Non-covalent binding of biotinylated antibodies to M-Beads MIA-STA Beads
- Superparamagnetic beads with a mean diameter of 300 nm
- Not affected by reagent chemistry or photobleaching
- Excellent long-term stability
- Allows for magnetic immunoassays in a variety of formats
- Signal measured by a magnetometer is proportional to the analyte
- Can be used as
- detection label in magnetic immunoassays
- solid support phase in immunoassays with other readout techniques
- M-Beads MIA-STA beads are superparamagnetic beads with a mean diameter of 300 nm which can be used as a detection label in magnetic immunoassays, or as solid support phase in immunoassays with
other readout techniques.
- M-Beads MIA-STA 300 are coated with streptavidin and intended for use with biotinylated antibodies. Binding of molecules onto M-Beads MIA-STA 300 is based on the strong non-covalent interaction
between streptavidin and biotin (Ka = 1015 M-1). Biotin is easily conjugated to various molecules, and inexpensive biotinylated products are sold by many companies.
M-Beads MIA-STA beads are coated
with recombinant streptavidin (53 kDa) with truncated N- and C-terminus for improved solubility and accessibility of the sites. Albumin binding sites are eliminated for optimal specificity.
- Magnetic immunoassays (MIA) use magnetic beads as labels instead of conventional enzymes (ELISA), fluorophores, or luminescent molecules. These assays involve the specific binding of an antibody with
a conjugated magnetic label to its antigen. The presence of magnetic beads is then detected by a magnetic reader (magnetometer) which measures the magnetic field change induced by the beads. The signal
measured by the magnetometer is proportional to the analyte (virus, toxin, bacteria, cardiac marker, etc.) quantity in the initial sample.