	Amp	ColE1	M13 origin for ssDNA production

pUC18/19 and pUC118/119:

The plasmids have a multiple cloning site within the lacZ alpha-fragment. Inserts cloned into this site disrupt beta -galactosidase activity and give rise to white colonies on X-Gal/IPTG plates. The plasmids encode resistance to ampicillin. Foreign DNA inserted in-frame with the lac Z gene will be expressed as a fusion protein (containing a portion of the beta-galactosidase) under control of the lac promoter. The promoter is inducible with IPTG and followed by an initiation codon as well as a ribosome binding site. pUC18 and pUC19 differ in their multiple cloning site orientation. pUC118 and pUC119 contain an additional M13 phage origin for single strand production.

pACYC184:

pACYC184 encodes tetracycline and chloramphenicol resistance. Unlike most cloning vectors, which have ColE1 origins of replication, pACYC184 has an origin derived from p15A1. This allows pACYC184 to be maintained in a pBR322 or pUC18 transformant, for example. Such a double transformant is necessary where two recombinant proteins need to be expressed simultaneously.

pAT153:

pAT153 is a derivative of pBR322 where the bom (basis of mobility) site has been deleted. Thus pAT153 is non-mobilizable and is more readily contained than pBR322. Also in this 703 bp deletion was the region involved in copy number control; loss of this region gives pAT153 a 1.5 to 3-fold higher copy number. pAT153 encodes ampicillin and tetracycline resistance.

For a detailed data sheet as a pdf-file, please click on the product No.

ORDER INFORMATION, SHIPPING STORAGE:
order#	description	amount*
V30302	pBR322 vector DNA	25 µg
V30402	pBR325 vector DNA	25 µg
V32802	pBR328 vector DNA	25 µg
V32402	pACYC184 vector DNA	25 µg
V32602	pAT153 vector DNA	25 µg
V33002	pUC18 vector DNA	25 µg
V33302	pUC118 vector DNA	25 µg
V33202	pUC19 vector DNA	25 µg
V33402	pUC119 vector DNA	25 µg
shipped on blue ice; store at -20°C; *DNA is in solution

See also chapter 4 of our online catalog.

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