Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

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Poly(His)-Tag Cloning Vector pEG-His1

Fast and Easy Purification of Full Length Recombinant Proteins from E. coli

pEG-His1 vector

Vector map of pEG-His1

Features

  • Optimized promoter guarantees excellent expression levels
  • Very tight expression control due to overexpression of the Lac I repressor
  • A convenient MCS allows flexible and easy cloning of the insert
  • Start codon is provided by an NdeI site
  • Inserts can be expressed as C-terminal tagged 6xHis fusion proteins for efficient and easy one-step purification of full length proteins by metal-chelate affinity chromatography
  • An RGS motive and the proximate Poly(His)-Tag allow detection and/or immunoprecipitation of the expressed protein with commercial anti-RGS and anti-His antibodies

Description

  • The pEG-His1 vector was constructed for the expression of toxic gene products in E. coli. To obtain its exceptional tightness prior to induction with IPTG, the lac I repressor gene has been included in the vector and is overexpressed in plasmid-bearing cells.
  • Recombinant fusion proteins with a 6xHis tag can be easily and selectively purified using the unique Ni-NTA (nickel-nitrilotriacetic acid) technology. The Ni-NTA affinity matrix shows an optimal binding capacity and a minimized non-specific binding resulting in highly purified and reproducible 6xHis-tagged protein preparations.

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
PEG01 pEG-His 1 vector 5 µg 242,00 PDF
  • All prices are in EURO excl. VAT and shipping. For further pricing and order information please ask your local distributor.

  • The vector comes with 500 pmol of 5' and 3' sequencing primers each.

DOWNLOADS

  • Sequence of pEG-His1 (txt)
  • Vector map of pEG-His1 (PDF)