Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

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Protein Extraction

Minute™ Total Protein Extraction Kit for Animal Cultured Cells and Tissues

Minute™ Total Protein Extraction Kit for Animal Cultured Cells/Tissues

Minute™ Total Protein Extraction Kit for Animal Cultured Cells/Tissues (order# SD-001_SN-002)

Protein profiles of animal tissues

Protein profiles of animal tissues extracted by denaturing lysis buffer and native lysis buffer.
Extracted proteins were separated in 10% SDS-PAGE and stained with coomassie blue. Lane 1: drosophila larvae; Lane 2: drosophila pupa; Lane 3: adult drosophila; Lane 4: gold fish muscle; Lane 5: mouse liver

NIH 3T3 cell lysate prepared by sonication

Lane 1: NIH 3T3 cell lysate prepared by sonication; Lane 2 and 3: 3T3 cells prepared by Minute™ Denaturing Total Protein Extraction Kit (#SD-001)

293T cell proteins prepared by Minute™ Denaturing Total Protein Extraction Kit

Lane 1: 293T cell proteins prepared by Minute™ Denaturing Total Protein Extraction Kit (#SD-001). Lane 2 and 3: 293T cell protein prepared by Minute™ Native Total Protein Extraction Kit (#SN-002)

Description

  • Invent Biotechnologies' Minute™ total protein extraction kit for animal cultured cells and tissues is composed of optimized cell lysis buffer and protein extraction filter cartridges with 2.0 ml collection tubes. The kit is designed to rapidly extract total proteins from animal cells and tissues (invertebrate and vertebrate) for protein analysis and further purification. Since protein profiles extracted by denaturing and native cell lysis buffer are not identical, for a given application one might be superior to the other. This kit provides both denaturing and native cell lysis buffers for users to test and select the best one for a specific application. Due to the use of the protein extraction filter cartridges, the extraction volume can be as small as 20 µl and as large as 500 µl. This unique feature is very useful in situations where available starting material is a limiting factor. Total proteins can be extracted from cultured cells/tissues in 1-8 min with high yield (2-8 mg/ml). Extracted proteins can be use as a good starting material for protein analysis and small scale protein purification in column chromatography.

Application

  • Minute™ total protein extraction kit is designed to rapidly extract total proteins from invertebrate and vertebrate cultured cells and tissues for applications such as SDS-PAGE, immunoblottings, IP, ELISA, enzyme assays and other applications. This kit provides the most rapid method currently available for preparation of whole cell protein extract. Extracted proteins can also be use as a good starting material for small scale protein purification in column chromatography.

Kit Components:

Description Amount Part #
Denaturing cell lysis buffer 25 ml SD-001-L
Native cell lysis buffer 25 ml SN-002-L
Protein extraction filter cartridges 50 P-001
Collection tubes with cap 50 P-002
Plastic rods 4 P-003

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
SD-001_SN-002 Minute TM Total Protein Extraction Kit for Animal Cultured Cells/Tissues (50 Tests) 50 Tests 240,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in Europe.