Minute™ Nuclear Envelope Protein Extraction Kit
Minute™ Nuclear Envelope Protein Extraction Kit (order# NE-013)
A. SDS-PAGE profiles and Western blottings of isolated mouse spleen cells. Lanes 1 and 2: total cell lysate; Lane 3: nuclear envelope.
B. SDS-PAGE profiles and Western blottings of Rat (REL-6TN) and human (MCF-7) cell lines. Lane 1: REL-6TN total cell lysate; Lane 2: REL-6TN nuclear envelope. Lane 3: MCF-7 total cell lysate; Lane 4: MCF-7 nuclear envelope. Nuclear envelope marker Lamin B1 (ab 16048, Abcam, Cambridge MA) and GAPDH (G9545, Sigma) were visualized by a colorimetric substrate (Bio-Rad).
- The nuclear envelope is a very complex membrane-protein system that is notoriously difficult to isolate and purify due to its connection to nuclear and cytoplasmic components. Traditional method of nuclear envelope isolation and purification requires a large amount of starting material and a lengthy and tedious procedure. Invent Biotechnologies Minute™ nuclear envelope protein extraction kit is the first commercial kit designed to rapidly isolate nuclear envelope and its associated proteins in native form without using density-gradient and ultra centrifugation. Due to the use of protein extraction filter cartridges, the nuclear envelope protein isolation is simple, easy and user friendly. The nuclear envelope proteins are significantly enriched in the final prep. Unlike traditional method that requires large amount of starting cells/tissues this kit starts with only 10-20 million cells and the buffers are detergent and EDTA free. The procedure can be completed in less than 45 min with a final yield of 10-50 µg protein/sample.
- The kit is designed to rapidly isolate native nuclear envelope proteins from cultured cells or cells isolated from tissues for applications such as SDS-PAGE, immunoblottings, ELISA, IP, protein translocation analysis, enzyme activity assays and other applications. This kit provides the most rapid method currently available for enrichment of native nuclear envelope proteins.
|Buffer A||25 ml||NE-013-A|
|Buffer B||25 ml||NE-013-B|
|Protein extraction filter cartridges||50||P-001|
|Collection tubes with cap||50||P-002|