Minute™ Cytoplasmic & Nuclear Extraction Kit
Minute™ Cytoplasmic and Nuclear Fractionation Kit (order# SC-003)
Lane 1 and 2: 293T cell cytosol and nuclear fractions (name-brand kit); Lane 3 and 4: cell cytosol and nuclear fractions (Minute™ Cytoplasmic & Nuclear Extraction Kit, #SC-003)
- Invent Biotechnologies Minute™ Cytoplasmic and Nuclear Extraction Kit is composed of optimized cytoplamic extraction buffer, nuclear protein extraction buffer and protein extraction filter cartridges with 2.0 ml collection tubes. The kit is designed to rapidly separate native cytosol and nuclear proteins from cultured mammalian cells or tissues. Due to the use of the protein extraction filter cartridges, separation of cytolasmic and nuclear proteins can be accomplished in less than 15 minutes.
- The kit is designed to rapidly extract native cytoplasmic and nuclear proteins from cultured cells or tissues for applications such as SDS-PAGE, immunoblottings, ELISA, IP, protein localization, gel mobility shift assays, 2-D gels and other applications. This kit provides the most rapid method currently available for fractionation of native cytoplasmic and nuclear proteins.
|Cytoplasmic Extraction Buffer||25 ml||SC-003-L|
|Nuclear Extraction Buffer||25 ml||SC-003-N|
|Protein extraction filter cartridges||50||P-001|
|Collection tubes with cap||50||P-002|