Frequently used genomic libraries with automatic subcloning facility

products | genomiclibraries | Eukaryotic LambdaPS Libraries

Genomic Libraries

Eukaryotic LambdaPS Libraries

Features and Advantages:

large insert size (up to 20 kb)
convenience of working with high copy-plasmids
complete representation of the genome (at least 5-fold)
automatic subcloning facility
competitive price (especially for kits of several libraries!)
of special interest: the pufferfish model vertebrate genome

Applications:

screening
easy characterization of clones
homology studies
pufferfish as model for discovery of human genes
gene targeting (mouse library: strain 129, congenic with popular ES cell lines)
developmental, evolutionary and genetic studies on vertebrates and non-vertebrates

Characterization of LambdaPS Libraries:
species	average insert size	redundancy (about)	suggested # of phage per plating
human (male; peripheral blood leukocytes)	17.5 kb	20	2 x 10 ⁶
mouse (129/Sv, male, embryonal stem cell line)	17 kb	4	2 x 10 ⁶
rat (Sprague-Dawley)	18 kb	10	2 x 10 ⁶
zebrafish (Danio rerio)	17 kb	5	2 x 10 ⁶
pufferfish (Fugu rubripes)	17 kb	8	2 x 10 ⁵
*D. melanogaste*r (Canton S)	20 kb	500	2 x 10 ⁵
*C. elegans*	17 kb	50	2 x 10 ⁵
*S. cerevisiae* (YNN295)	17.5 kb	10	2 x 10 ⁴

Product Description:

In comparison to other library systems, genomic libraries created in the vector lambdaPS facilitate screening and subsequent characterization of clones significantly. Since the replacement vector of phage origin can accommodate up to 20 kb DNA inserts, complex genomes are completely represented in a reasonable number of recombinants (approx. 5-fold coverage). For characterization of selected clones the elegant system features an automatic plasmid subcloning facility of insert fragments: linear lambdaPS phage contains two loxP sites in direct orientation flanking a high copy plasmid backbone and the insert. Recombination between these two sites is mediated by Cre recombinase (in the re-expressing E. coli strain BNN132). This leads to excision of the multi-copy plasmid from the phage genome obviating the need for subcloning the insert into another system. Thus, time-consuming purification of large amounts of phage DNA for characterization is now superfluous. A restriction map is easily compiled using the excised plasmid, which can be further manipulated.
Of special interest to the researcher should be the library from the pufferfish genome (Fugu rubripes), which is 7.5 times smaller than the human genome. Because of its similar gene repertoire it is the best model for the discovery of human genes. The lambdaPS phage for construction of custom made libraries is also available for the experienced researcher.

Automatic subcloning: Site-specific recombination between loxP sites catalysed by Cre-recombinase from E. coli BNN132.

ORDER INFORMATION, SHIPPING STORAGE:
order#	description	amount
1 ml phage lysate each; host strains included:
PS01	1. human genomic lambdaPS library	1 ml
PS02	2. mouse genomic lambdaPS library	1 ml
PS03	3. rat genomic lambdaPS library	1 ml
PS04	4. zebrafish genomic lambdaPS library	1 ml
PS05	5. pufferfish genomic lambda PS library	1 ml
PS06	6. C. elegans genomic lambdaPS library	1 ml
PS07	7. D. melanogaster genomic lambdaPS library	1 ml
PS08	8. yeast genomic lambdaPS library	1 ml
PS15	Vertebrate kit (#1-5)	1 ml each
PS68	Non-vertebrate kit (#6-8)	1 ml each
PS18	Complete kit (#1-8)	1 ml each
PS10	lambdaPS phage lysate	1 ml
shipped on dry ice; store at -70°C

See also chapter 3 of our online catalog.

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