- optimized for two- and one-hybrid screenings
- most media come in bags for 0.5 liter medium
Our media formulations are supplied as ready-to-autoclave powder mixtures. Most of them come in small bags with the appropriate amount of powder for 0.5 liter medium. You only add water and autoclave for use.
Grow'n'Glow yeast media are optimized for two- and one-hybrid studies and strongly recommended for our Grow'n'Glow Systems to achieve best results. We offer a complete standard Wickerham yeast nitrogen base with carbon source optimized for S. cerevisiae that can be added to water and autoclaved without the need to make concentrated solutions of vitamins, trace elements, salts, or carbon sources. This powder drop out base formulation is called DOB Medium, or DOBA with agar. A complete supplemented synthetic defined medium is easily made by mixing two powders, DOB (or DOBA) and CSM (Complete Supplement Mixture).
Grow'n'Glow Yeast Media:
||20 g peptone, 10 g yeast extract, 20 g glucose per liter, pH 6.5 at 30°C (rich medium)
||YPD with 17 g agar per liter
||26.7 g/liter (1.7 g YNB, 5 g ammonium sulphate, 20 g glucose)
||DOB (glu) with 17 g agar per liter
|DOB (2% gal/1% raf):
||36.7 g/liter (1.7 g YNB, 5 g ammonium sulphate, 20 g galactose, 10 g raffinose)
|DOBA (2% gal/1% raf):
||DOB (gal/raf) with 17 g agar per liter
CSM - Complete Supplement Mixture
The formulation of CSM is a dropout supplement for virtually all strains of S.cerevisiae containing different combinations of amino acids, adenine and uracil. Cells grow vigorously in DOB or DOBA supplemented with CSM.
For use with our Grow'n'Glow Systems we offer:***
CSM -HIS -LEU -TRP -URA supplement
CSM -HIS -TRP -URA supplement
CSM -HIS -URA supplement
CSM -HIS -LEU supplement
CSM -HIS supplement
CSM -TRP -URA supplement
CSM -URA supplement
SD - Synthetic Defined Yeast Media
Synthetic defined yeast media includes all necessary compounds for selective growth of Saccharomyces cerevisiae. We offer synthetic defined broth (SD) suitable for our y2h membrane protein system.
SD = DOB + Appropriate CSM
Grow'n'Glow Bacterial Media:
||10 g tryptone, 5 g yeast extract, 10 g NaCl per liter
||LB medium with 15 g agar per liter