Macromolecular Crystallography

JBS Halo-Hg Nucleotides

Halogenated and mercurated nucleotides for phasing applications with nucleotide- or DNA-binding proteins. 

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JBS Halo Kits

Selections of halogenated ATP or GTP derivatives in single kits will enable you to screen them all in parallel. Don't waste time and crystals with a conventional "trial and error" heavy atom screen - get all the material you need for MIR or MAD experiments from one 24 well tray!

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JBS Methylation

The JBS Methylation Kit is designed for selective methylation of lysine residues. The method does not require laborious cloning/expression/purification but chemically replaces the protons of the amino group of all lysine residues with methyl groups. The result is a surface-engineered protein within 24 hours ready for crystallization.

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JBS Tantalum Cluster Kits

Tantalum Bromide Cluster for MIR and MAD phasing.

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JBScreen Basic

Based on the classic sparse matrix crystallization screen published first by Jancarik and Kim in 1991, it contains 96 unique reagent mixtures for screening a wide range of pH and various salts and precipitants.

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JBScreen Buffer Kits

JBScreen Buffer Kits are designed for convenient reproduction and optimization of crystallization conditions. The solutions can be used to reformulate conditions of the JBScreen family, e.g. JBScreen Classic, JBScreen Basic, JBScreen Cryo, and other commercially available crystallization screens.

Furthermore, JBScreen Buffer Kits can be employed for the straightforward preparation of custom screen solutions for the refinement and optimization of initial crystallization conditions. The JBScreen Buffer Kit formulations will help to save time preparing accurate and high-quality reagents for the reproducible production of single protein crystals.

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JBScreen Single Stocks

Single stock solutions of the JBScreen components, i.e. polymers, buffers and salts are ideal for the optimization of your crystallization conditions.

Using the same chemicals as utilized in the JBScreens ensures higher reproducibility of your experiments. JBScreen Single Stocks are ready for use: the concentration is adjusted and they are sterile filtered.

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JBScreen Classic

The JBScreen Classic Kits 1-10 cover 240 (10 kits x 24 conditions each) of the most prominent conditions for protein crystallization. Their compositions result from mining of literature data of several thousands of crystallized proteins. JBScreen represents the selected statistically most successful buffers that yielded protein crystals suitable for X-ray diffraction.

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JBScreen Cryo

The advanced cryo screen available in 10 ml vials or 96-well deep well plates.

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JBScreen Cryo Pro

JBScreen Cryo Pro is the most convenient tool on the market for producing effective cryoprotectants from your crystallization reservoir solution. The kit contains 12 different compounds, divided into sugar/aminoacid-based cryoprotectants, alcohol-based cryoprotectants, and an oil-based cryoprotectant.

The predispensed samples of each solid and 50 µl of each liquid formulation are ready to be diluted with the reservoir solution. Crystals soaked in this resevoir solution/cryoprotectant mixture can be directly transfered to a liquid nitrogen bath or cryogenic gas stream.

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JBScreen Detergents

The JBScreen Detergents Kits 1 and 2 cover all detergents that have been successfully used for the crystallization of membrane proteins. Each kit contains 12 detergents, supplied as stock solutions at 5 or 10 times the reported CMC (Critical Micellar Concentration), with 100 or 200 µl per compound.

The JBScreen Detergents Kits are ideal supplements for the JBScreen Membrane screens. This combination will enable you to screen a broad range of crystallization conditions, while concentrating on the most successful detergents - and therefore making crystallization screening of membrane proteins much more efficient and less time consuming.

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JBScreen JCSG++

Optimized sparse matrix screen developed by the Joint Center for Structural Genomics (JCSG).

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JBScreen Kinase

A highly effective crystallization screen based on data analyses of published kinase structures.

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JBScreen Membrane

The optimized screen for hydrophobic and membrane proteins.

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JBScreen PACT++

Systematic screen for pH, anion, cation testing in the presence of polyehtylene glycol.

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JBScreen PEG-Salt

Efficient screening of PEG 3350 and PEG 5000 MME versus 48 different salts.

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JBScreen pH-2D

JBScreen pH-2D is designed to sample a broad pH-range without changing the buffering environment. This ensures pH-screening independent of the chemical nature of the components of the buffer system.

JBScreen pH-2D contains six extended range buffer systems; each composed of a mixture of three individual buffers with a distinct chemical nature and well separated pKa values. Each buffer system is composed of a low-pH and a high-pH stock solution, supplied in 10 ml volumes. The low-pH stock solution is preset at pH 4.0 and the high-pH solution at either pH 9.0 or 10.0 (see download). Thus, mixtures of the low-pH and high-pH stock solutions in different proportions allow to cover the entire pH range from 4.0 – 9.0 or 4.0 – 10.0, respectively. The composition of the buffer systems assures a virtually linear distribution of the resulting pH vs the percentage of high pH / low pH stock.

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JBScreen Phosphatase

Optimized sparse matrix screen based on data of published phosphatase structures.

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JBScreen Plus

JBScreen Plus is an additive screen most useful in the optimization of preliminary crystallization conditions. The selection of the additives is based on the Hofmeister series, which reflects the ability of ions to stabilize the structure of proteins. Thus ions can be classified as either kosmotropic or chaotropic. The first having structure stabilizing properties, thus they may assist in, e.g. crystallizing proteins with a high proportion of flexible loop regions. The latter show structure disturbing properties which may assist in the crystallization of large complexes allowing them to re-arrange to form favorable crystal contacts.

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JBS Solubility Kit

The JBS Solubility Kit is a pre-crystallization screen to improve the composition of the initial protein buffer solution prior to performing crystallization set-ups. Since the highly complex properties of proteins are dependent on their environment, buffer solutions play an important role, i.e. influencing the solubility and the aggregation behaviour of the protein sample. Studies have shown that aggregation of the protein may inhibit nucleation and crystal growth. Therefore, the JBS Solubility Kit has been developed to investigate protein samples towards their homogeneity and monodispersity prior to crystallization trials, employing hanging drop vapour diffusion experiments combined with dynamic light scattering. The JBS Solubility Kit contains a set of 24 buffer solutions at different pH-values for setting up hanging drop vapour diffusion experiments in order to monitor the aggregation and precipitation of the protein sample, and a set of 14 additives used for further optimization employing dynamic light scattering.

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