Useful analytical tools for phage display systems and fusion protein expression

products | cell biology and antibodies | M13 Phage, His and GST Antibodies

Cell Biology and Antibodies

M13 Phage, His and GST Antibodies

Anti-g3p (pIII) Antibody

Features:

antibody to phage M13 coat protein g3p (pIII)
clone: 10C3
monoclonal mouse IgG

The anti-g3p antibody is a useful analytical tool for current phage display systems using the g3-protein of M13 for display of recombinant proteins such as antibodies, protein- or peptide-libraries and more. (See also our pSKAN Phagemid Display System).

Applications:

The antibody can be used in Western blots in order to analyze:

correct reading-frame fusions of g3p-fusion proteins
expression levels of g3p-fusion proteins in E. coli and on the tip of the phage
ratios of displayed g3p-proteins and g3p-fusion proteins on the phage-capsid
M13-infected E. coli strains

The epitope is also recognized under non-denaturing conditions. Therefore, the antibody can be used for:

ELISA detection of g3p-fusion proteins
purification of g3p-fusion proteins by affinity chromatography
efficient and rapid analysis of antigen binding characteristics of recombinant antibodies selected by phage display (microplate assay)

The Epitope:

The antigenic epitope of the anti-g3p antibody resides within amino acids 292 to 302 of the mature g3-protein and is therefore compatible with most of the phagemid vectors using different N-terminal deletions of the g3-protein.

Amino acid sequence of the epitope:

- A T D Y G A A I D G F -

Anti-hPSTI Antibody

Features:

antibody to human pancreatic secretory trypsin inhibitor domain (hPSTI)
recognizes native hPSTI (soluble as well as hPSTI in the pSKAN phagemid pIII-hPSTI fusion protein)
monoclonal mouse IgG (clone R XXIII)

The anti-hPSTI antibody was specifically developed for our pSKAN Phagemid Display System, but can also be useful in other applications related to the human pancreatic secretory trypsin inhibitor (hPSTI).

PAGE of pSKAN phages presenting different hPSTI-pIII fusion proteins followed by Western blot with anti-pIII (lane 1-5); M13K07 wild type phage as control (lane M13). Two bands are visible with pSKAN phages: one for pIII and one for the larger hybrid protein hPSTI-pIII. In M13 wild type phages only one band is detected resulting from pIII.

Anti-His and Anti-GST Antibodies

Features:

optimally suited for monitoring expression of His-tagged and GST-tagged fusion proteins
useful for immunoaffinity purification of fusion proteins possessing a (His)₆ or GST tag
source: rabbit, polyclonal
markedly increased sensitivity for (His)₆ and GST tags

Vectors for expression of fusion proteins possessing a (His)₆ tag or glutathione-S-transferase (GST) tag are commonly used for simple immunoaffinity purification of recombinant proteins produced in E. coli (e.g. by metal-chelate affinity chromatography). The MoBiTec anti-His-Tag and anti-GST-Tag antibodies are very useful in monitoring (His)₆ -Tag and GST-Tag fusion protein expression and purification and show a markedly increased sensitivity for (His)₆ tags and GST tags compared with other commercially available anti-His-Tag or anti-GST-Tag antibodies.

For a detailed data sheet as a pdf-file, please click on the product No.

ORDER INFORMATION, SHIPPING & STORAGE:
order#	description	amount
PSKAN2	anti-hPSTI antibody	100 µl
PSKAN3	anti-pIII (g3p) antibody	100 µl
HIST01	anti-His-Tag antibody	100 µl
GSTT01	anti-GST-Tag antibody	100 µl
shipped on blue ice; store at -20°C

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See also chapter 5 of our online catalog.