Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

Products

TurboLigation™ Kit

Quick and Cost-Effective DNA Ligation

TurboLigation™ Kit

Features

  • Quick ligation in 5 minutes
  • Very competitive pricing
  • Ready for downstream applications without any further treatment

Description

  • Our TurboLigation™ Kit enables quick ligation of both blunt-end or cohesive-end DNA fragments in just 5 minutes at room temperature. The kit combines TurboLigase™, an ultrapure recombinant T4 DNA Ligase, and 5x TurboLigation buffer, an optimized quick ligation buffer. A 5 minute TurboLigation reaction offers the same efficiency as a traditional 16 °C overnight ligation reaction. The reaction product is ready for bacterial transformation without any further treatment. There is no need to heat-inactivate the TurboLigase, dilute the reaction, or purify the DNA before chemical transformation.

Application

  • Adding linkers or adapters to DNA
  • Blunt or cohesive end ligation of duplex DNA
  • DNA cloning

Components

  • TurboLigase and 5x TurboLigation Buffer, specially formulated for high efficiency ligation.

Unit definition

  • 0.015 Weiss units (1 cohesive end ligation unit) is defined as the amount of enzyme required to give 50% ligation of HindIII fragments of λ DNA (5´ DNA termini concentration of 0.15 µM) in a total reaction volume of 20 µl in 30 minutes at 16 °C in a buffer of 50 mM Tris-HCl, pH 7.5, 10 mM MgCl2, 1 mM ATP, 10 mM dithiothreitol.

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
GE-TLK0110-1 TurboLigation™ Kit 100 reactions 178,00 PDF
GE-TLK0110-2 TurboLigation™ Kit 500 reactions 681,00 PDF
  • All prices are in EURO excl. VAT and shipping. For further pricing and order information please ask your local distributor.