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News and Highlights

EosFP - Green to Red Photoconvertible Fluorescent Protein for Local Marking

Our portfolio of fluorescent proteins has been extended by a new fluorescent protein that allows UV/blue-inducible, permanent, bright and fast photoconversion from green to red wave length. These properties make it an excellent choice as a marker for tracking of cells, compartments or proteins in live cells, as well as a nanoscopy marker using photoactivated localization microscopy (PALM).
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New brochure on Molecular Screening Technology

This 16-page brochure focuses on several yeast one- and two-hybrid systems and a phagemid system, that are useful for high-throughput molecular screening of protein-protein and DNA-protein interactions. Further tools, such as biochemical assays, immunological and fluorescence-based methods, are available from MoBiTec as well. The yeast two-hybrid system has rapidly become one of the most widely used techniques in molecular biology. It still is the method of choice to identify protein-protein interactions from either cDNA libraries or known gene sequences in vivo. The method relies on the transactivation of reporter genes in Saccharomyces cerevisiae identifying positive interactions.

Download the new Molecular Screening Brochure (1.2 MB).

Content:

Grow’n’Glow GFP Reporter Systems

MoBiTec’s Grow’n’Glow one- and two- hybrid systems are powerful tools for gene discovery and functional analysis. A GFP reporter allows one-step selection of positive clones under UV light.

• Grow’n’Glow GFP Two-Hybrid System
  Time-saving identification of positive clones with the well-established GFP reporter system, suitable for high-throughput screening assays. See page 2-3.
  
• Grow’n’Glow GFP One-Hybrid System
  Ideal to isolate genes of novel DNA-binding proteins or to map residues/motifs responsible for DNA-binding.
• Grow’n’Glow ACE1 Two-Hybrid System
  Stringent selection and more efficient detection of protein interactions using the copper-inducible CUP1/ACE1 system.

Y2H Membrane Systems

MoBiTec’s split-ubiquitin based systems circumvent the restrictions that are usually associated with traditional Y2H systems, now allowing to detect interactors with integral membrane proteins, self-activating and transcriptionally active proteins.

• Y2H Membrane System
  Versatile, reliable detection and analysis of specific binding partners of full-length integral membrane and membrane-associated proteins.
  
• Y2H Transactivating Protein System
  Screens transcriptionally active and self-activating proteins that cannot be screened using classical Y2H systems.

Tools and Media

MoBiTec offers many tools optimized for working with Y2H and yeast: Kits to transform yeast cells, kits to purify plasmids from yeast, media, supplements and fermentation technology.

• Yeast Transformation & Yeast Plasmid Purification Kits
• Yeast Media

Phagemid System

• Phagemid Display System
  Easy-to-handle, fast and low-cost approach for highthroughput and high-content screening of protein-protein interactions.

Download the new Molecular Screening Brochure (1.2 MB).

Recombinant Nucleases

Extendend range of nucleases, e.g. the first commercially available recombinant RNase A, engineered RNase TA, RNase T1, first recombinant RNase T2, and temperature-resistant Exonuclease III. Some of them are also available as immobilized enzymes on our proprietary Mobicol spin columns.
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Magnetic Silica Beads

MoBiTec introduces a new product line of magnetic silica beads in the range from 150 nm up to 20 µm in diameter that are specifically designed for separation, concentration and purification of biomolecules, specific cells or cell compartments. They are ideally suited as a solid phase in assay kits. Due to a fast and flexible production process unrestricted customization possibilities with regard to sizes, kinetics, optical, surface and binding properties is facilitated.
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DNA Labeling Kits

New product line for direct enzymatic incorporation of fluorescently labeled nucleotides into DNA by PCR, suitable for all typical DNA labeling applications such as FISH, microarray gene expression profiling, single molecule detection and other nucleic acid hybridization assays. The kits contain all reagents required for DNA labeling providing a highly efficient, easy-to-perform and rapid labeling procedure. The excellent stability and quantum yield of the fluorophores combined with a high incorporation rate of the dye-dUTP complex makes it the ideal choice for a broad range of fluorescence applications.
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ImmunoSelect^® Adhesion Slides

MoBiTec ImmunoSelect^® adhesion slides are developed for microscopical use, where precious and only poorly available cellular material should be efficiently immobilized. In contrast to commonly coated slides the ImmunoSelect^® adhesion slides stop cell loss even at harsh incubation procedures.

For more details please klick here.

Bacillus subtilis Expression vectors

Gram-positive bacteria are well known for their contributions to agricultural, medical and food biotechnology and for the production of recombinant proteins. Among them, Bacillus subtilis has been developed as an attractive host because of several reasons. We can offer you exclusively some new B. subtilis Expression vecors.

For more details please klick here.

Kinases and Protein Tyrosine Phosphatases available

MoBiTec presents a new product line of Kinases and Protein Tyrosine Phosphatases. These enzymes are very usefull for the study of enzyme kinetics or regulation issues. Moreover they can be used to dephosphorylate target substrates or for screening inhibitors.

We offer a broad range of different Kinases and Tyrosine Phosphatases with different catalytic domains.

for information about the phosphatases please click here
for information about the kinases please click here

We represent in Europe: Echelon Biosciences, Inc.

Echelon offering a broad range of products for lipid cell signalling, e.g. antibodies, enzymes, assays, isoprenoids, lysophospolipid & sphingolipid products, MEP pathway intermediates, phospho-inositides & derivatives, phospholipase substrates etc. They are very useful for development of potential drugs and diagnostics for cancer, diabetes, inflammation, infections, and cardiovascular disease.

For details click here

We represent in Europe: GenTel Biosurfaces, Inc.

providing state-of-the-art tools for protein arrays, e.g. kits, slides and reagents for making own protein microarrays.

For details click here

We represent in Europe: Evident Technologies, Inc.

Evident Technologies is the leading manufacturer of quantum dot technology. Their EviTags are semiconductor nanocrystals with excellent properties emitting a long-lasting, very bright fluorescence (in the range of 465-2300 nm) being excited with a singular wavelength. They are superior to many known fluorophores and are suited for labelling of biomolecules. Protein labeling kits are available as well.

For details click here

New Y2H Membrane Protein System

Easy detection and characterization of proteins interacting with membrane proteins

Applications

• Identify novel interaction partners of your membrane protein of interest by library screening
  
• Confirm the dimerization of known membrane proteins
  
• Define the critical amino acids of an interacting domain
  
• Generate comprehensive maps of membrane protein interactions
  

Advantages

• Efficient in vivo screening for specific binding partners of full-length integral membrane and membrane-associated proteins
  
• Posttranslational modifications such as glycosylation and disulfide bond formation are preserved
  
• Protein-protein interactions are identified in their natural compartment - the membrane
  

For details click here

New improved Bacillus megaterium Expression System: pHIS1522 / pHIS1525 and pMM1522 & pMM1525

• stable protein expression with high yield
  
• easy purification and detection of 6 x His-tagged target proteins
  
• xylose operon: tightly regulated and efficiently inducible by xylose (up to 350-fold)
  
• extended polylinker downstream of promoter allows versatile cloning
  
• additional BsrGI site enables cloning without modifying the N-terminus
  
• no indication of proteolytic instability even up to 5 hours after induction
• endotoxins are not found in the cell wall
  
• suited for industrial large scale protein production
  
• all B. subtilis vectors are compatible
  
• pHIS1525 provides signal peptide sequence for efficient protein secretion
  

For more information click here

Fluorescent MFP Dyes - Superior Tools for Fluorescent Imaging

Amine reactive MFP dyes, Labeled streptavidin, MFP Protein Labeling Kits,...

Our MFP dye range is growing continuously!

For details click here

MFP Labeled Secondary Antibodies

We are offering now high- quality labeled antibodies with excellent price / performance ratio

MFP-Dyes are the cost-effective alternative to currently excisting well-kown fluorophores such as the Cy^TM labels.

For details click here

AdnaTest Cancer

• Test system for the selection and detection of cancer cells from human blood
• optimal for the detection of disseminated tumor cells - available for breast and colon tumors (AdnaTest Breast and AdnaTest Colon)
  
• high analytical sensitivity (2 cells in 5 ml of blood) and a high specificity achieved through combination of multi-marker tumor cell
  
• enrichment and gene expression profiling
  
• turn around time of approximately 8 hours

pPEPTIDE Cloning Vector

• highly efficient expression of recombinant target proteins and very small polypeptides
  
• even toxic proteins are overexpressed after induction
  
• significantly improved Poly(His)-Tag allows optimal purification of the fusion protein using metal affinity chromatography
  
• easy chemical release of the target peptide/protein
  
• cost-effective, time saving method to produce labeled and non-labeled peptides in adequate yields

MoBiTec DNA Ladder

Intelligent marker for PCR product determination (bands between 1500 - 500 bp). After short gel running time (8-9 mm) three DNA bands are visible at 1500, 1000 and 500 bp. Further bands are visible after long gel running time!

250 bp Ladder

Universal marker for plasmid and PCR product size determination (bands between 8000 - 250 bp).

For details click here

Grow'n'Glow GFP Yeast One-Hybrid System

Improved sensitivity!
Now available with positive controls!

For details click here

  Grown´n´Glow ACE1 Two-Hybrid-System

	efficient detection of protein-protein interactions in vivo by the copper-inducible CUP1/ACE1 system
	fewer false-positives by stringent selection for copper-resistance
	identifies binding regions of proteins known to interact and allows to select for different protein-protein interaction strengths
	faster screening for positives using the GFP reporter -positives are fluorescing brightly green
	suited for the development of HTS assays

For details click here

MiniCeM™ - Miniaturized Cell Chamber

	allows high-resolution, non-invasive microscopical measurements and observations - ideal for sterile micro-manipulation and long-term observations of single cells
	sterile and simple transfer of living cells and tissue as well as medium and chemicals into the chamber is possible
	cell cultures can grow properly in the miniature chamber
	simple integration of miniaturized pH and temperature probes

For details click here

MobiGLOW Mounting Medium

	stabilizes fluorochromes and decreases photobleaching
	higher contrast of the fluorescence images
	clearly prolonged gleaming signals
	conserves the cells and fixes the cover slide
	ready-to-use

For details click here

Jenchrom™ px blue Detection Kit

	dark blue reaction product
	high detection sensitivity
	fluorescence visualization is possible with pulsed NIR lasers at 800 nm
	applicable for studies on living cells

For details click here

ProTrans Protein Delivery Reagent

For details click here

GeneTrans Gene Delivery Reagents

For details click here

Anti-Tet-Repressor Antibody

Polyclonal and monoclonal anti-TetR antibodies:
	excellent binding properties to TetR
	new tool for studies of tet regulatory system in eukaryotic cells
	suited for Western blots and ELISA
	monoclonal antibodies are offered in an optimized mix consisting of specific antibodies which are obtained from different cell lines

For details click here

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