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Cell Transfection Reagents

  
  MoBiTec - Innovative Tools for Molecular Biology  
button GeneTrans Transfection Reagent
button LowtoxicTrans Biodegradable Transfection Reagent
button MultiTrans Broad-Range Transfection Reagent
button NeuroTrans Transfection Reagent for Neuronal Cells
button GenePusher Reagent
button ProTrans Protein Delivery Reagent
button TransIT® Transfection Reagents
button TransIT-PRO™ Transfection Kit
button TransIT®-LT1 Transfection Reagent
button TransIT®-2020 Transfection Reagent
           
           
 

GeneTrans Gene Delivery Reagents

      
 

Features and Advantages:

  • High transfection efficiencies in multiple cell lines
  • Good results with many hard-to-transfect cell lines
  • Very cost-effective and easy-to-use
  • Best efficiency with Jurkat cells
  • Ideal for oligonucleotide transfection

General Product Description

GeneTrans Transfection Reagents have been developed for highly efficient transfection of a broad variety of cell types. They enable good transfection efficiencies also with many known hard-to-transfect cell lines (for maximal transfection efficiencies with hard-to-transfect cells, we recommend GeneTrans II).
GeneTrans Transfection Reagents utilize the unique Direct Hydrophilic Conjugation (DHC) technology developed by the inventor of lipofection. In contrast to other commercially available transfection reagents, GeneTrans contains an ideally adjusted mixture of differentially modified cationic lipids. It optimizes the assembly of lipid/DNA complexes and significantly reduces cytotoxicity. This ensures highly efficient and reproducible results with both stable and transient transfections. Moreover, the strongly reduced cell damage and the elimination of media changes make GeneTrans Transfection Reagents ideal for all gene expression studies where both optimal gene delivery and the post-transfection state of the cells are important. In addition, they have proven to be perfect for oligonucleotide transfection and for transfecting special cell lines such as Jurkat cells.

 

   Gene Trans-mediated DNA delivery into cells
GeneTrans-mediated DNA delivery into cells.		  
 

GeneTrans I Transfection Reagent

GeneTrans I Transfection Reagent has been designed to accomplish a high and consistent delivery of DNA into a broad range of cell types. It is very easy to use and performs well in wide-ranging conditions. In addition, GeneTrans I Transfection Reagent does not require enhancers or special handling of cells as it is known for other commonly used transfection reagents. It saves time, cost and reagents, and is suitable for both in vitro and in vivo transfection studies. GeneTrans I Transfection Reagent is a unique formulation of the neutral lipid dioleoyl phosphatidylethanolamine (DOPE) and a proprietary cationic lipid that is derived from the innovative Direct Hydrophilic Conjugation (DHC) technology. A small volume (5 µl) of GeneTrans I reagent can be used for efficiently transfecting 1 µg of DNA. GeneTrans I reagent is provided in a dry form which is stable at 4°C for at least 1 year; the hydrated reagent is stable for a minimum of 6 months at 4°C.

  
High-efficient uptake of fluorescein-labeled phosphorothioate oligonucleotides using GeneTrans Transfection Reagent. 3 hours
 
High-efficient uptake of fluorescein-labeled phosphorothioate oligonucleotides using GeneTrans Transfection Reagent. 24 hours
High-efficient uptake of fluorescein-labeled phosphorothioate oligonucleotides using GeneTrans Transfection Reagent. 3 hours (top) and 24 hours (bottom) post-transfection.
 
 


GeneTrans II Transfection Reagent

Features and Advantages

  • Optimized transfection efficiency
  • Greatly improved results with hard-to-transfect cells
  • Ideally suited for oligonucleotide transfection
  • No media changes - transfections can be easily carried out with or without serum
  • Robust reproducible results with cell lines used for protein expression
  • Adaptable to both suspension and adherent cells
  • Minimized cytotoxicity
  • Suitable for both in vitro and in vivo transfection
  • Excellent and reproducible performance with many different cell lines

Product Description

The GeneTrans II Transfection Reagent is the newest advance in gene delivery technology and based on a novel clearly improved formulation that allows a further enhancement of DNA stability and reduction of interference from serum components during the transfection process. Its ability first to stabilize and protect DNA more effectively and second, to facilitate its nuclear uptake into cells renders GeneTrans II a highly effective delivery tool. Additionally, lipoplexes produced with the GeneTrans II formulation have lower interaction with serum components and are thereby in particular very suitable and effective for use in serum containing medium. It includes optimized components and buffers allowing superior transfection efficiencies also with notoriously hard-to-transfect cell lines such as HeLa, K-562, and PC-12. GeneTrans II is ideal for high-throughput screening and protein expression applications and offers clear advantages compared to other leading transfection reagents. GeneTrans II is provided with two diluents (DNA Diluent A and DNA Diluent B) to allow you more flexibility in optimizing nucleic acid transfections for your cell lines of choice. If you are working with easy-to-transfect cell lines in serum-free media, we recommend the original GeneTrans I transfection reagent as an ideal cost-effective alternative. It enables transfection results that are by all means comparable to any competitive reagent on the market.

GeneTrans II comes complete with everything you need for optimizing transfection conditions for your specific cell line(s), including the convenient GeneTrans II dried lipid films, hydration buffer (0.75 ml or 1.5 ml), and two types of DNA diluents (A and B: 4 ml or 8 ml).
For your convenience, a protocol with tested optimized transfection conditions for the most popularly used cell lines is included. By following the simple steps in this comprehensive manual, you can expect optimal transfection results.

  
Comparison of GeneTrans II Transfection reagent with leading reagent L2K
Comparison of GeneTrans II Transfection reagent with leading reagent L2K.
 
                     
  Please click on the respective order # for further details.
ORDER INFORMATION, SHIPPING & STORAGE:
order# description amount
0101B GeneTrans Transfection Reagent (75 rxns) 0.75 ml
0102B GeneTrans Transfection Reagent (150 rxns) 1.5 ml
0103B GeneTrans Transfection Reagent (750 rxns) 5 x 1.5 ml
0104B GeneTrans I QuickEase Kit (96 rxns) 1
0201B GeneTrans II Transfection Reagent (75 rxns) 0.75 ml
0202B GeneTrans II Transfection Reagent (150 rxns) 1.5 ml
0203B GeneTrans II Transfection Reagent (750 rxns) 5 x 1.5 ml
0204B GeneTrans II QuickEase Kit (96 rxns) 1
shipped at RT; store at 4°C


     
               
 
     
 

LowtoxicTrans Biodegradable Transfection Reagent

  
 

Features and Advantages:

  • Biodegradable in vivo
  • Dramatically reduced cytotoxicity
  • Maximized delivery of macromolecules into cells
  • Easy protocol


General Product Description

LowtoxicTrans is a convenient biodegradable cationic polymer that allows for simple same day, high efficiency, and low cost gene delivery in mammalian cell lines. During transfection, the polymer/DNA complexes (polyplexes) are endocytosed into the cells, where the polymer is biodegraded into small non-toxic molecules. The ability of LowtoxicTrans to biodegrade in vivo dramatically reduces its cytotoxicity while maximizing the delivery of macromolecules into cells. The protocol is easy! Simply plate your cell line in complete medium, and immediately add the DNA/LowtoxicTrans polyplexes without the need for overnight incubation. Assay for transgene expression 24 - 48 hours later. LowtoxicTrans is compatible with serum-containing medium, it is simple to use, and it provides high transfection efficiency at a low cost per reaction relative to other commercial transfection reagents.


          
 

Contents

LowtoxicTrans biodegradable transfection reagent (25 reactions)
Kit content 50 μl LowtoxicTrans biodegradable transfection reagent
5 ml Lift-Off detaching Cell Detachment Solution
10 μl, 2 μg Achilles GFP Control Plasmid
LowtoxicTrans biodegradable transfection reagent (375 reactions)
Kit content 0.75 ml LowtoxicTrans biodegradable transfection reagentl
5 ml Lift-Off detaching Cell Detachment Solution
10 μl, 2 μg Achilles GFP Control Plasmid
LowtoxicTrans biodegradable transfection reagent (750 reactions)
Kit content 1.5 ml ml LowtoxicTrans biodegradable transfection reagentl
5 ml Lift-Off detaching Cell Detachment Solution
10 μl, 2 μg Achilles GFP Control Plasmid
LowtoxicTrans biodegradable transfection reagent 3,750 reactions)
Kit content 5 x 1.5 ml ml LowtoxicTrans biodegradable transfection reagentl
5 ml Lift-Off detaching Cell Detachment Solution
10 μl, 2 μg Achilles GFP Control Plasmid
LowtoxicTrans biodegradable transfection reagent (7,500 reactions)
Kit content 10 x 1.5 ml ml LowtoxicTrans biodegradable transfection reagentl
5 ml Lift-Off detaching Cell Detachment Solution
10 μl, 2 μg Achilles GFP Control Plasmid



Order Information, Shipping & Storage

Please click on the respective order # for further details.
Order Information
Order # Description Amount
CB-TDB10300-01 LowtoxicTrans biodegradable transfection reagent
(25 reactions) 		50 µl
CB-TDB10300-02 LowtoxicTrans biodegradable transfection reagent
(375 reactions)		 0.75 ml
CB-TDB10300-03 LowtoxicTrans biodegradable transfection reagent
(750 reactions)		 1.5 ml
CB-TDB10300-04 LowtoxicTrans biodegradable transfection reagent
(3,750 reactions)		 5 x 1.5 ml
CB-TDB10300-05 LowtoxicTrans biodegradable transfection reagent
(7,500 reactions)		 10 x 1.5 ml
shipped on dry ice; store kit at -20 °C
LowtoxicTrans can be stored at 4 °C


Related Products

Order # Description Amount
0201B GeneTrans II Transfection Reagent 75 rxns
0202B GeneTrans II Transfection Reagent 150 rxns
0203B GeneTrans II Transfection Reagent 750 rxns
CB-TDR10100-02 siRTrans siRNA transfection reagent 200 reactions
CB-TDR10100-03 siRTrans siRNA transfection reagent 5 x 200 reactions
0401B ProTrans QuickEase Kit 24 rxns
0402B ProTrans QuickEase Kit 96 rxns
CB-TDN10500-01 NeuroTrans transfection reagent for neuronal cells 0.75 ml
CB-TDN10500-02 NeuroTrans transfection reagent for neuronal cells 5 x 0.75 ml
CB-AOD10100-01 MoBiTec Mycoplasma PCR Detection Kit 50 reactions
CB-AOD10100-02 MoBiTec Mycoplasma PCR Detection Kit 100 reactions


     
               
 
     
 

MultiTrans Broad-Range Transfection Reagent

      
 

Features and Advantages:

  • Superior transfection efficiency
  • Cost effectiveness
  • Compatibility with serum
  • Extended shelf life

 

General Product Description

The MultiTrans broad-range transfection reagent is a unique formulation of the neutral lipid, dioleoyl phosphatidylethanolamine (DOPE), and a proprietary cationic lipid derived from MoBiTec unique Direct Hydrophilic Conjugation (DHC) technology. The MultiTrans broad-range transfection reagent has been specifically optimized for transfection and gene expression in CHO, COS, 293, NIH-3T3, HeLa, and Jurkat cells. Each kit includes a special DNA Diluent that increases transfection efficiency in the presence of serum.
The MultiTrans broad-range transfection reagent is suitable for both in vitro and in vivo transfection studies.

            
 

Components

Components
1 vial MultiTrans Broad-Range Transfection Reagent
0.8 ml Hydration Buffer
5.5 ml DNA Diluent



Order Information, Shipping & Storage

Please click on the respective order # for further details.
Order Information
Order # Description Amount
CB-TDB10400-01 MultiTrans broad-range transfection reagent 0.75 ml
CB-TDB10400-02 MultiTrans broad-range transfection reagent 1.5 ml
shipped at RT; store at 4 °C; stable for 1 year


Related Products

Order # Description Amount
0201B GeneTrans II Transfection Reagent 75 rxns
0202B GeneTrans II Transfection Reagent 150 rxns
0203B GeneTrans II Transfection Reagent 750 rxns
CB-TDT10666-01 GenePusher Reagent 3 x 1.5 ml
CB-TDR10100-02 siRTrans siRNA transfection reagent 200 reactions
CB-TDR10100-03 siRTrans siRNA transfection reagent 5 x 200 reactions
VS-EXC00100-01 phCMV1 CMV-based constitutive expression vector 25 µg
VS-EXC00200-01 phCMV2 CMV-based constitutive expression vector 25 µg
VS-EXC00300-01 phCMV3 CMV-based constitutive expression vector 25 µg
CB-TDN10500-01 NeuroTrans transfection reagent for neuronal cells 5 x 0.75 ml
CB-TDN10500-02 NeuroTrans transfection reagent for neuronal cells 0.75 ml


     
               
 
     
 

NeuroTrans Transfection Reagent for Neuronal Cells

      
 

Features and Advantages

  • Optimal transfection of neuronal cells
  • Biodegradable in vivo
  • Dramatically reduced cytotoxicity


Product Description

NeuroTrans is a novel biodegradable cationic polymer created specifically for optimal transfection of neuronal cells. During transfection, the polymer/DNA complexes (polyplexes) are endocytosed into the cells, where the polymer is biodegraded into small non-toxic molecules. The ability of NeuroTrans to biodegrade in vivo dramatically reduces its cytotoxicity and therefore maximizes the delivery of macromolecules into cells. NeuroTrans is compatible with serum containing media, is easy to use, and provides the highest possible transfection efficiencies for your primary neurons.

            
 

Order Information, Shipping & Storage

Please click on the respective order # for further details.
Order Information
Order # Description Amount
CB-TDN10500-01 NeuroTrans transfection reagent for neuronal cells
(75-300 react.)		 0.75 ml
CB-TDN10500-02 NeuroTrans transfection reagent for neuronal cells
(375-1,500 react.)		 5 x 0.75 ml
shipped at RT; store at 4 °C


     
               
 
     
 

GenePusher Reagent

      
 

Features

  • Economical and easy to use
  • Highest gene expression in diverse cell types
  • Excellent enhancement with GeneTrans Transfection Reagent and GeneTrans II Transfection Reagent
  • Very good improvement with other transfection reagents
  • Effective for both transient and stable transfection
  • Extended shelf life

 

Description

The Gene Pusher Reagents are a set of chemical cocktails to increase the gene expression level obtained with synthetic non-viral DNA delivery systems such as GeneTrans Transfection Reagent and GeneTrans II Transfection Reagent. They also can be used with all other commercially available transfection reagents. Three unique formulations have been developed for different cell types (Table 1). They are very easy to use: simply add the appropriate GenePusher Reagent to your culture medium 4 hours post-transfection. The level of gene expression can be enhanced by 2 to 12 fold (Table 3). On vial of GenePusher Reagent (1.5 ml), at a 100x concentration is sufficient for 150 transfections using 1 ml transfection volume.

The application of the GenePusher Reagent is cell type dependent. Indeed, some cells respond greatly to the GenePusher Reagent (see Table 1 and 3) whereas others are not responsive (Table 2).

      
  Table 1: Cell Types with improved transfection efficiency. GenePusher Reagents successfully increase the expression level of a β-galactosidase reporter gene in the cell lines listed
Transfected cell types
Jurkat K562
HeLa S3 MCF-7
PC-12 293
P19 HUV-EC-C

     
  Table 2: Cell types non-responding to the GenePusher Reagents. For some cell lines listed, GenePusher Reagents may exhibit some cytotoxicity.

Transfected cell types
B16-F0 CV-1
BHK-21 Hep-G2
CHO-K1 MDCK
COS-7 NIH-3T3

     
  Table 3: Recommended uses for the GenePusher and GeneTrans Reagents

Cell Type GenePusher
#1		 GenePusher
#2		 GenePusher
#3		 Recommended
Transfection
Reagent		 Enhanced
Jurkat ♦♦♦ NR NR GeneTrans 4.0 - 8.0 x
K-562 ♦♦♦ NR NR GeneTrans II 4.0 - 12.0 x
HeLa-S3 NR ♦♦♦ ♦♦ GeneTrans II 4.0 - 10.0 x
MCF-7 NR ♦♦ ♦♦ GeneTrans II 1.5 - 2.0 x
PC-12 NR ♦♦ ♦♦♦ GeneTrans II 2.0 - 8.0 x
293 ♦♦ ♦♦ ♦♦ GeneTrans II 2.0 - 3.0 x
P19 ♦♦ ♦♦♦ ♦ GeneTrans II 2.0 - 3.0 x
HUV-EC-C NR nr ♦ GeneTrans II 1.5 - 2.0 x
Legend: ♦ works well, ♦♦ works better, ♦♦♦ works best, NR: not recommended due to increased toxicity and lowered transfection efficiency


     
 

Order Information, Shipping & Storage

Please click on the respective order # for further details.
Order Information
Order # Description Amount
CB-TDT10666-01 GenePusher Reagent Kit (1.5 ml of each GenePusher Reagents #1, #2 and #3)
CB-TDT-10100-01 GenePusher Reagent #1 3 x 1.5 ml
CB-TDT-10200-01 GenePusher Reagent #2 3 x 1.5 ml
CB-TDT-10300-01 GenePusher Reagent #3 3 x 1.5 ml
shipped at RT; store at -20 °C or 4 °C; stable for at least 6 months


Related Products

Transfection Reagents
Order # Description Amount
0101B GeneTrans Transfection Reagent 75 reactions (0.75 ml)
0102B GeneTrans Transfection Reagent 150 reactions (1.5 ml)
0103B GeneTrans Transfection Reagent 750 reactions (5 x 1.5 ml)
0201B GeneTrans II Transfection Reagent 75 reactions (0.75 ml)
0202B GeneTrans II Transfection Reagent 150 reactions (1.5 ml)
0203B GeneTrans II Transfection Reagent 750 reactions (5 x 1.5 ml)


Order # Description Amount
VS-FLP10010 pcDNA3-vector, wt-EosFP, with mitochondrial targeting signal, lyophilized DNA 10 µg
VS-FLP10020 pcDNA3-vector, wt-EosFP, FLAG®-tagged, lyophilized DNA 10 µg
VS-FLP10030 pcDNA3-vector, td-EosFP, FLAG®-tagged, lyophilized DNA 10 µg

     
                     
                     
                 
 

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