Alexa Fluor Dyes

Comparison of the relative fluorescence of 7-amino-4-methylcoumarin-3-acetic acid (AMCA) streptavidin (

) and Alexa Fluor 350 streptavidin, a sulfonated AMCA derivative (S11249,

). Conjugate fluorescence is determined by measuring the fluorescence quantum yield of the conjugated dye relative to that of the free dye and multiplying by the number of fluorophores per protein. © by Molecular Probes.
Back to Alexa page