Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

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TransIT®-VirusGEN™ Transfection Reagent

Ideal for Recombinant Adeno-Associated Virus Production

Virus Illustration
TransIT®-VirusGEN™ Outperforms Competitor Reagents in Suspension & Adherent AAV Cell Cultures

TransIT®-VirusGEN™ Outperforms Competitor Reagents in Suspension & Adherent AAV Cell Cultures
Suspension FreeStyle™ 293-F cells grown in FreeStyle™ F17 Medium (A) or adherent 293T/17 cells (B) were transfected with pAAV-hrGFP, pAAV-RC, and pAAV-Helper (1:1:1 ratio, 1.5 µg/ml, Agilent Technologies) with the following reagents: TransIT®-VirusGEN™ (2:1, vol:wt), Lipofectamine® 2000 (3:1, Thermo Fisher), Lipofectamine® 3000 (3:3:1, Thermo Fisher) or 25 kDa PEI (4:1, Polysciences). Harvested virus was used to transduce HT1080 cells and GFP expression was measured 48 hours post-transduction using guava easyCyte™ 5HT Flow Cytometer. Functional titers were measured from virus dilutions with less than 20% GFP positive cells. The error bars represent the standard deviation of triplicate wells.

TransIT®-VirusGEN™ Outperforms Competitor Reagents in Suspension and Adherent Lentivirus Cell Culturess

TransIT®-VirusGEN™ Outperforms Competitor Reagents in Suspension and Adherent Lentivirus Cell Cultures
Suspension FreeStyle™ 293-F cells grown in FreeStyle™ F17 Medium (A) or adherent 293T/17 cells (B) were transfected with pLKO. 1-puro-CMV-TurboGFP™ transfer vector and the Lentivirus Packaging Mix powered by MISSION® (1:1 ratio, 1 µg/ml) with the following reagents: TransIT®-VirusGEN™ (3:1, vol:wt), Lipofectamine® 2000 (2:1 (A) or 3:1 (B), Thermo Fisher), Lipofectamine® 3000 (2:2:1 (A) or 3:3:1 (B), Thermo Fisher) or 25 kDa PEI (4:1, Polysciences). The supernatant was harvested, filtered (0.45 µm), and titered using 293T/17 cells. Lentivirus transductions were performed in the presence of 8 µg/ml TransduceIT™ and GFP expression was measured 72 hours post-transduction using guava easyCyte™ 5HT Flow Cytometer. Functional titers were measured from virus dilutions with less than 20% GFP positive cells. The error bars represent the standard deviation of triplicate wells.

TransIT®-VirusGEN™ Enables Broad Scalability

TransIT®-VirusGEN™ Enables Broad Scalability
Suspension FreeStyle™ 293-F cells grown in FreeStyle™ F17 Medium (A) or adherent 293T/17 cells (B) were transfected with pAAV-hrGFP, pAAV-RC, and pAAV-Helper (1:1:1 ratio, 1.5 total µg/ml, Agilent Technologies) using the TransIT®-VirusGEN™ (2:1 reagent-to-DNA ratio (vol:wt), Mirus) using the indicated volumes and culture vessel. Harvested virus was used to transduce HT1080 cells and GFP expression was measured 48 hours by flow cytometry. Functional titers were measured from virus dilutions with less than 20% GFP positive cells. The error bars represent the standard deviation of triplicate wells, flasks, or dishes.

Features

  • Reliable – Consistent high virus titer production
  • Scalable – Efficient across different formats
  • Flexible – Address different virus and cell culture systems

Description

  • TransIT®-VirusGEN™ Transfection Reagent is designed to enhance delivery of packaging and transfer vectors to adherent and suspension HEK 293T cell types and increase recombinant adeno-associated virus (AAV) and lentivirus production.

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
MIR6703 TransIT®-VirusGEN™ Transfection Reagent 0.3 ml 126,00 PDF
MIR6704 TransIT®-VirusGEN™ Transfection Reagent 0.75 ml 315,00 PDF
MIR6700 TransIT®-VirusGEN™ Transfection Reagent 1.5 ml 499,00 PDF
MIR6705 TransIT®-VirusGEN™ Transfection Reagent 5 x 1.5 ml 2169,00 PDF
MIR6706 TransIT®-VirusGEN™ Transfection Reagent 10 x 1.5 ml 3990,00 PDF
MIR6710 TransIT®-VirusGEN™ Transfection Reagent 0.1 ml sample 0,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in selected countries.