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Innovative Tools for Molecular and Cell Biology

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Bacillus megaterium Constitutive Expression System

pPconst1326 Vector

Vector map of pPconst1326

Vector map of pPconst1326

Features

  • E. coli/ Bacillus shuttle vector
  • Target gene cloning in E. coli and protein production in Bacillus sp.
  • For constitutive expression of recombinant proteins in Bacillus megaterium

Description

  • pPconst1326 is an E. coli/ Bacillus shuttle vector for the constitutive expression of recombinant proteins in Bacillus megaterium. pPconst1326 can be used for cloning of a target gene in E. coli and protein production in Bacillus sp..
  • To generate the pPconst1326 constitutive vector the promoter of pyruvate dehydrogenase operon (pdhABCD, bmd_1326-1329) was isolated from B. megaterium DSM319 and cloned into an E. coli/ Bacillus shuttle vector. The promoter region is followed by the native ribosomal binding site (RBS) of the corresponding gene cluster including its start codon that is located upstream of multiple cloning site (MCS). First restriction site (SpeI) allows cloning of a gene of interest fused to nine additional nucleotides encoding Met-Thr-Ser at its N-terminus. The pPconst1326 carries the oriU and repU gene from Bacillus cereus for replication in Bacillus sp., the tetL gene providing resistance against tetracycline, and functional genetic elements for E. coli (origin of replication ColE1 ori and the bla gene encoding a ß-lactamase which provides resistance against ampicillin). Unlike inducible gene expression system the expression of the target protein is driven constitutively by the pdhABCD promoter. Thus, addition of an inducer is not required during the cultivation of B. megaterium cells.

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
BMEG45 Bacillus megaterium vector pPconst1326 10 µg 619,00 PDF
  • All prices are in EURO excl. VAT and shipping. For further pricing and order information please ask your local distributor.

Downloads

  • Bacillus megaterium Protein Production System Handbook (PDF)

Vector Maps and Sequences

Vector map of pPconst1326 (pic)

Sequence of pPconst1326 (txt)