Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

Products

MagSi-gDNA Blood Kit

Fast and Economic Extraction of Genomic DNA

Blood donation
Gel electrophoresis of genomic DNA

Gel electrophoresis of genomic DNA extracted from 50-200 µL fresh whole blood

UV-VIS Absorbance spectrum of DNA

UV-VIS Absorbance spectrum of DNA isolated from 100, 150 and 200 µL fresh whole blood

Features

  • Suitable for whole blood, prepared with citrate, EDTA or heparin
  • Ultra pure, ready to use genomic DNA for enhanced performance in sensitive applications
  • Erythrocyte lysis is not necessary
  • High binding capacity
  • Reproducible, high yield of DNA – up to 8 µg from 200 µL fresh or frozen whole blood
  • No cross contaminations
  • Standardized processing ensures reproducible results
  • Optimal preservation of gDNA
  • Low amounts of (chemical) waste
  • Ordering of single different kit components possible
  • Easy to automate
    1. Compatible with many different automated liquid handling systems (e.g Thermo Scientific™ KingFisher™, PrimaDiag PrimaRWS®, Gilson pipetmaX®, Eppendorf epMotion®, Tecan Freedom EVO®, Hamilton MICROLAB® STAR Line/NIMBUS, PerkinElmer JANUS®)
    2. Fast and robust protocols
    3. Suitable for small, medium and high-throughput automation
    4. Large volume sample input possible

Description

  • The MagSi-gDNA blood kit allows fast and economic automated purification of genomic DNA from a wide range of sample volumes (20 µl up to 1 ml) of fresh or frozen whole blood samples using magnetic beads and any liquid handling workstation. The scalability of the MagSi-gDNA blood kit makes it possible to use the product in any situation where gDNA isolation from blood is needed: from single experiments using the magnetic separators to high-throughput experiments. For high-throughput platforms the KingFisher® is a perfect and widely used example.

Applications

  • PCR, Real-time PCR
  • RFLP analysis
  • Restriction enzyme digestion
  • Hybridization
  • SNP analysis
  • Genotyping
Product Starting Material Average Yield Preparation Time
MagSi-gDNA blood kit Up to 200 µl whole blood 3 - 15 µg, depending on the blood sample (storage and source) A260/A280: 1.8-2.0 Approx. 20-25 min. after lysis
  • In combination with the liquid handling systems the automated MagSi gDNA blood kits enables purification of DNA from 1 - 96 samples of whole human blood or other commonly used blood components (buffy coat, serum). After filling the standard containers (single tubes, 96 deepwell plates), all required isolation steps are robottically performed. In contrast to other blood kits available using blood volumes > 1 ml, lysis of erythrocytes is not required. Blood sample lysed with the MagSi gDNA blood kit is optimal as the lysis buffer contains Proteinase K.
    In combination with the robotic workstation the lysis step can be carried out direct on the platform at ambient temperature or via the integrated thermomixer available for most robots at 56 °C. Further processing is done by making use of the magnetic capabillities of the MagSi DNA beads.
    The free DNA binds to magnetic particles, followed by washing steps and the final elution. The high quality DNA can be stored at –20 °C or is ready to use for subsequent downstream applications.

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
MD60001 MagSi-gDNA blood kit 96 preps 210,00 PDF
MD61001 MagSi-gDNA blood kit 10 x 96 preps 1575,00 PDF
MD62001 MagSi-gDNA blood kit 10 x 960 preps (bulk) on request PDF
  • All prices are in EURO excl. VAT and shipping. For further pricing and order information please ask your local distributor.

Related Products

Order# Description Amount Price Data Sheet
PR-MAGMS01 Magnetic Separator M12+12 for the isolation of M-Beads Magnetic silica beads in 12 x 1.5 ml and 12 x 2 ml tubes 1 each 335,00 PDF
PR-MAGMS05P MM Separator 96 PCR 1 each 1313,00 PDF
  • All prices are in EURO excl. VAT and shipping. For further pricing and order information please ask your local distributor.

Download

  • MagSi-gDNA Blood Kit Product Leaflet (PDF)
  • Magnetic Beads Brochure (PDF)