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| Fluorescence output from an Alexa Fluor 546 goat anti–mouse IgG antibody (dye:protein ratio = 5.7) and a commercially available Cy3 goat anti–mouse IgG antibody (dye:protein ratio = 3.8). Antibody concentrations were adjusted to give equal absorbance at the excitation wavelength (535 nm). The relative fluorescence quantum yield of Alexa Fluor 546 conjugates is higher than that of Cy3 conjugates, even at high dye:protein ratios that would typically result in self-quenching effects with most other protein-labeling dyes. Back to Alexa page |
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