Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology


Broad-Host-Range Vectors

Broad-Host-Range Vectors pBBR122 and pBHR1

Vector map of pBBR122

Vector map of pBBR122

Vector map of pBHR1

Vector map of pBHR1


  • Expression in bacteria other than E. coli (mainly Gram-negative)
  • Change of bacterial host
  • Studies of broad-host-range replicons
  • Studies of Gram-negative bacteria


  • pBBR122 is a cloning vector with very broad host-range maintenance. As opposed to other known broad-host-range vectors, it replicates at medium copy numbers and has a small size (5305 bp). This greatly facilitates genetic studies of a wide variety of Gram-negative bacteria and makes pBBR122 particularly interesting for studies of broad-host-range replicons. It stably replicates in all Gram-negative organisms tried so far under standard growth conditions using the appropriate selective pressure.
  • pBBR122 was derived from pBBR1, which was isolated from Bordetella bronchiseptica S87. It is compatible with other broad-host-range vectors, since it does not belong to any of the broad-host-range incompatibility groups IncP, IncQ or IncW.

Genetic information

  • pBBR122 is neither mobilizable nor conjugative. Rep is the gene involved in replication. By removing a frame shift in the mob gene of pBBR122, pBHR1 was created. This mobilizable vector is available as well.

pBBR122 and pBHR1 replicate in

  • Aeromonas caviae
  • Aeromonas hydrophila
  • Acetobacter xylinum
  • Aeromonas veronii biovar sobria
  • Agrobacterium tumefaciens
  • Alcaligenes eutrophus
  • Azorhizobium caulinodans
  • Bartonella bacilliformis
  • Bordetella spp
  • Brucella spp
  • Caulobacter crescentus
  • Escherichia coli
  • Hyphomicrobium denitrificans
  • Hyphomicrobium facilis
  • Methylobacillus glycogenes
  • Methylbacterium extorquens
  • Methylophilus methylotrophus
  • Paracoccus denitrificans
  • Pseudomonas denitrificans
  • Pseudomonas fluorescens
  • Pseudomonas putida
  • Pseudomonas (Burkholderia) solanacearum
  • Pseudomonas syringae
  • Rhizobium leguminosarum
  • Rhizobium meliloti
  • Rhodobacter sphaeroides
  • Salmonella typhimurium
  • Vibrio cholerae
  • Xanthomonas campestris
  • and potentially many more!


  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
PBBR01 pBBR122 Broad Host Range Vector, lyophilized DNA 5 µg 367,00 PDF
PBBR02 pBHR1 vector DNA (mobilizable), lyophilized 5 µg 416,00 PDF
  • All prices are in EURO excl. VAT and shipping. For further pricing and order information please ask your local distributor.



Sequence of pBHR1 (txt)
Sequence of pBBR122 (txt)