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MoBiTec - Product Highlights - NEWS |
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28.05.2010 MoBiTec |
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Click-Chemistry
 Click-chemistry was first introduced by K. Berry Sharpless in 2001. It describes a chemical reaction which builds substances by joining small units together. Click-reactions are fast and purposeful with high yields.
The most popular click-reaction is azide alkyne Huisgen cycloaddition with Copper (Cu) as catalyst at room temperature.
Now you can use this highly efficient technology for labeling of DNA or RNA via solid-phase synthesis or PCR. Furthermore, it is possible to introduce up to three different modifications onto one position of the same DNA strand. Of course, we offer different kinds of non-fluorescent and fluorescent labels.
For more information please visit our Click-Chemistry overview.
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03.03.2010 MoBiTec |
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New Vector Systems Brochure available from MoBiTec
Our new brochure focuses on gene expression in different microorganisms like Escherichia coli, Bacillus megaterium, Bacillus subtilis, Lactococcus lactis and yeast. Furthermore, it includes expression systems for mammalian cells as well as vector systems for many other purposes..
Content:
- NICE® Expression System for Lactococcus lactis
- Bacillus megaterium Expression Systems
- Bacillus subtilis Expression Vectors
- pBacTag Tagging Vectors
- Yeast Expression Vectors
- pORF-CLONE Vector System
- pPICHOLI Shuttle Vector System
- Mammalian Expression Vectors
- CMV Expression Vectors
- Fusion Protein Cloning Systems
- BRP Plasmids and Competent BRP Cells
- PCR Cloning Vector p3T
- Poly(His)-tag Cloning Vector pEG-His1
- Exontrap Cloning Vector
- ssDNA-Production and Expression in pMEX
- Promoter-Trap Vector pBBR RESO
- Broad-Host-Range Vectors pBBR122 and pBHR1
- Suicide Vector pCorrectClone
- Multiple Cloning Site Vector pMCS5
- Standard Cloning Vectors
- EosFP – Green to Red Photoconvertible Fluorescent Protein
- Related Products
Please download the new Vector Systems Brochure (PDF, 3879 KB) !
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29.01.2010 MoBiTec |
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High Yield Gene Expression with the new T7 RNA Polymerase Gene Expression System!
This system combines the benefits of the tightly regulated and strong T7 RNA polymerase gene expression system and alkaline protease free Bacillus megaterium.
The T7 RNAP gene expression system is tightly regulated and efficiently inducible through the xylA operon and the strong T7 RNAP promoter resulting in stable, high yield protein production up to 8-fold increased in comparison to common xylose inducible expression (Fig. 1).
The system is based on two parallel-replicating plasmids: pT7-RNAP and pPT7.
In addition to the t7 rnap gene under control of the strong xylA promoter pT7-RNAP contains the genes of ampicillin and chloramphenicol for easy selection in E. coli (AmpR) and B. megaterium (CmR).
pPT7 is responsible for the T7 RNAP-dependent expression of the target gene. Downstream of the T7 promoter it comprises a multiple cloning site with ten unique restriction enzyme cleavage sites. Additionally, the plasmid carries two resistances to ampicillin (in E. coli) and tetracycline (in B. megaterium).
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Fig. 1 Comparison of GFP amounts produced by variant expression systems. GFP produced by B. megaterium carrying the common xylose-regulated Plasmide pRBBm34 (square), B. megaterium transformed with the T7 expression system plasmids (circle) and B.megaterium with the T7 expression system plasmids treated with rifampicin. (Gamer et al., 2009) |
Pretransformed B. megaterium protoplasts!
For your convenience we offer B. megaterium protoplasts pretransformed with pT7-RNAP, so that you just have to insert your gene of interest into pPT7 and transform the pretransformed protoplasts with this plasmid.
| Order Information, Shipping & Storage |
| order # |
description |
amount |
| BMEGT702 |
Bacillus megaterium protoplasts, strain MS941, pretransformed with pT7-RNAP |
5 x 500 µl |
| BMEGT701 |
Bacillus megaterium high yield T7 gene expression kit, includes pretransformed protoplasts BMEGT702 (5 x 500µl), pPT7 cloning vector and pPT7-GFP control vector (vectors lyophilized, 10 µg each)
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kit |
| BMEGT710 |
Bacillus megaterium pPT7 cloning vector, lyophilized |
10 µg |
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07.08.2009 MoBiTec |
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Inositol Phosphates and Phosphoinositides
Inositol phosphates play important roles in diverse cellular functions, such as cell growth, apoptosis, cell migration, endocytosis, and cell differentiation. The group of inositol phosphates includes inositol monophosphate, inositol trisphosphate, and inositol pentakisphosphate. MoBiTec offers a hugh range of Inositole Phosphates, membrane permeant Inositol Phosphates, caged Inositol Phosphates and Phosphoinositides.
Read more... |
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16.07.2009 MoBiTec |
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Macromolecular Crystallography
Macromolecular Crystallography is a technique used to study biological molecules such as proteins, to a resolution higher than ~0.5 nm (~ 5 Å). This high resolution helps elucidate the complex mechanism by which these macromolecules carry out their functions in living cells and organisms. MoBiTec now offers Screens, plates and more: all you need for crystallization of biological macromolecules.
Read more...
Download the Macromolecular Crystallography brochure, to get an overview about the available products. (PDF 2MB) |
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05.01.2009 MoBiTec |
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NICE® Expression System for Lactococcus lactis
Controlled gene expression in L. lactis – an emerging alternative for recombinant protein production
Next to the wealth of traditional food applications, L. lactis is increasingly used for modern biotechnological applications such as the production of recombinant proteins for food, feed, pharma and biocatalysis applications. In addition to to the easy genetic accessibility of L. lactis the Nisin Controlled gene Expression (NICE®) system has played a key role in this development.
Features
- The system is fully food grade
- No endotoxins are produced
- No inclusion bodies
- No spores
- No extracellular proteinases
- Tightly controlled gene expression allows production of toxic proteins
- Simple fermentation, scale-up and down stream processing
Read more about the new NICE® Expression System for Lactococcus lactis |
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02.06.2008 MoBiTec |
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EosFP - Green to Red Photoconvertible Fluorescent Protein for Local Marking |
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Our portfolio of fluorescent proteins has been extended by a new fluorescent protein that allows UV/blue-inducible, permanent, bright and fast photoconversion from green to red wave length. These properties make it an excellent choice as a marker for tracking of cells, compartments or proteins in live cells, as well as a nanoscopy marker using photoactivated localization microscopy (PALM).
Read more
Download our new EosFP Flyer (PDF, 317 kb) or order a free paper Copy!.
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26.05.2008 MoBiTec |
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New brochure on Molecular Screening Technology available from MoBiTec |
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This 16-page brochure focuses on several yeast one- and two-hybrid systems and a phagemid system, that are useful for high-throughput molecular screening of protein-protein and DNA-protein interactions. Further tools, such as biochemical assays, immunological and fluorescence-based methods, are available from MoBiTec as well. The yeast two-hybrid system has rapidly become one of the most widely used techniques in molecular biology. It still is the method of choice to identify protein-protein interactions from either cDNA libraries or known gene sequences in vivo. The method relies on the transactivation of reporter genes in Saccharomyces cerevisiae identifying positive interactions.
Download the new Molecular Screening Brochure (1.2 MB) or order a free paper Copy!
Content:
Grow’n’Glow GFP Reporter Systems
MoBiTec’s Grow’n’Glow one- and two- hybrid systems are powerful tools for gene discovery and functional analysis. A GFP reporter allows one-step selection of positive clones under UV light.
Y2H Membrane Systems
MoBiTec’s split-ubiquitin based systems circumvent the restrictions that are usually associated with traditional Y2H systems, now allowing to detect interactors with integral membrane proteins, self-activating and transcriptionally active proteins.
- Y2H Membrane System
Versatile, reliable detection and analysis of specific binding partners of full-length integral membrane and membrane-associated proteins.
- Y2H Transactivating Protein System
Screens transcriptionally active and self-activating proteins that cannot be screened using classical Y2H systems.
Tools and Media
MoBiTec offers many tools optimized for working with Y2H and yeast: Kits to transform yeast cells, kits to purify plasmids from yeast, media, supplements and fermentation technology.
Phagemid System
- Phagemid Display System
Easy-to-handle, fast and low-cost approach for highthroughput and high-content screening of protein-protein interactions.
Download the new Molecular Screening Brochure (1.2 MB) |
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MoBiTec |
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Recombinant Nucleases |
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Extendend range of nucleases, e.g. engineered RNase TA, RNase T1, first recombinant RNase T2, and temperature-resistant Exonuclease III. Some of them are also available as immobilized enzymes on our proprietary Mobicol spin columns.
Read more
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MoBiTec |
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Magnetic Silica Beads |
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MoBiTec introduces a new product line of magnetic silica beads in the range from 150 nm up to 20 µm in diameter that are specifically designed for separation, concentration and purification of biomolecules, specific cells or cell compartments. They are ideally suited as a solid phase in assay kits. Due to a fast and flexible production process unrestricted customization possibilities with regard to sizes, kinetics, optical, surface and binding properties is facilitated.
Read more
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MoBiTec |
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DNA Labeling Kits |
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New product line for direct enzymatic incorporation of fluorescently labeled nucleotides into DNA by PCR, suitable for all typical DNA labeling applications such as FISH, microarray gene expression profiling, single molecule detection and other nucleic acid hybridization assays. The kits contain all reagents required for DNA labeling providing a highly efficient, easy-to-perform and rapid labeling procedure. The excellent stability and quantum yield of the fluorophores combined with a high incorporation rate of the dye-dUTP complex makes it the ideal choice for a broad range of fluorescence applications.
Read more
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MoBiTec |
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ImmunoSelect® Adhesion Slides |
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MoBiTec ImmunoSelect® adhesion slides are developed for microscopical use, where precious and only poorly available cellular material should be efficiently immobilized. In contrast to commonly coated slides the ImmunoSelect® adhesion slides stop cell loss even at harsh incubation procedures.
For more details please click here.
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MoBiTec |
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Bacillus subtilis Expression vectors |
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Gram-positive bacteria are well known for their contributions to agricultural, medical and food biotechnology and for the production of recombinant proteins. Among them, Bacillus subtilis has been developed as an attractive host because of several reasons. We can offer you exclusively some new B. subtilis Expression vecors.
For more details please click here.
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