Minute™ Mitochondria Membrane Protein Isolation Kit
MinuteTM Mitochondria Membrane Protein Isolation Kit (order# MP-007)
Enrichment of Mitochondrial Marker (Ubiquinol-Cytochrome C Reductase Core Protein) with Minute™ Mitochondria Isolation Kit.
A. SDS-PAGE profiles of total protein extract vs. isolated mitochondrial proteins. Lanes 1, 3, 5: total proteins from isolated mouse kidney cells, mouse skeletal muscle and liver tissues respectively. Lanes 2, 4, 6: isolated mitochondrial proteins from mouse kidney cells, mouse skeletal muscle and liver tissues respectively.
B. Western blotting. Proteins in A were transferred to nitrocellulose membranes and probed with anti-ubiquinol-cytochrome C reductase core protein (ab96333, Abcam, Cambridge, MA) and anti-Lamin B1 (ab16048, a nuclear envelope marker protein. Abcam, Cambridge, MA).
C. Densitometry measurement of mitochondrial marker signals.
- Invent Biotechnologies Minute™ mitochondria membrane protein isolation kit is composed of optimized buffers and protein extraction filter cartridges with 2.0 ml collection tubes. The kit is designed to rapidly isolate native mitochondria proteins from cultured mammalian cells or tissues. Due to the use of protein extraction filter cartridges, the protein isolation procedure is simple, easy and user friendly with high yield. Unlike many commercial mitochondria preparation kits, this kit offers wide range of starting cells (5-50 million/sample) and intact mitochondria are isolated. The buffers are detergent and EDTA free. A Dounce homogenizer or a tissue blender is not needed. The procedure can be completed in less than 30 min.
- The kit is designed to rapidly isolate mitochondria membrane proteins from cultured cells or tissues for applications such as SDS-PAGE, immunoblottings, ELISA, IP, membrane protein structure analysis, 2-D gels, enzyme activity assays and other applications.
|Buffer A||15 ml||MP-007-A|
|Buffer B||30 ml||MP-007-B|
|Protein extraction filter cartridges||50||P-001|
|Collection tubes with cap||50||P-002|
|Tissue dissociation beads||10 g||P-004|