Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

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Sequencing Clean-Up

Performa® DTR – Dye Terminator Removal System

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Performa DTR

Performa® DTR – Dye Terminator Removal Systems

Big Dye Terminator Removal with Performa® DTR

Features

  • Long read lengths – consistently achieve contiguous read lengths >900 bases in >95%
  • More readable sequence – identify bases close to the primer with excellent signal strength
  • Quick 2-spin protocol – start to finish in only 5 minutes
  • Minimal DNA manipulation – just add your sample and spin
  • Ready-to-use – pre-packed with a fully hydrated gel filtration matrix
  • Flexible formats – single, 96- and 384-well formats
  • Convenient – spin directly into the ABI Prism® 96-well Optical Reaction Plate or comparable plates and load directly onto capillary sequencers
  • Effective dye terminator removal of various chemistries – BigDye® v3.1, BigDye v1.1, GenomeLab™, DTCS Quick Start Kit and Methods Development Kit

Description

  • Performa® DTR utilizes a fully hydrated gel filtration matrix that provides a convenient and simple method of dye terminator removal for CE sequencing clean-up. Performa DTR ensures removal of BigDye® v3.1 or other dye terminators, along with dNTPs, salts and other low molecular weight materials from sequencing reactions. DNA primers and fragments up to 20 bases, buffers, nucleotides labeled with biotin, isotopes and other assorted markers are also eliminated. To minimize the potential for interference with sequencing applications, no preservatives, salts or buffers are used in the preparation of Performa DTR.

Downstream Applications

  • DNA Sequencing
  • SNP Genotypng

Performa® DTR Individual Cartridge Systems

Performa DTR Individual Cartridge Systems

Performa® DTR Individual Cartridge Systems

Column Flowchart

Column Flowchart
At the end of the procedure the sample is ready for immediate loading onto the capillary sequencing platform

Description

  • Performa DTR gel filtration cartridges are 800 µL spin columns assembled in 1.5 mL microcentrifuge tubes. These columns provide optimal performance for removal of unincorporated BigDye® v1.1, v3.0, and v3.1 and other dye terminators, dNTPs, salts, and other low molecular weight materials from sequencing reactions. These columns also remove DNA primers and fragments up to 20 bases, buffers, and nucleotides labeled with biotin, isotopes, and other assorted markers.


  • The spin columns are pre-packed with a fully hydrated matrix to afford optimal handling and performance characteristics. To minimize the potential for interference with sequencing applications, no preservatives, salts, or buffers are used in the preparation of these columns.

Shortest Time to Completion

  • Ready-to-Use: pre-packed with a fully hydrated gel filtration matrix
  • For a total reaction volume of 5-20 µl
  • 5 minutes total protocol time
  • Simple protocol with minimal handling
  • Only 3 steps!

Performa® DTR Multi-Well Systems

Performa DTR Ultra 96-Well Plate

Performa DTR Ultra 96-Well Plates
For a total reaction volume of 5-20 µl; for special sequencing protocols requiring high Dye Terminator volumes (also available as kit including lids and receiver plates, which can be purchased separately as well)

Performa DTR V3 96-Well Short Plate

Performa DTR V3 96-Well Short Plates
For a total reaction volume of 5-15 µl (also available as kit including lids and receiver plates, which can be purchased separately as well)

Performa DTR 384-Well Plate

Performa DTR 384-Well Plates
For a total reaction volume of 5-10 µl

Performa DTR Plates Flowchart

Plates Flowchart
Collect purified products directly into ABI Prism MicroAmp® Optical Reaction Plate or comparable plates, load directly onto your capillary sequencing platform.

Overview

  • The plates are available in three formats:

Shortest Time to Completion

  • Ready-to-Use: Pre-packed with a fully hydrated gel filtration matrix
  • Simplest protocol with minimal handling
  • Only 3 steps!

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
98780 Performa® DTR Gel Filtration Cartridges 36/pk 100,00 PDF
42453 Performa® DTR Gel Filtration Cartridges 108/pk 219,00 PDF
54789 Performa® DTR Ultra 96-Well Plate Kit 2 Plates 253,00 PDF
55373 Performa® DTR Ultra 96-Well Plate Kit 10 Plates 979,00 PDF
74251 Performa® DTR Ultra 96-Well Plate Kit (No Receivers) 10 Plates 923,00 PDF
86077 Performa® DTR Ultra 96-Well Plate Kit (No Receivers) 50 Plates 3560,00 PDF
63887 Performa® DTR V3 96-Well Short Plate Kit 2 Plates 239,00 PDF
89939 Performa® DTR V3 96-Well Short Plate Kit 10 Plates 901,00 PDF
47938 Performa® DTR V3 96-Well Short Plate Kit (No Receivers) 10 Plates 863,00 PDF
80808 Performa® DTR V3 96-Well Short Plate Kit (No Receivers) 50 Plates 3297,00 PDF
52571 Performa® DTR 384-Well Plates (No Receivers) 2 Plates 345,00 PDF
90636 Performa® DTR 384-Well Plates (No Receivers) 10 Plates 1328,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in Germany.
  • Please contact us for free samples!

Download

  • EdgeBio Performa DTR Systems Flyer (PDF)