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Acella™ - Cloning and Expression in one Cell |
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It all happens in one cell: Go from ligation to expression faster than ever! |
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The newest innovation from Edge BioSystems, Acella™ chemically competent and electrocompetent cells, enables cloning and expression in the same cell. These cells combine the characteristics of a superior cloning strain with the elements of a regulated protein expression system, eliminating time-consuming multiple transformations - so you can go from ligation to expression faster than ever. |
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Features |
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High transformation efficiency: |
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Chemically competent: ≥ 2 x 108 cfu/μg |
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Electrocompetent: ≥ 1010 cfu/μg |
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High-quality plasmid DNA |
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Complete deletions of endA and recA genes eliminate degradation and recombination of DNA |
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Isolate plasmid DNA after only 3 hours of growth
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BL21 (DE3) strain derivatives ideal for expression of T7 promoter-driven constructs |
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Downloads
For data sheets with protocols please click on the respective order no. in the table below. |
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Order Information, Shipping & Storage
Please click on the respective order # for further details.
| Order Information |
| Order # |
Description |
Amount |
| 36795 |
Acella™ Chemically Competent Cells,
Tubes |
12 Tubes x 50 μl |
| 10773 |
Acella™ pLysS Chemically Competent Cells,
Tubes |
12 Tubes x 50 μl |
| 42649 |
Acella™ Electrocompetent Cells,
Tubes |
6Tubes x 100 μl |
| store at -80°C, shipped on dry ice. |
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© Edge BioSystems
Acella Chemically Competent and Electrocompetent Cells are trademarks of Edge BioSystems, Inc. |
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QuickCells Chemically Competent E. coli |
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Excellent transformation within just 3 minutes! |
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QuickCells Chemically Competent E. coli are specially optimized to achieve excellent transformation efficiencies with a novel 3-minute transformation protocol. In addition, with QuickCells, you can perform the heat shock step at 37 °C, eliminating the inconvenient 42 °C water bath. QuickCells Chemically Competent E. coli are great for routine cloning experiments. The quick and easy protocol also makes them ideal for high-throughput transformations. QuickCells Chemically Competent E. coli have been prepared using a unique procedure to allow robust performance under diverse conditions.
For different cloning vectors there are diverse QuickCells Chemically Competent E. coli strains available. For use with M13 cloning vectors QuickCells F’ are suitable. Furthermore QuickCells BL21(DE3) and QuickCells BL21(DE3)pLys for efficient transgene expression from T7 promoter-based vectors are available. Both strains contain the λDE3 lysogen.
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Features |
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Simple 3-minute protocol |
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High transformation efficiencies: up to 5 x 107 to 1 x 108 cfu/µg of supercoiled DNA are commonly obtained |
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No hour-long recovery step and no inconvenient 42 ºC water bath |
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Useful genotypes choices of E. coli for cloning or T7 promoter-based gene expression |
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Convenient and efficiency-preserving single-use packaging |
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RichCells Chemically Competent E. coli |
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Competent E. coli for the highest transformation efficiency of ligated DNA!
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Most commercially available competent cells are tested using pure plasmids and under optimized transformation conditions. When they are transformed with real-life ligation mixtures you frequently get mediocre transformation efficiencies. RichCells Chemically Competent E. coli are highly optimized for highest and most robust transformation performances under diverse conditions, even when using ligation mixtures.
RichCells F’ Chemically Competent E. coli are also available for use with M13 cloning vectors.
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Features |
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Tolerant to large volumes of ligation mix and undesirable chemicals |
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Transformation efficiencies using real-life ligation conditions up to 1 x 109 cfu/mg |
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Ideal for routine cloning needs and high-throughput experiments |
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Related Products: SpreadEasy Plating Beads, Single-Use Tubes and Dispenser Bottle |
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Specifically designed for convenient, safe and optimal plating efficiencies of bacterial cells on standard agar plates.
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Order Information, Shipping & Storage |
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Please click on the respective order # for further details. |
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| Order information |
| Order # |
Description |
Amount |
| CB-CEC30100-01 |
QuickCells Competent E. coli |
20 x 50 μl |
| CB-CEC30150-01 |
QuickCells F' Competent E. coli |
20 x 50 μl |
| CB-CEC50200-01 |
QuickCells BL21(DE3) Competent E. coli |
20 x 50 μl |
| CB-CEC50250-01 |
QuickCells BL21(DE3)pLysS Competent E. coli |
20 x 50 μl |
| CB-CEC70100-01 |
RichCells Chemically Competent E. coli Transformation Kit |
20 x 50 μl |
| CB-CEC70150-01 |
RichCells F`Chemically Competent E. coli Transformation Kit |
20 x 50 μl |
| CB-RMB90050-01* |
SpreadEasy Plating Beads Single-Use Tubes |
50 tubes |
| CB-RMB90010-01* |
SpreadEasy Plating Beads Dispenser Bottle |
1 bottle / 100 plates |
shipped frozen; store at -70 °C
QuickCells Transformation Buffer store at RT; SOC medium store at RT
all components are stable for 6 months when stored properly
*shipped at RT; store at RT |
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