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Protein Extraction

Minute™ Total Protein Extraction Kit for Plant Tissues

Total Protein Extraction Kit for Plant Tissues

MinuteTM Total Protein Extraction Kit for Plant Tissues (order# SD-008_SN-009)

Plant protein profiles

Plant protein profiles extracted by denaturing lysis buffer and native lysis buffer.
Extracted proteins were separated in 10% SDS-PAGE and stained with coomassie blue. Lane 1: snow pea; Lane 2: corn kernel; Lane 3: broccoli leaves;Lane 4: spinach leaves and Lane 5: chili pepper leaves.

Description

  • Invent Biotechnologies Minute™ total protein extraction kit for plant tissues is composed of optimized protein extraction buffer and protein extraction filter cartridges with 2.0 ml collection tubes. The kit is designed to rapidly extract denatured or native proteins from plant tissues (leaves, seeds and soft stem and roots etc.). Since protein profiles extracted by denaturing and native cell lysis buffer are not identical, for a given application one might be superior to the other. This kit provides both denaturing and native cell lysis buffers for users to test and select the best one for a specific application. Due to the use of the protein extraction filter cartridges, total plant soluble proteins can be extracted from 20-200 mg plant tissue in less than 8 min with high protein yield (2-8 mg/ml). Extracted proteins can be use as a good starting material for protein analysis and small scale protein purification in column chromatography.

Application

  • Minute™ total protein extraction kit for plant tissues is designed to rapidly extract total proteins from fresh or frozen plant tissues for applications such as SDS-PAGE, immunoblottings, ELISA, IP, enzyme assays and other applications. This kit provides a very rapid method for preparation of soluble protein extract from plant tissues. Extracted proteins can be use as a good starting material for small scale protein purification in column chromatography.

Kit Components:

Description Amount Part #
Denaturing lysis buffer 25 ml SD-008-L
Native lysis buffer 25 ml SN-009-L
Protein extraction filter cartridges 50 P-001
Collection tubes with cap 50 P-002
Plastic rods 4 P-003

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
SD-008_SN-009 Minute TM Total Protein Extraction Kit for Plant Tissues (50 Tests) 50 Tests 240,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in Europe.