Alexa Fluor Dyes

Comparison of the photobleaching rates of the Alexa Fluor 488 and Alexa Fluor 546 dyes and the well-known fluorescein and Cy3 fluorophores. The cytoskeleton of bovine pulmonary artery endothelial cells (BPAEC) was labeled with (top series) Alexa Fluor 488 phalloidin (A12379) and mouse monoclonal anti–-tubulin antibody (A11126) in combination with Alexa Fluor 546 goat anti–mouse IgG antibody (A11003) or (bottom series) fluorescein phalloidin (F432) and the anti–-tubulin antibody in combination with a commercially available Cy3 goat anti–mouse IgG antibody. The pseudocolored images were taken at 30-second intervals (0, 30, 90, and 210 seconds of exposure). The images were acquired with bandpass filter sets appropriate for fluorescein and rhodamine. © by Molecular Probes.
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