M13 Phage and Tag Antibodies

Anti-g3p (pIII) Antibody

Features:

• Antibody to phage M13 coat protein g3p (pIII)
  
• Clone: 10C3
  
• Monoclonal mouse IgG

The anti-g3p antibody is a useful analytical tool for current phage display systems using the g3-protein of M13 for display of recombinant proteins such as antibodies, protein- or peptide-libraries and more. (See also our pSKAN Phagemid Display System).

Applications:

The antibody can be used in Western blots in order to analyze:

• Correct reading-frame fusions of g3p-fusion proteins
• Expression levels of g3p-fusion proteins in E. coli and on the tip of the phage
• Ratios of displayed g3p-proteins and g3p-fusion proteins on the phage-capsid
  
• M13-infected E. coli strains
  

The epitope is also recognized under non-denaturing conditions. Therefore, the antibody can be used for:

• ELISA detection of g3p-fusion proteins
  
• Purification of g3p-fusion proteins by affinity chromatography
  
• Efficient and rapid analysis of antigen binding characteristics of recombinant antibodies selected by phage display (microplate assay)

The Epitope:

The antigenic epitope of the anti-g3p antibody resides within amino acids 292 to 302 of the mature g3-protein and is therefore compatible with most of the phagemid vectors using different N-terminal deletions of the g3-protein.

Anti-SPINK1 (hPSTI) Antibody

Features:

• Antibody to Serine protease inhibitor Kazal-type 1 (SPINK1; human pancreatic secretory trypsin inhibitor domain (hPSTI))
  
• Recognizes native hPSTI (soluble as well as hPSTI in the pSKAN phagemid pIII-hPSTI fusion protein)
  
• Monoclonal mouse IgG (clone R XXIII)
  
• Reports indicate a potential of SPINK1 as an extracellular therapeutic target in prostate cancer)
  

The anti-SPINK1 (hPSTI) antibody was specifically developed for our pSKAN Phagemid Display System, but can also be useful in other applications related to the human pancreatic secretory trypsin inhibitor (hPSTI).

"About 10 percent of prostate cancer patients are SPINK1-positive and strategies to block SPINK1 signaling may have utility in this subset of patients. These studies should stimulate the development of antibody-based therapies against SPINK1 or targeting of EGFR in SPINK1-positive cancer patients," says study author Bushra Ateeq, a research fellow at the U-M Medical School.

Anti-His and Anti-GST Antibodies

Features:

• Optimally suited for monitoring expression of His-tagged and GST-tagged fusion proteins
  
• Useful for immunoaffinity purification of fusion proteins possessing a (His)₆ or GST tag
  
• Source: rabbit, polyclonal
  
• Markedly increased sensitivity for (His)₆ and GST tags
  

Vectors for expression of fusion proteins possessing a (His)₆ tag or glutathione-S-transferase (GST) tag are commonly used for simple immunoaffinity purification of recombinant proteins produced in E. coli (e.g. by metal-chelate affinity chromatography). The MoBiTec anti-His-Tag and anti-GST-Tag antibodies are very useful in monitoring (His)₆ -Tag and GST-Tag fusion protein expression and purification and show a markedly increased sensitivity for (His)₆ tags and GST tags compared with other commercially available anti-His-Tag or anti-GST-Tag antibodies.