products | Cell Biology and Antibodies | MFP Dyes

Cell Biology and Antibodies

	Amine Reactive MFP
	Thiol Reactive Dye
	MFP Dye Labeled Streptavidin, R-Phycoerythrin
	MFP Protein Labeling Kits
	MFP Secondary Antibodies

Please download MFP dye brochure (pdf, 1.8 MB)

MFP dyes

The newly developed MFP dyes are an ideal and cost-effective alternative to currently existing well-known fluorophores such as the Cy^TM labels. They can be readily used to label biomolecules (e.g. antibodies, peptides or nucleic acids) very efficiently. They are of exceptional high quality and performance.

Figure 1: The cytoskeleton in HEp2 cells has been detected using mouse monoclonal β-tubulin antibody, visualized with MFP488 goat anti-mouse (A-C) and with a well-known competitive goat anti-mouse (D-F). Nuclei were labeled using TO-PRO-3®. A,D: secondary antibody concentration 1:250; B,E: 1:250 after bleaching (100% light intensitiy for 30 seconds); C,F: 1:500.

MFP dyes have several advantages over competing brands including

• Brightness - MFP dyes exhibit more brightness than the commonly used dyes
  
• High emission fluorescence - MFP dyes have optimal light emission due to high quantum yield values
  
• High fluorescence intensity - MFP dyes exhibit very intense fluorescence resulting from the high extinction coefficients and quantum yields of the dyes
  
• High photostability - MFP dyes are more photostable than most of the commonly used fluorescent dyes. You have more time for observation and image capture.
  
• High water solubility - MFP dyes are highly water soluble allowing easy protein labeling procedures without the use of organic solvents
  
• pH insensitivity - MFP dyes remain highly fluorescent over a broad pH range (pH 4 - 9)
  
• Instrument compatibility - Excitation and emission spectra of the MFP dyes are compatible with the commonly used excitation sources and filter sets

Figure 2: Relative fluorescence of MFP488 goat anti-mouse conjugates at various dye/protein ratios (excitation at 500 nm).

The MFP dye series

MFP488 is compatible with green emitting fluorophores such as Cy^TM2 and fluorescein. MFP488 is brighter than fluorescein, much more photostable, water soluble and pH insensitive over a broad range (pH 4-9). Exc./em. maximum: 501/523 nm.

MFP555 is the perfect choice if you want to replace the red fluorescent Cy^TM3 dyes. Antibodies labeled with this dye are optimally suited for TMR and Cy^TM3 filters. Exc./em. maximum: 547/572 nm.

Figure 3: Absorption and fluorescence emission spectra of MFP488 and MFP555 dye in ethanol. Absorption and emission maxima shift to 501/523 nm for MFP488 and to 560/585 nm for MFP555 (measuring MFP488 goat anti-mouse IgG antibody and MFP555 goat anti-mouse IgG antibody in PBS buffer, pH 7.2).

MFP590 conjugates produce bright red fluorescence emission. Antibodies labeled with this dye are optimally suited for Texas Red^® filters. Exc./em. maximum: 597/624 nm.

MFP631 can be optimally excited with the 633 nm spectral line of the He-Ne laser and the 635 nm line of red diode lasers. The bright and photostable MFP631 emits in the far red region of the spectrum. Exc./em. maximum: 637/658 nm.

Figure 4: Absorption and fluorescence emission spectra of MFP590 and MFP631 dye in ethanol. Absorption and emission maxima shift to 597/624 nm for MFP590 and to 633/658 nm for MFP631 (measuring MFP590 goat anti-mouse IgG antibody and MFP631 goat anti-mouse IgG antibody in PBS buffer, pH 7.2).

Labeled Streptavidin

Streptavidin is a bacterially derived biotin-binding protein that reportedly exhibits less nonspecific binding than avidin. Streptavidin, a nonglycosylated 52,800-dalton protein with a near-neutral isoelectric point, reportedly exhibits less nonspecific binding than avidin. However, streptavidin contains the tripeptide sequence Arg-Tyr-Asp (RYD) that apparently mimics the Arg-Gly-Asp (RGD) binding sequence of fibronectin. We offer streptavidin labled with R-phycoerythrin (SAPE) and allophycocyanin (APC).

R-Phycoerythrin is an intensely bright phycobiliprotein with absorption maxima at approximately 496, 546 and 565 nm, and a fluorescence emission maximum of ~578 nm. Allophycocyanin is an intensely bright phycobiliprotein with a broad absorption profile that peaks at ~650 nm, and a fluorescence emission maximum of ~660 nm.

Labeled R-Phycoerythrin (R-PE), see order box below.

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MFP Protein Labeling Kits

For efficient protein or antibody labeling we offer the ready-to-use MFP Protein Labeling Kits. These new protein labeling kits provide a convenient tool for labeling your specific antibody or desired protein with our MFP dyes:

Advantages

• Convenient - Very simple protocol
  
• Ready-to-use - All buffers and purification columns are included - no equilibration necessary
  
• Fast - Time saving procedure
  
• Quality control - Premeasured reactive dyes
  
• Advantage of commonly used protein labeling kits - Spin columns for buffer exchange and protein concentration are included!
  

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We are also offer an extending range of MFP dye labeled secondary antibodies.

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