SURE® Expression System for Bacillus subtilis
The SURE® System: Subtilin Regulated gene Expression
Fig. 1 Components of the SURE system
Fig. 2 Induction of gene expression in SURE after addition of subtilin to the culture (P = Phosphorylation)
- Tightly controlled gene expression in a bacterium with long history of biotechnology
- Genome sequence available
- Longstanding genetic engineering experience
- Complete set of genetic engineering tools available
- Subtilin producing strain cuts costs
- Potentially useful for cloning of genes with toxic products
- Potential for secretion of gene products
- Controlled gene expression for metabolic engineering
- All vectors are B. subtilis/E. coli shuttle vectors
Controlled gene expression in B. subtilis – a cost-saving alternative to IPTG induced gene expression
- IPTG (Isopropyl-ß-D-thiogalactopyranoside) is a widespread, established, and non-degradable inducer. However, since IPTG is a considerably expensive compound, an inexpensive method for induction is desirable.
- Subtilin is a small peptide antibiotic of 32 amino acids produced by B. subtilis. Subtilin production and regulation is encoded in the chromosome by a cluster of 9 genes, which are transcribed from two
promoters. Subtilin regulates/activates its own biosynthesis via a two component regulatory system.
For the SURE system the regulatory components called SpaR (response regulator) and SpaK (membrane sensor) were isolated and placed on the chromosome of a B. subtilis host strain. One of the subtilin regulated promoters is located upstream of a multiple cloning site into which the gene of interest can be cloned (gene X). Figure 1 shows the basic components of the SURE system.
- Upon addition of subtilin the system is activated and the protein of interest is produced. It accumulates either intracellularly (blue), or is secreted (green) into the medium (see Figure 2).
- For shipping and storage information please click on Order#.
|VS-ELS10610-01||NICE E. coli host Strain MC1061||1 ml||305,00|
|VS-ELS10710-01||MC1061 Chemically Competent Escherichia coli||5 x 100 µl||438,00|
|PBS023||Bacillus subtilis strain NZ8963||1 ml||529,00|
|PBS024||Bacillus subtilis strain NZ8900||1 ml||529,00|
|PBS025||Bacillus subtilis strain NZ8901||1 ml||350,00|
|PBS031||pNZ8901 vector, lyophilized DNA||10 µg||529,00|
|PBS032||pNZ8902 vector, lyophilized DNA||10 µg||529,00|
|PBS033||pNZ8910 vector, lyophilized DNA||10 µg||529,00|
|PBS034||pNZ8911 vector, lyophilized DNA||10 µg||529,00|
- All prices are in EURO excl. VAT and shipping. For further pricing and order information please ask your local distributor.
The SURE Gene Expression System for Bacillus subtilis - Handbook (PDF)
Vector maps and Sequences
- Bongers, R. S., J. W. Veening, M. Van Wieringen, O. P. Kuipers, and M. Kleerebezem. 2005. Development and characterization of a subtilin-regulated expression system in Bacillus subtilis: strict control of gene expression by addition of subtilin. Applied and Environmental Microbiology 71:8818-8824. Pubmed
- Kleerebezem, M. 2004. Quorum sensing control of lantibiotic production; nisin and subtilin autoregulate their own biosynthesis. Peptides 25:1405-1414. Pubmed
- Kleerebezem, M., R. Bongers, G. Rutten, W. M. de Vos, and O. P. Kuipers. 2004. Autoregulation of subtilin biosynthesis in Bacillus subtilis: the role of the spa-box in subtilin-responsive promoters. Peptides 25:1415-1424. Pubmed
- Mierau, I., and M. Kleerebezem. 2005. 10 years of the nisin-controlled gene expression system (NICE) in Lactococcus lactis. Applied Microbiology and Biotechnology 9:1-13. Pubmed
- Vavrova, L., Muchova, K., and Barák, I. 2010. Comparison of different Bacillus subtilis expression systems.
Res Microbiol. 161(9):791-7. Pubmed
- The patented SURE® system was developed by NIZO food research BV. SURE® is a trademark of NIZO food research BV.
- All SURE® products, developed by NIZO food research BV, are for academic research and development only.
- Commercial enterprises: To obtain strains and plasmids, a license needs to be negotiated.
For license agreements please contact firstname.lastname@example.org.