Tet-Repressor Antibodies

Product Description:

The tetracycline (tet) regulatory system is widely used for selective target gene regulation in eukaryotic cells. MoBiTec now offers a unique set of polyclonal and monoclonal antibodies targeting the Tet-Repressor protein (TetR) (TetR-tetO Accession no. P04483) for study of this popular system. These antibodies possess excellent binding properties and have been successfully tested for use in ELISA, Western blot and immunofluorescence assays. Two options for the monoclonal antibodies are offered. First an optimized mix consisting of the two different epitope-specific monoclonal antibodies (TET02), and second a single monoclonal antibody, which can be used for immunofluorescence microscopy (TET03). The rabbit polyclonal antibody (TET01) can be used in all three above-mentioned applications. These antibodies provide an excellent new tool for elucidating the tet regulatory system.

Features:

• new tool for studies of tet regulatory system in eukaryotic cells
• suited for ELISA, Western blots and immunofluorescence
• excellent binding properties
• monoclonal IgG1;
• polyclonal rabbit IgG
• Immunogen: TetR-tetO
  Accession no. P04483 Pubmed
• Tet-Repressor positive control protein also available
  
  
  

Perfectly suited for detection of:

• Tet-Repressor (TetR)
• Tet-Repressor fusion protein (TetR-Fusion)
• tetracycline responsive transactivator (tTA)
• reverse tetracycline responsive transactivator (rtTA) including derivates like rtTA-S or rtTA-M

Summery of the three Tet-Repressor Antibodies

	TET01	TET02	TET03
Type	Rabbit polyclonal IgG	monoclonal IgG1; mix	monoclonal IgG1;
Immunogen	TetR-tetO	TetR-tetO	TetR-tetO
Purification	Affinity purified via Protein G columns	Affinity purified via Protein A or G columns	Affinity purified via Protein A or G columns
Epitope	-	TetR: - Amino acid # 84 - 98 - Amino acid # 26 - 53	TetR: - Amino acid # 37 - 44
Reconstitution in	200 µl dest. H2O	100 µl dest. H2O	100 µl dest. H2O
Working dilution for immunofluorescence	n.d.	n.d.	1:100 - 1:500
Working Dilution for Western blots and ELISA	1:1000	1:500 - 1:2000	1:1000
Detection limit ELISA	0.2 ng	20 - 50 pg	n.d.
Detection limit Western Blot	0.8 ng	0.8 - 1.0 ng	5 ng

Examples of different application

For a larger image with detailed description please click on the images

Literature

Tet regulatory system:

1. Pook E, Grimm S, Bonin A, Winkler T, Hillen W.(1998): Affinities of mAbs to Tet repressor complexed with operator or tetracycline suggest conformational changes associated with induction.
   Eur J Biochem. 1998 Dec 15;258(3):915-22. Pubmed
   
   
2. Hillen W, Berens C.(1994): Mechanisms underlying expression of Tn10 encoded tetracycline resistance.
   Annu Rev Microbiol. 1994;48:345-69. Pubmed
   
   
3. Gatz C.(1995): Novel inducible/repressible gene expression systems.
   Methods Cell Biol. 1995;50:411-24. Pubmed
   
   
4. Mayford M, Bach ME, Huang YY, Wang L, Hawkins RD, Kandel ER.(1996): Control of memory formation through regulated expression of a CaMKII transgene.
   Science. 1996 Dec 6;274(5293):1678-83. Pubmed
   
   
5. Hwang JJ, Scuric Z, Anderson WF. (1996): Novel retroviral vector transferring a suicide gene and a selectable marker gene with enhanced gene expression by using a tetracycline-responsive expression system.
   J Virol. 1996 Nov;70(11):8138-41. Pubmed Free
   
   
6. Gossen M, Freundlieb S, Bender G, Muller G, Hillen W, Bujard H. (1996): Transcriptional activation by tetracyclines in mammalian cells. Pubmed
   Science. 1995 Jun 23;268(5218):1766-9.
   
   
7. Hinrichs W, Kisker C, Duvel M, Muller A, Tovar K, Hillen W, Saenger W (1994): Structure of the Tet repressor-tetracycline complex and regulation of antibiotic resistance.
   Science. 1994 Apr 15;264(5157):418-20. Pubmed
   
   

References with Tet-Repressor (TetR) Antibodies

8. Marti F, Xu CW, Selvakumar A, Brent R, Dupont B, King PD. (1998): LCK-phosphorylated human killer cell-inhibitory receptors recruit and activate phosphatidylinositol 3-kinase.
   Proc Natl Acad Sci U S A. 1998 Sep 29;95(20):11810-5. Pubmed Free
   
   
9. Freundlieb S, Schirra-Muller C, Bujard H.(1999): A tetracycline controlled activation/repression system with increased potential for gene transfer into mammalian cells.
   J Gene Med. 1999 Jan-Feb;1(1):4-12. Pubmed
   
   
10. Urlinger S, Baron U, Thellmann M, Hasan MT, Bujard H, Hillen W. (2000): Exploring the sequence space for tetracycline-dependent transcriptional activators: novel mutations yield expanded range and sensitivity.
    Proc Natl Acad Sci U S A. 2000 Jul 5;97(14):7963-8. Pubmed Free
    
    
11. Meissner M, Brecht S, Bujard H, Soldati D. (2001): Modulation of myosin A expression by a newly established tetracycline repressor-based inducible system in Toxoplasma gondii.
    Nucleic Acids Res. 2001 Nov 15;29(22):E115. Pubmed Free
    
    
12. Lorenz P, Koczan D, Thiesen HJ (2001): Transcriptional repression mediated by the KRAB domain of the human C2H2 zinc finger protein Kox1/ZNF10 does not require histone deacetylation.
    Biol Chem. 2001 Apr;382(4):637-44. Pubmed
    
    
13. Fiedler M, Skerra A (2001): proBA complementation of an auxotrophic E. coli strain improves plasmid stability and expression yield during fermenter production of a recombinant antibody fragment.
    Gene. 2001 Aug 22;274(1-2):111-8. Pubmed
    
    
14. Stebbins MJ, Urlinger S, Byrne G, Bello B, Hillen W, Yin JC (2001): Tetracycline-inducible systems for Drosophila.
    Proc Natl Acad Sci U S A. 2001 Sep 11;98(19):10775-80. Epub 2001 Aug 21. Pubmed Free
    
    
15. David KM, Perrot-Rechenmann C (2001): Characterization of a tobacco Bright Yellow 2 cell line expressing the tetracycline repressor at a high level for strict regulation of transgene expression.
    Plant Physiol. 2001 Apr;125(4):1548-53. Pubmed Free
    
    
16. Reeves PJ, Callewaert N, Contreras R, Khorana HG (2002): Structure and function in rhodopsin: high-level expression of rhodopsin with restricted and homogeneous N-glycosylation by a tetracycline-inducible N-acetylglucosaminyltransferase I-negative HEK293S stable mammalian cell line.
    Proc Natl Acad Sci U S A. 2002 Oct 15;99(21):13419-24. Epub 2002 Oct 7. Pubmed Free
    
    
17. Kamper MR, Gohla G, Schluter G (2002): A novel positive tetracycline-dependent transactivator (rtTA) variant with reduced background activity and enhanced activation potential.
    FEBS Lett. 2002 Apr 24;517(1-3):115-20. Pubmed
    
    
18. Krueger C, Berens C, Schmidt A, Schnappinger D, Hillen W (2003): Single-chain Tet transregulators.
    Nucleic Acids Res. 2003 Jun 15;31(12):3050-6. Pubmed Free
    
    
19. Czauderna F, Santel A, Hinz M, Fechtner M, Durieux B, Fisch G, Leenders F, Arnold W, Giese K, Klippel A, Kaufmann J (2003): Inducible shRNA expression for application in a prostate cancer mouse model.
    Nucleic Acids Res. 2003 Nov 1;31(21):e127. Pubmed Free
    
    
20. Leenders F, Mopert K, Schmiedeknecht A, Santel A, Czauderna F, Aleku M, Penschuck S, Dames S, Sternberger M, Rohl T, Wellmann A, Arnold W, Giese K, Kaufmann J, Klippel A (2004): PKN3 is required for malignant prostate cell growth downstream of activated PI 3-kinase.
    EMBO J. 2004 Aug 18;23(16):3303-13. Epub 2004 Jul 29. Pubmed Free
    
    
21. Das AT, Zhou X, Vink M, Klaver B, Verhoef K, Marzio G, Berkhout B (2004): Viral evolution as a tool to improve the tetracycline-regulated gene expression system.
    J Biol Chem. 2004 Apr 30;279(18):18776-82. Epub 2004 Feb 2. Pubmed Free
    
    
22. Rupp B, Ruzsics Z, Sacher T, Koszinowski UH (2005): Conditional cytomegalovirus replication in vitro and in vivo.
    J Virol. 2005 Jan;79(1):486-94. Pubmed Free
    
    
23. Markusic D, Oude-Elferink R, Das AT, Berkhout B, Seppen J (2005): Comparison of single regulated lentiviral vectors with rtTA expression driven by an autoregulatory loop or a constitutive promoter.
    Nucleic Acids Res. 2005 Apr 4;33(6):e63. Pubmed Free
    
    
24. Osada M, Park HL, Nagakawa Y, Yamashita K, Fomenkov A, Kim MS, Wu G, Nomoto S, Trink B, Sidransky D (2005): Differential recognition of response elements determines target gene specificity for p53 and p63.
    Mol Cell Biol. 2005 Jul;25(14):6077-89. Pubmed Free
    
    
25. Ito T, Hashimoto Y, Tanaka E, Kan T, Tsunoda S, Sato F, Higashiyama M, Okumura T, Shimada Y (2006): An inducible short-hairpin RNA vector against osteopontin reduces metastatic potential of human esophageal squamous cell carcinoma in vitro and in vivo.
    Clin Cancer Res. 2006 Feb 15;12(4):1308-16. Pubmed
    
    
26. Cai D, Byth KF, Shapiro GI (2006): AZ703, an imidazo[1,2-a]pyridine inhibitor of cyclin-dependent kinases 1 and 2, induces E2F-1-dependent apoptosis enhanced by depletion of cyclin-dependent kinase 9.
    Cancer Res. 2006 Jan 1;66(1):435-44. Pubmed
    
    
27. Ito T, Hashimoto Y, Tanaka E, Kan T, Tsunoda S, Sato F, Higashiyama M, Okumura T,Shimada Y (2006): An Inducible Short-Hairpin RNA Vector against Osteopontin Reduces Metastatic Potential of Human Esophageal Squamous Cell Carcinoma In vitro and In vivo
    Clin. Cancer Res., Feb 2006; 12: 1308 - 1316. Pubmed
    
    
28. Lukes J, Paris Z, Regmi S, Breitling R, Mureev S, Kushnir S, Pyatkov K, Jirku M, Alexandrov KA (2006): Translational initiation in Leishmania tarentolae and Phytomonas serpens (Kinetoplastida) is strongly influenced by pre-ATG triplet and its 5' sequence context.
    Mol Biochem Parasitol. 2006 Aug;148(2):125-32. Epub 2006 Apr 18. Pubmed
    
    
29. Cai D, Vaughan M. Latham, Jr., Xinxin Zhang, and Geoffrey I. Shapiro (2006): Combined Depletion of Cell Cycle and Transcriptional Cyclin-Dependent Kinase Activities Induces Apoptosis in Cancer Cells
    Cancer Res., Sep 2006; 66: 9270 - 9280. Pubmed
    
    
30. Bulliard Y, Wiznerowicz M, Barde I, Trono D (2006): KRAB Can Repress Lentivirus Proviral Transcription Independently of Integration Site
    J. Biol. Chem., Nov 2006; 281: 35742 - 35746. Pubmed
    
    
31. Palona I, Namba H, Mitsutake N, Starenki D, Podtcheko A, Sedliarou I, Ohtsuru A, Saenko V, Nagayama Y, Umezawa K, Yamashita S (2006): BRAFV600E promotes invasiveness of thyroid cancer cells through nuclear factor kappaB activation.
    Endocrinology, Dec 2006; 147: 5699 - 5707. Pubmed
    
    
32. Seibler J, Kleinridders A, Küter-Luks B, Niehaves S, Brüning JC, Schwenk F (2007): Reversible gene knockdown in mice using a tight, inducible shRNA expression system
    Nucleic Acids Res., Apr 2007; 35: e54. Pubmed
    
    
33. Carrillo J, García-Aragoncillo E, Azorín D, Agra N, Sastre A, González-Mediero I, García-Miguel P, Pestaña A, Gallego S, Segura D, Alonso J (2007): Cholecystokinin Down-Regulation by RNA Interference Impairs Ewing Tumor Growth
    Clin. Cancer Res., Apr 2007; 13: 2429 - 2440. Pubmed
    
    
34. Wegmüller D, Raineri I, Gross B, Oakeley EJ, Moroni C (2007): A Cassette System to Study Embryonic Stem Cell Differentiation by Inducible RNA Interference
    Stem Cells, May 2007; 25: 1178 - 1185. Pubmed
    
    
35. Weber A, Paschen SA, Heger K, Wilfling F, Frankenberg T, Bauerschmitt H, Seiffert BM, Kirschnek S, Wagner H, Häcker G (2007): BimS-induced apoptosis requires mitochondrial localization but not interaction with anti-apoptotic Bcl-2 proteins.
    J. Cell Biol., May 2007; 177: 625 - 636. Pubmed
    
    

 
See also chapter 5 of our online catalog.