Photobleaching profiles of cells stained with Alexa Fluor 488 or fluorescein conjugates of goat anti–mouse IgG antibody F(ab')2 fragment (A11017, F11021) were used to detect HEp-2 cells probed with human anti-nuclear antibodies. Samples were continuously illuminated and images were collected every 5 seconds with a cooled CCD camera. Normalized intensity data demonstrate the difference in photobleaching rates(pict.). © by Molecular Probes.
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