Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

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Protein Extraction

Minute™ Detergent-Free Protein Extraction Kit for Animal Cultured Cells and Tissues

Minute™ Detergent-Free Total Protein Extraction Kit for Animal Cells/Tissues

Minute™ Detergent-Free Total Protein Extraction Kit for Animal Cells/Tissues (order# SN-006)

Comparison of protein profiles of different animal tissues

Comparison of protein profiles of different animal tissues extracted by denaturing lysis buffer and detergent-free extraction buffer.
Lane 1: Drosophila cultured cells (SL2); Lane 2: Drosophila adult flies; Lane 3: gold fish muscle; Lane 4: mouse liver. Extracted proteins were separated in 10% SDS-PAGE and stained with coomassie blue.

Description

  • Invent Biotechnologies' Minute™ detergent-free total protein extraction kit is composed of optimized cell lysis buffer and protein extraction filter cartridges with 2.0 ml collection tubes. The kit is designed to rapidly extract detergent-free total proteins from cultured cells (insect /mammalian/other cultured cells) and animal tissues (invertebrate and vertebrate). The protein extraction buffers do not contain any detergent and EDTA. Due to the use of the protein extraction filter cartridges, the extraction volume can be as small as 20 µl and as large as 500 µl. This unique feature is very useful in situations where available starting material is a limiting factor. Detergent-free total proteins can be extracted from cultured cells/tissues in less than 5 min with high yield (1-5 mg/ml).

Application

  • Minute™ detergent-free total protein extraction kit is designed to rapidly extract total proteins from invertebrate and vertebrate cultured cells/tussues for applications such as proteomics (LC/MS), IP, ELISA, 2D-gel analysis, isoelectric focusing, SDS-PAGE, immunoblottings, and other applications. This kit provides a very rapid method for preparation of high concentration of whole cell extract. Because of high yield of protein extract and low salt concentration, the protein extract can be used directly for downstream applications without desalting and protein concentration.

Kit Components:

Description Amount Part #
Buffer A 15 ml SN-006-A
Buffer B 15 ml SN-006-B
Protein extraction filter cartridges 50 P-001
Collection tubes with cap 50 P-002
Plastic rods 4 P-003

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
SN-006 Minute TM Detergent-Free Total Protein Extraction Kit for Animal Cells/Tissues (50 Tests) 50 Tests 288,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in Europe.