Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

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Protein Extraction

Minute™ Detergent-Free Plant Protein Extraction Kit

Minute Detergent-Free Plant Protein Extraction Kit

MinuteTM Detergent-Free Plant Protein Extraction Kit (order# SN-010)

Comparison of plant protein profiles

Comparison of plant protein profiles extracted by denaturing lysis buffer and detergent-free extraction buffer.
Lane 1: Corn kernel; Lane 2: Broccoli leaves; Lane 3: Spinach leaves. Extracted proteins were separated in 10% SDS-PAGE and stained with coomassie blue.

Description

  • Invent Biotechnologies' Minute™ detergent-free plant protein extraction kit is composed of optimized protein extraction buffer and protein extraction filter cartridges with 2.0 ml collection tubes. The kit is designed to rapidly extract detergent-free total proteins from plant tissues. The protein extraction buffer does not contain any detergent and organic solvent. Due to the use of the protein extraction filter cartridges, the extraction volume can be as small as 50 µl and as large as 500 µl. Detergent-free total proteins can be extracted from very small plant samples (leaves, seeds, soft stems etc.) in less than 8 min with high yield (1-6 mg/ml).

Application

  • Minute™ detergent-free plant protein extraction kit is designed to rapidly extract water soluble proteins from plant tissues for applications such as proteomics (LC/MS), IP, ELISA, 2D-gel analysis, isoelectric focusing, SDS-PAGE, immunoblottings, and other applications. This kit provides a very rapid method for preparation of high concentration of protein extract.

Kit Components:

Description Amount Part #
DF-lysis buffer (with 5 g beads) 25 ml SN-010-L
Protein extraction filter cartridges 50 P-001
Collection tubes with cap 50 P-002
Plastic rods 4 P-003

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
SN-010 Minute TM Detergent-Free Plant Protein Extraction Kit (50 Tests) 50 Tests 240,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in Europe.