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Mobicol "F" |
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The versatile small column with inserted filter for affinity chromatography!
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Features
- Convenient snap off plug
- From a very few to up to 700 µl
- Autoclavable at 110 °C for 10 min
- Let it drain by gravity flow or spin it
- Compatible with minifuges and 1.5 – 2 ml cups
- Additional cap with Luer-lock connection
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Free Mobicol “F” sample on request |
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The Mobicol “F” columns for affinity chromatography are compatible to the laboratory standard. With affinity matrix they are convenient for: |
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- Purification of peptides
- Purification of tagged proteins
- Purification of native proteins (using immobilized IgG, NTP-binding proteins, β-lactamase, Igase, peroxidase, erythropoietin, β-galactosidase)
- Fractionation and purification of antibodies (using protein A/G, immobilized epitopes, peptide matrix)
- Purification of glycoproteins, and carbohydrates
- Purification of fatty acids and fatty acid binding proteins
- Whole mount embryo in situ hybridization
A filter of 35 µm pore size is inserted and a separately available upper filter conveniently caps the resin bed. The columns can be closed tightly with a screw-on cap and a snap-off plug at the outlet. |
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Order Information
| order # |
description |
amount |
| M105035F |
Mobicol "F" with fixed outlet plug, inserted 35 µm filter and 2 different screw caps |
50 |
| M1050 |
Mobicol "F" with fixed outlet plug and 2 different screw caps, without filters |
50 |
| M1053 |
Mobicol "F" with fixed outlet plug and 1 closed screw cap, without filters |
50 |
| M2210 |
Filter (large) 10 µm pore size, for Mobicol M1002, M1003, M1050 & M1053 |
50 |
| M523515 |
Filter (large) 35 µm pore size, for Mobicol M 1002, M1003, M1050 & M1053 |
50 |
| M2290 |
Filter (large) 90 µm pore size, for Mobicol M1002, M1003, M1050 & M1053 |
50 |
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Related Products
The Mobicol "F" columns are available prefilled with immobilized proteases, nucleases, alk. phosphatase, ß-galactosidase or a compound of your choice on request.

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MobiSpin Column "F" |
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The universal spin column for centrifugation!
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Features
- Column with filters of 10 µm pore size
- Column volume is 700 µl
- Fill in the matrix of your choice
- Can be centrifuged in 1.5 ml or 2 ml tubes and fits all common centrifuges
- Autoclavable at 110 °C for 10 min
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The MobiSpin Column "F" is convenient for diverse applications. You can fill in your matrix of your choice and spin the column in every common centrifuge in 1.5 ml oder 2 ml cups. For prefilled MobiSpin Columns "F" please see the related products.
For example you can use MobiSpin Columns "F" with gel filtration matrix for:
- Removal of unicorporated dyes or dye terminators for purification of DNA after labeling reactions
- DNA purification prior to sequencing
- Removal of free nucleotides
- Oligonucleotide purification after synthesis
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The column can be tightly closed with the screw cap. Snap off the outlet plug for emptying the column and use the reverse side of the plug for closing. |
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Order Information
| order # |
description |
amount |
| M105010S |
MobiSpinColumn "F" with fixed outlet plug, inserted small 10 um filter and screw cap |
50 |
| M105210S |
MobiSpinColumn "F" with fixed outlet plug, inserted large 10 um filter and screw cap |
50 |
For further information please download our Mobicol-Brochure.
Related Products
The MobiSpin Columns "F" are available prefilled with: Sephadex® G50, Sephacryl® S-200, S-300 and S-400, or a compound of your choice on request.
| order # |
description |
amount |
| SCO500 |
MobiSpin Column "F" prefilled with Sephadex® G50 |
20 |
| SCO200 |
MobiSpin Column "F" prefilled with Sephacryl® S-200 |
20 |
| SCO300 |
MobiSpin Column "F" prefilled with Sephacryl® S-300 |
20 |
| SCO300 |
MobiSpin Column "F" prefilled with Sephacryl® S-400 |
20 |
(Sephadex® and Sephacryl® are registered trademarks of Amersham Biosciences, Inc.)
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Mobicol "classic": Centrifugable and Autoclavable 1 ml Columns |
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The Mobicol "classic" for affinity chromatography and centrifugation!
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Features
- Can be centrifuged in minifuges
- Autoclavable at 120°C (with filters at 110°C)
- Have Luer-lock connectors
- Are compatible with laboratory standard
- Smallest and largest volumes can be treated easily
- Luer adapters available for tubing
- Can be closed tightly with a screw cap and a plug at the outlet
- Actual volume is 800 µl
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Mobicol "classic" with two different lids (Luer-lock and screw cap), two filters and outlet plug. Actual volume: 800 µl. Scale: enlarged . |
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| Mobicol "classic": centrifugable 1 ml column (actual volume with small lower filter 800 µl); |
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Filters:
Filter of the pore sizes 10 µm, 35 µm or 90 µm are available separately for Mobicol "classic". The filters can be inserted easily with the help of a plastic tool, which is delivered with the columns. The upper large filter fits tightly over the whole length of the column body.
Empty spin columns:
Since Mobicol "classic" can be centrifuged in minifuges, they are also excellent as spin columns. Our empty spin columns come with screw-cap, inserted filter (10 µm) and bottom plug.
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Order Information
For a detailed data sheet as a pdf-file, please click on the order#.
Free Mobicol "classic" samples on request.
| order # |
description |
amount |
| M1002 |
Mobicol "Classic" with 2 different screw caps without filters |
50 |
| M1003 |
Mobicol "Classic" with 1 closed screw cap, without filters |
50 |
| M1002S |
Empty Spin Column with closed screw cap, inserted plug and large 10 µm filter |
100 |
| M2110 |
Filter (small) 10 µm pore size, for Mobicol M1002, M1003, M1050 & M1053 |
50 |
| M513515 |
Filter (small) 35 µm pore size, for Mobicol M 1002, M1003, M1050 & M1053 |
50 |
| M2190 |
Filter (small) 90 µm pore size, for Mobicol M1002, M1003, M1050 & M1053 |
50 |
| M2210 |
Filter (large) 10 µm pore size, for Mobicol M1002, M1003, M1050 & M1053 |
50 |
| M523515 |
Filter (large) 35 µm pore size, for Mobicol M 1002, M1003, M1050 & M1053 |
50 |
| M2290 |
Filter (large) 90 µm pore size, for Mobicol M1002, M1003, M1050 & M1053 |
50 |
| Filters have to be ordered separately. Two filter insertion tools (for small and large filters) are provided with each pack of columns. Lab column set and accessories. |
For further information please download our Mobicol-Brochure.
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Accessories |
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Variable Accessories are available for the Mobicols and Lab Columns: |
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| Fig. A Tubing-to-Luer-lock adapter. Scale: reduced |
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- Tubing-to-Luer-lock adapter for Mobicols or laboratory columns (Fig. A)
- Luer-adapter for the top-cap (M), Luer-adapter for outlet (F) and Luer-lock adapter (M)/(M) (Fig. B)
- One-way stopcocks, Luer-lock (Fig. C)
- Different small and large filters
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Fig. B Adapters for connecting tubing to
the columns. Luer-adapter for the top-cap
(M); Luer-adapter for outlet (F), Luer-lock-
adapter (M/M) |
Fig. C One-way stopcock |
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Order Information
| order # |
description |
amount |
| M3001 |
Tubing-to-Luer-lock adapter (material outside: metal; inside: glass and PTFE; with 1 m PTFE tubing) for Mobicols or laboratory columns |
1 |
| M3002 |
Luer-adapter, (M) and (F) 10 each |
20 |
| M3003 |
Luer-adapter for the top-cap (M) |
20 |
| M3004 |
Luer-adapter for outlet (F) |
20 |
| M3007 |
Luer-lock adapter (M)/(M) |
1 |
| M3005 |
Bottom plugs for M 1002 |
50 |
| M3006 |
Stopcocks, one-way, Luer-lock |
4 |
| M3009 |
Luer-lock caps |
50 |
For further information please download our Mobicol-Brochure.

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Related Products: Matrices
| order # |
description |
amount |
| AC-201-10-JB |
6% CL-Glutathione ChroMatrix(TM), Bulk material - 10 ml |
10 ml |
| AC-303-25-JB |
High Density Nickel Agarose, Bulk material - 25 ml |
25 ml |
| AC-304-25-JB |
Low Density Nickel Agarose, Bulk material - 25 ml |
25 ml |
| AC-305-25-JB |
High Density Zinc Agarose, Bulk material - 25 ml |
25 ml |
| AC-202-10-JB |
6% CL-Nickel ChroMatrix(TM), Bulk material - 10 ml |
10 ml |
| AC-309-5-JB |
Protein A Agarose, Bulk material - 5 ml |
5 ml |
| AC-101S-JB |
Immobilized gamma-Aminophenyl-ATP (C10-spacer), Bulk material - Agarose |
1 ml |
| AC-102S-JB |
Immobilized gamma-Aminophenyl-ATP (no spacer), Bulk material - Agarose |
1 ml |
| AC-106S-JB |
Immobilized gamma-Amino-octyl-GTP, Bulk material - Agarose |
1 ml |
| AC-107S-JB |
Immobilized gamma-Amino-octyl-UTP, Bulk material - Agarose |
1 ml |
| AC-108S-JB |
Immobilized gamma-Amino-octyl-CTP, Bulk material - Agarose |
1 ml |
| AC-109S-JB |
Immobilized gamma-Amino-octyl-ITP, Bulk material - Agarose |
1 ml |
| AC-110S-JB |
Immobilized gamma-Amino-octyl-XTP, Bulk material - Agarose |
1 ml |
| AC-111S-JB |
Immobilized gamma-Amino-octyl-dATP, Bulk material - Agarose |
1 ml |
| AC-115S-JB |
Immobilized gamma-Amino-octyl-dTTP, Bulk material - Agarose |
1 ml |
| AC-119S-JB |
Immobilized gamma-Amino-hexyl-CTP, Bulk material - Agarose |
1 ml |
| AC-120S-JB |
Immobilized gamma-Amino-hexyl-ITP, Bulk material - Agarose |
1 ml |
| AC-121S-JB |
Immobilized gamma-Amino-hexyl-XTP, Bulk material - Agarose |
1 ml |
| AC-122S-JB |
Immobilized gamma-Amino-hexyl-dATP, Bulk material - Agarose |
1 ml |
| AC-124S-JB |
Immobilized gamma-Amino-hexyl-dUTP, Bulk material - Agarose |
1 ml |
| AC-137S-JB |
Immobilized O-Phospho-L-Serine (C10-spacer), Bulk material - Agarose |
1 ml |
| AC-139S-JB |
Immobilized O-Phospho-L-Threonine (C10-spacer), Bulk material - Agarose |
1 ml |
| AC-103S-JB |
Immobilized O-Phospho-L-Tyrosine (C10-spacer), Bulk material - Agarose |
1 ml |
| AC-104S-JB |
Immobilized O-Phospho-L-Tyrosine (no spacer), Bulk material - Agarose |
1 ml |
| AC-142S-JB |
Immobilized 2'/3'-EDA-m7GTP, Bulk material - Agarose |
1 ml |
| AC-144S-JB |
Immobilized 2'/3'-EDA-ADP, Bulk material - Agarose |
1 ml |
| AC-145S-JB |
Immobilized N6-(6-Amino)hexyl-AMP, Bulk material - Agarose |
1 ml |
| AC-146S-JB |
Immobilized 8-Amino-hexyl-cAMP, Bulk material - Agarose |
1 ml |
| AC-147S-JB |
Immobilized 2'-EDA-cAMP, Bulk material - Agarose |
1 ml |
| AC-148S-JB |
Immobilized 2'-EDA-cGMP, Bulk material - Agarose |
1 ml |
For further information please download our Mobicol-Brochure.
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| ©2012 MoBiTec GmbH • All rights reserved • www.mobitec.com |
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