Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

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Protein Extraction

Minute™ Detergent-Free Protein Extraction Kit for Microbes with Thick Cell Walls

Minute Detergent-Free Protein Extraction Kit

MinuteTM Detergent-Free Protein Extraction Kit for Microbes with Thick Cells Walls (order# YD-016)

SDS-PAGE profiles of extracted proteins by detergent-free extraction buffers

SDS-PAGE (12%) profiles of extracted proteins by detergent-free extraction buffers.
Lane 1: S. cerevisiae
Lane 2: L. lactis (Gram+)

Description

  • Protein extraction from cell wall-containing microbes is a frequent procedure in bio-research Labs. The methods for protein extraction from these microbes are usually harsh, tedious and not sufficiently reliable. Minute™ Detergent-Free Protein Extraction Kit for Microbes with Thick Cell Walls (#YD-016) provides a detergent- free, rapid and gentle way for extracting proteins from microbes with thick and strong cell walls. These microbes include but not limited to yeast, filamentous fungus, gram positive and negative bacteria, insect eggs and microalgae. This kit contains optimized detergent-free and EDTA-free protein extraction buffers. The whole procedure takes less than 10 min to complete and the protein yield is in the range of 2-4 mg/ml with un-biased protein representation. This kit features a single tube protocol and works well for both log phase and stationary phase microbes. The materials provided are sufficient for 50 extractions.

Application

  • Proteins extracted with this kit can be used for many downstream applications such as SDS-PAGE analysis, Western blotting, IP. ELISA and enzyme activity assays and proteomic analysis. The buffers are compatible with IMAC resins for his-tagged protein purification.

Kit Components:

Description Amount
Buffer A 20 ml
Buffer B 20 ml
Protein extraction powder 5 g
1.5 ml microfuge tube 50
Pestles 4

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
YD-016 Minute™ Detergent-Free Protein Extraction Kit for Microbes with Thick Cell Walls (50 Tests) 50 Tests 145,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in Europe. For further pricing and order information please ask your local distributor.