Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

Products

MobiTEV Protease

Improved TEV Protease at an Affordable Price

Cleavage site of MobiTEV Protease

Schematic view of cleavage site

Features

  • Highly specific cleavage activity
  • Robust activity over a broad temperature (4 °C to 37 °C) and pH (7 to 9) range
  • Enhanced enzyme stability for prolonged protease activity
  • Greater than 85% single-band purity with no non-specific protease contamination
  • Easy removal of MobiTEV Protease from the digested protein sample using the polyhistidine

Description

  • TEV Protease is used to remove fusion tags - solubility, secretion, detection, and purification tags - and release the "native" protein of interest. MobiTEV Protease is an improved version of Tobacco Etch Virus (TEV) protease that is highly site-specific, highly active, and significantly more stable than native TEV Protease, resulting in enhanced long-term activity. MobiTEV Protease specifically recognizes a seven amino acid sequence (Glu-Asn-Leu-Tyr-Phe-Gln-Gly, cleaving between Gln and Gly).

Unit Definition

  • One unit of MobiTEV Protease cleaves 3 µg of the control substrate at >85% in 1 hour in 30 µl reaction buffer (50 mM Tris/HCl, 0.5 mM EDTA and 1mM DTT) at pH 8.0 and 30 °C.

Formulation and Storage

FormulationStorage
1000 U MobiTEV Protease (2 U/µl) is formulated in 50 mM Tris/HCl pH 7.5, 1 mM EDTA, 5 mM DTT, 70 mM imidazol, 50% (v/v) glycerol. Store at -80 °C. Avoid repeated freeze-thaw cycles. Stable for 6 months when properly stored.
1 ml 20x MobiTEV Reaction Buffer, Tris-HCl pH 8.0, 10 mM EDTAStore at 4 °C
500 µl 0.1 M DTT in deionized waterStore at -20 °C

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
PR-ETA10010-01 MobiTEV Protease, recombinant, His-Tag 1000 U 161,00 PDF
PR-ETA10010-05 MobiTEV Protease, recombinant, His-Tag 10 x 1000 U 959,00 PDF
  • All prices are in EURO excl. VAT and shipping. For further pricing and order information please ask your local distributor.

Related Products: TEV Protease with additional GST-tag

Order# Description Amount Price Data Sheet
PR-ETA10050-01 TEV Protease(TurboTEV), recombinant, GST- & His-Tag 10 µg (100 U) 35,00 PDF
PR-ETA10050-02 TEV Protease(TurboTEV), recombinant, GST- & His-Tag 50 µg (500 U) 74,00 PDF
PR-ETA10050-03 TEV Protease(TurboTEV), recombinant, GST- & His-Tag 100 µg (1000 U) 109,00 PDF
PR-ETA10050-04 TEV Protease(TurboTEV), recombinant, GST- & His-Tag 1 mg (10.000 U) 424,00 PDF
  • All prices are in EURO excl. VAT and shipping. For further pricing and order information please ask your local distributor.

Literature

  1. Kapust et al. (2001) Tobacco etch virus protease: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency. Protein Eng. 14, 993-1000.