Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

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Protein and Nucleic Acid Extraction

Minute™ Protein and/or Nucleic Acid Extraction Kit from Gels

Minute™ Protein/Nucleic Acid Extraction Kit from Gels

Minute™ Protein/Nucleic Acid Extraction Kit from Gels (order# PN-019)

Pre-stained protein markers separated in 10% SDS-PAGE

Pre-stained protein markers (15 µg/lane) were separated in 10% SDS-PAGE.
Protein bands from two lanes were excised and extracted as described in the protocol. Proteins in the gels were eluted with 40 µl ddH2O. Eluted Proteins were separated in 10% SDS-PAGE without concentration. 10 µl pre-stained protein marker was loaded in this figure.

Description

  • Passive elution and electro elution are the most common methods for extracting proteins and/or nucleic acid from polyacrylamide/agarose gels. Protein extraction by passive elution usually takes overnight incubation. Eluted proteins are significantly diluted and require further concentration. Protein extraction by electro elution is faster (30 min to 2 h) than passive elution but it requires a special elution device that works less effectively for larger proteins (>70 kDa). The electro buffer usually contains detergent and other chemicals at a concentration that may interfere with downstream applications. Here we feature a rapid and instrument-free protein/nucleic acid extraction kit. Proteins/nucleic acid can be extracted from gels in <10 min with high yield. The elution buffer can be a detergent-containing buffer or pure water depending upon methods of gel staining. The elution volume is between 10 to 200 µl. Multiple gel pieces can be processed in a single tube and the final protein concentration is relatively high.

Application

  • Extracted proteins can be used for MALD-MS analysis, immunization of animals, protein-protein interaction and protein-nucleic acid interaction studies etc. Extracted nucleic acid can be used for PCR, cloning, and sequencing etc.

Kit Components:

Description Amount
Filter Cartridges 20
Microfuge Tube 20
Extraction Powder 2 g
Micro-pestles 5

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
PN-019 Minute™ Protein/Nucleic Acid Extraction Kit from Gels (20 Tests) 20 Tests 145,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in Europe. For further pricing and order information please ask your local distributor.