Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

Products

Protein Extraction

Minute™ Yeast Mitochondria Enrichment Kit

Minute Yeast Mitochondria Enrichment Kit

MinuteTM Yeast Mitochondria Enrichment Kit (order# YM-017)

SDS-PAGE profiles of S. cerevisiae fractions

SDS-PAGE (12%) profiles of S. cerevisiae fractions.
Lane 1: total cell lysate
Lane 2: cytosolic fraction
Lane 3: mitochondrial fraction

Description

  • Traditional protocols for yeast mitochondria isolation/extraction involve a range of centrifugation-based subcellular fractionation procedures. Typically the techniques include spheroplast preparation, glass-bead lysis using a homogenization instrument, differential centrifugation and several density gradient procedures using a variety of gradient media with ultracentrifugation. The procedures are very tedious and time consuming. Here we feature a simple and rapid protocol for yeast mitochondria enrichment. The procedure is gentle and instrument-free. Native mitochondrial proteins can be isolated from yeast in about one hour without ultracentrifugation. This kit contains optimized detergent-free protein extraction buffers. The protein yield is in the range of 150-250 µg/sample. The materials provided are sufficient for 50 extractions.

Application

  • Proteins extracted with this kit can be used for many downstream applications such as SDS-PAGE analysis, Western blotting, IP. ELISA, enzyme activity assays, proteomics and other biochemical analysis.

Kit Components:

Description Amount
Buffer A 30 ml
Buffer B 10 ml
Protein extraction powder 5 g
1.5 ml microfuge tube 50
Pestles 4

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
YM-017 Minute™ Yeast Mitochondria Enrichment Kit (50 tests) 50 Tests 288,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in Europe. For further pricing and order information please ask your local distributor.