Tools for Cell Culture

Features:

• Allows high-resolution, non-invasive microscopical measurements and observations
• Sterile and simple transfer of living cells and tissue as well as medium and chemicals into the chamber is possible by using standard medical syringes and needles
• Cell cultures can grow properly in the miniature chamber
• Ideal for sterile micro-manipulation with laser tweezers and long-term observations of single cells
• Simple integration of miniaturized pH and temperature probes

Product Description:

The miniaturized cell chamber for microscopy (MiniCeM™) consists of a round plastic fitting and a cover (with MiniCeM™ logo) as well as an exchangeable inner chamber. The actual volume of the inner chamber consists of a flexible silicon ring and two round cover slips each 170 µm thick. The two components of the chamber are connected by a bayonet fitting that enables a rapid change of the internal chamber set.

	outer chamber diameter:	65 mm
	observation surface:	300 - 700 mm2
	chamber volume:	approx. 3 ml
	silicone ring:	translucent, non-toxic, gas-permeable
	material of the chamber proper:	glass-fibre reinforced polypropylene
	glass window for observation:	diameter 40 mm, 0.17 mm thick borosilicate glass (suitable for fluorescence as well as transmission)
	CLSM*: tested for	Zeiss LSM 510, 710 ; Leica TCS SP2; Nikon A1R-A1; Olympus FV 1000, 500, 300

The flexible material of the inner chamber wall facilitates the exchange of gases and permits insertion of sterile medical needles.
The entire cell chamber is made of autoclaveable material. Due to its miniaturized dimensions, the cell chamber is suitable for both upright as well as inverted microscopes and can be used for transmission and reflection measurements, conventional, confocal and multi-photon fluorescence imaging, as well as optical micro-manipulations with laser tweezers. These can be accomplished because of the very thin cover slips (170 µm) with low autofluorescence and objectives with high numerical apertures for oil and water immersion.

It is possible to place the cell chamber on the microscope mounting plates intended for fixing Petri-dishes with a diameter of 65 mm. For efficient handling of numerous samples, special racks (MiniCeM™-Racks) for 5 or 10 cell chambers are also available. These can be used in the incubators or temperature controlled chambers.

Applications:

The cell chamber MiniCeM™ is designed for microscopical applications in the cell biology, biotechnology and medicine. In particular, spatial high-resolution observations of living cells are possible over an extended period of time. The MiniCeM™is perfectly suited for CLSM application an was tested with the following microscopes:

• Zeiss LSM 710, LSM 510
• Leica TCS SP2
• Nikon A1R-A1
• Olympus FV 1000, 500, 300
  

For facilitated and precise relocation of single cells in long-term experiments!

Order Information, Shipping & Storage

Downloads: Handbook

Also available:

• MiraCLEAN
  Highly improved convenient method for the removal
  of bacterial endotoxins from nucleic acids.
  
  For detailed information click here
  
  
  
• Antibiotics
  For selection and maintenance of pro- and eukaryotic
  cells - available in different product forms
  Hygromycin B, G418 (Geneticin^® ), Puromycin,
  Cycloheximid, Mycophenolic Acid, Gentamycin
  Sulphate.
  
  For detailed information click here
  
  
  
• Beta-Gal Staining Kit
  Fast and simple determination of transfection efficiency
  
  
  • Efficient and reliable method for assaying beta-galactosidase
    expression in situ following transient or stable transfections
    as well as in tissue sections following in vivo transfections
    
  • Complete kit for direct colorimetric visualization of
    individual cells expressing the reporter gene
    
    
  For detailed information click here

Lift-Off Detaching Reagent

The Lift-Off Detaching Reagent contains protease, and collagenase activities in an isotonic phosphate buffer solution with EDTA. Lift-Off detaching reagent provides quick and gentle detachment and dissociation of a wide variety of adherent cells, including primary cells, grown in all known tissue culture plastic ware.
The Lift-Off Detaching Reagent is suitable for detaching adherent cells from in vitro growth vesicles. Lift-Off detaching reagent offers the following benefits:

• Gentle and rapid detachment
  
• Maximum cell viability
  
• Effective on a wide range of cells
  
• No mammalian or bacterial by-products
  
• Stable at 4 °C for 2 months
  
• No need to wash detached cells

Order Information, Shipping & Storage