Quick and Cost-Effective DNA Ligation
- Quick ligation in 5 minutes
- Very competitive pricing
- Ready for downstream applications without any further treatment
- Our TurboLigation™ Kit enables quick ligation of both blunt-end or cohesive-end DNA fragments in just 5 minutes at room temperature. The kit combines TurboLigase™, an ultrapure recombinant T4 DNA Ligase, and 5x TurboLigation buffer, an optimized quick ligation buffer. A 5 minute TurboLigation reaction offers the same efficiency as a traditional 16 °C overnight ligation reaction. The reaction product is ready for bacterial transformation without any further treatment. There is no need to heat-inactivate the TurboLigase, dilute the reaction, or purify the DNA before chemical transformation.
- Adding linkers or adapters to DNA
- Blunt or cohesive end ligation of duplex DNA
- DNA cloning
- TurboLigase and 5x TurboLigation Buffer, specially formulated for high efficiency ligation.
- 0.015 Weiss units (1 cohesive end ligation unit) is defined as the amount of enzyme required to give 50% ligation of HindIII fragments of λ DNA (5´ DNA termini concentration of 0.15 µM) in a total reaction volume of 20 µl in 30 minutes at 16 °C in a buffer of 50 mM Tris-HCl, pH 7.5, 10 mM MgCl2, 1 mM ATP, 10 mM dithiothreitol.
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- All prices are in EURO excl. VAT and shipping. For further pricing and order information please ask your local distributor.