Innovative Tools for Molecular and Cell Biology

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Protein Extraction

Minute™ Total Protein Extraction Kit for Microbes with Thick Cell Walls

Minute Total Protein Extraction Kit for Microbes with Thick Cell Walls

MinuteTM Total Protein Extraction Kit for Microbes with Thick Cell Walls (order# YT-015)

SDS-PAGE profiles of extracted proteins

SDS-PAGE (12%) profiles of extracted proteins.
A (denaturing). Lane 1: S. cerevisiae; Lane 2: filamentus fungus; Lane 3: L. lactis (Gram+)
B (native). Lane 1: S. cerevisiae; Lane 2: L. lactis

Description

  • Protein extraction from cell wall-containing microbes is a frequent procedure in bio-research Labs. The methods for protein extraction from these microbes are usually harsh, tedious and not sufficiently reliable. Minute™ Total Protein Extraction Kit for Microbes with Thick Cell Walls (#YT-015) provides an instrument-free, rapid and gentle way for extracting proteins from microbes with thick and strong cell walls. These microbes include but not limited to yeast, filamentous fungus, gram positive and negative bacteria, insect eggs and microalgae. This kit contains optimized denaturing and native protein extraction buffers for user to choose from. Unlike many other methods of which harsh conditions, such as 8M urea, glass bead lysis using an instrument, and boiling the sample with alkaline extraction etc., are used for yeast protein extraction. Proteins in certain molecular weight range are usually preferentially extracted with these methods. This kit features a single tube protocol, full range of yeast proteins are extracted without bias. The kit works well for both log phase and stationary phase microbes. The whole procedure takes less than 10 min to complete and the protein yield is in the range of 2-5 mg/ml. The materials provided are sufficient for 50 extractions.

Application

  • Proteins extracted with this kit can be directly used for many downstream applications such as SDS-PAGE analysis, Western blotting, IP. ELISA and enzyme activity assays and proteomic analysis. The buffers are compatible with IMAC resins for his-tagged protein purification.

Kit Components:

Description Amount
Denaturing buffer 20 ml
Native buffer 20 ml
Protein extraction powder 5 g
1.5 ml microfuge tube 50
Pestles 4

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
YT-015 Minute™ Total Protein Extraction Kit for Microbes with Thick Cell Walls (50 Tests) 50 Tests 145,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in Europe. For further pricing and order information please ask your local distributor.