Products | Fluorescence Technology

DNA-labeling Kits

MoBiTec - Innovative Tools for Molecular Biology

Whole chromosome painting of chromosome 21. Fluorescent labeling of WCP21 by Atto550

Proprietary linker technology provides fluorescent labeled dUTP analogs for incorporation into DNA at highest rates

MoBiTec introduces ready-to-use kits for direct enzymatic incorporation of fluorescently labeled nucleotides into DNA by PCR, suitable for all typical DNA labeling applications such as FISH, microarray gene expression profiling, single molecule detection and other nucleic acid hybridization assays.
The Kits contain all reagents required for DNA labeling providing a highly efficient, easy-to-perform and rapid labeling procedure. The excellent stability and quantum yield of the fluorophores combined with a high incorporation rate of the dye-dUTP complex makes it the ideal choice for a broad range of fluorescence applications.
In PCR labeling reactions dTTP is partially substituted by dye-dUTP. These dye-nucleotides are efficiently incorporated by Taq polymerase resulting in fluorescently labeled double-stranded DNA.

Each kit provides you with:

Ready-to-use labeling mix
Taq polymerase
Optimized reaction buffer
PCR-grade water

accompanied by a streamlined documentation that maximizes success.

Note: All labeled nucleotides are also available separately!

Emission Color	Product	Absorption max. [nm]	Emission max. [nm]	To be used instead of	Cat.-No.
blue-green	Atto425 PCR Labeling Kit	436	484	DEAC	FT-LNT10010-01
green	Atto488 PCR Labeling Kit	501	523	Alexa Fluor 488, Fluorescein (FITC), FAM, Oregon Green 514, Rhodamine green, Rhodamine 123	FT-LNT10020-01
yellow	Atto550 PCR Labeling Kit	554	576	Alexa Fluor 555, Cy 3, Alexa Fluor 546, TAMRA, Rhodamine Red, Spectrum Orange	FT-LNT10030-01
orange	TexasRed PCR Labeling Kit	583	603	Rhodamine ITC, Cy 3.5, ROX, Alexa Fluor 568	FT-LNT10040-01
orange	Atto590 PCR Labeling Kit	594	624	Alexa Fluor 594, Alexa Fluor 610	FT-LNT10050-01
red	Atto647N PCR Labeling Kit	644	669	Alexa Fluor 647, Cy 5	FT-LNT10060-01
far-red	Atto655 PCR Labeling Kit	663	684	Alexa Fluor 660, Cy 5.5	FT-LNT10070-01

Click on the respective order # to download a detailed data sheet.

Order Information
order #	description	amount
FT-LNT10010-01	Atto425 PCR Labeling Kit	10 reactions
FT-LNT10010-05	Atto425 PCR Labeling Kit	50 reactions
FT-LNT10020-01	Atto488 PCR Labeling Kit	10 reactions
FT-LNT10020-05	Atto488 PCR Labeling Kit	50 reactions
FT-LNT10030-01	Atto550 PCR Labeling Kit	10 reactions
FT-LNT10030-05	Atto550 PCR Labeling Kit	50 reactions
FT-LNT10040-01	TexasRed PCR Labeling Kit	10 reactions
FT-LNT10040-05	TexasRed PCR Labeling Kit	50 reactions
FT-LNT10050-01	Atto590 PCR Labeling Kit	10 reactions
FT-LNT10050-05	Atto590 PCR Labeling Kit	50 reactions
FT-LNT10060-01	Atto647N PCR Labeling Kit	10 reactions
FT-LNT10060-05	Atto647N PCR Labeling Kit	50 reactions
FT-LNT10070-01	Atto655 PCR Labeling Kit	10 reactions
FT-LNT10070-05	Atto655 PCR Labeling Kit	50 reactions